Influence of hydroxyapatite granule size, porosity, and crystallinity on tissue reaction <i>in vivo</i>. Part A: synthesis, characterization of the materials, and <scp>SEM</scp> analysis

Val, José Eduardo Maté Sánchez de; Calvo-Guirado, José Luís; Gómez‐Moreno, Gerardo; Martínez, Carlos Pérez-Albacete; Mazón, Patricia; Aza, Piedad N. De

doi:10.1111/clr.12722

Cited by 49 publications

(53 citation statements)

References 39 publications

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“…The figure includes Type I collagen for comparison purposes. Above 1300 cm −1 , almost all the bands are exclusively assigned to collagen vibrations, the exception being those brought about by CO 3 2− at 1460 cm −1 , and a broad band present at 3500, which is attributed to the presence of the structural OH- groups [12,45,46]. …”

Section: Resultsmentioning

confidence: 99%

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SEM-EDX Study of the Degradation Process of Two Xenograft Materials Used in Sinus Lift Procedures

Fernández

Gehrke²,

Martı́nez

et al. 2017

Materials

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Some studies have demonstrated that in vivo degradation processes are influenced by the material’s physico-chemical properties. The present study compares two hydroxyapatites manufactured on an industrial scale, deproteinized at low and high temperatures, and how physico-chemical properties can influence the mineral degradation process of material performance in bone biopsies retrieved six months after maxillary sinus augmentation. Residual biomaterial particles were examined by field scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) to determine the composition and degree of degradation of the bone graft substitute material. According to the EDX analysis, the Ca/P ratio significantly lowered in the residual biomaterial (1.08 ± 0.32) compared to the initial composition (2.22 ± 0.08) for the low-temperature sintered group, which also presented high porosity, low crystallinity, low density, a large surface area, poor stability, and a high resorption rate compared to the high-temperature sintered material. This demonstrates that variations in the physico-chemical properties of bone substitute material clearly influence the degradation process. Further studies are needed to determine whether the resorption of deproteinized bone particles proceeds slowly enough to allow sufficient time for bone maturation to occur.

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Section: Resultsmentioning

confidence: 99%

“…However, no clear insight has yet been provided as to the exact relationship between the material properties of these calcium phosphate ceramics and their degradation behavior [12]. Therefore, long-term studies must be carried out to understand the pattern of biodegradation of xenografts and their influence on bone gain.…”

Section: Introductionmentioning

confidence: 99%

SEM-EDX Study of the Degradation Process of Two Xenograft Materials Used in Sinus Lift Procedures

Fernández

Gehrke²,

Martı́nez

et al. 2017

Materials

View full text Add to dashboard Cite

show abstract

“…The figure includes Type I collagen for comparison purposes. Above 1300 cm -1 , almost all the bands are exclusively assigned to collagen vibrations, the exception being those brought about by CO3 2-at 1460 cm -1 , and a broad band present at 3500, which attributes to the presence of the structural OH-groups [12,45,46]. Figure 3 shows the xenograft materials before insertions for maxillary sinus floor elevation.…”

Section: Characterization Of Deproteinized Hydroxyapatite Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

SEM-EDX Study of the Degradation Process of Two Xenograft Materials Used in Sinus Lift Procedures

Fernández¹,

Gehrke²,

Martı́nez³

et al. 2017

Preprint

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Some studies have demonstrated that in vivo degradation processes are influenced by the material's physico-chemical properties. The present study compares two hydroxyapatites manufactured on an industrial scale, deproteinized at low and high temperatures, and how physicochemical properties can influence the mineral degradation process of material performance in bone biopsies retrieved 6 months after maxillary sinus augmentation. Residual biomaterial particles were examined by field scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) to determine the composition and degree of degradation of the bone graft substitute material. According to the EDX analysis, the Ca/P ratio significantly lowered in the residual biomaterial (1.08±0.32) compared to the initial composition (2.22±0.08) for the low-temperature sintered group, which also presented high porosity, low crystallinity, low density, a large surface area, and poor stability and a high resorption rate compared to the high-temperature sintered material. This demonstrates that variations in the physico-chemical properties of bone substitute material clearly influence the degradation process. Further studies are needed to determine whether the resorption of deproteinized bone particles proceeds slowly enough to allow sufficient time for bone maturation to occur.

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“…Typically, Si-doped HA and Si-TCP ceramics have been prepared, although both pure Ca-P ceramics and Si-doped Ca-P ceramics still have shortcomings. Previous studies have shown that sintered HA does not significantly degrade, but remains a permanent fixture, susceptible to long-term failure [11,12,13]. Although α and β-TCP ceramics are degradable at a quicker degradation rate than HA, in vivo osteogenesis of sintered α and β-TCP ceramics is far from optimal.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells

et al. 2016

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A new type of bioceramic with osteogenic properties, suitable for hard tissue regeneration, was synthesised. The ceramic was designed and obtained in the Nurse’s A-phase-silicocarnotite subsystem. The selected composition was that corresponding to the eutectoid 28.39 wt % Nurse’s A-phase-71.61 wt % silicocarnotite invariant point. We report the effect of Nurse’s A-phase-silicocarnotite ceramic on the capacity of multipotent adult human mesenchymal stem cells (ahMSCs) cultured under experimental conditions, known to adhere, proliferate and differentiate into osteoblast lineage cells. The results at long-term culture (28 days) on the material confirmed that the undifferentiated ahMSCs cultured and in contact with the material surface adhered, spread, proliferated, and produced a mineralised extracellular matrix on the studied ceramic, and finally acquired an osteoblastic phenotype. These findings indicate that it underwent an osteoblast differentiation process. All these findings were more significant than when cells were grown on plastic, in the presence and absence of this osteogenic supplement, and were more evident when this supplement was present in the growth medium (GM). The ceramic evaluated herein was bioactive, cytocompatible and capable of promoting the proliferation and differentiation of undifferentiated ahMSCs into osteoblasts, which may be important for bone integration into the clinical setting.

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Influence of hydroxyapatite granule size, porosity, and crystallinity on tissue reaction in vivo. Part A: synthesis, characterization of the materials, and SEM analysis

Abstract: The data from this study show that changing the size, porosity, and crystallinity of one HA-based bone substitute material can influence the integration of the biomaterials within the implantation site and the new bone formation.

Cited by 49 publications

References 39 publications

SEM-EDX Study of the Degradation Process of Two Xenograft Materials Used in Sinus Lift Procedures

SEM-EDX Study of the Degradation Process of Two Xenograft Materials Used in Sinus Lift Procedures

SEM-EDX Study of the Degradation Process of Two Xenograft Materials Used in Sinus Lift Procedures

Assessment of Effects of Si-Ca-P Biphasic Ceramic on the Osteogenic Differentiation of a Population of Multipotent Adult Human Stem Cells

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