Influence of hydrocolloidal silver nanoparticles on gastrointestinal microflora and morphology of enterocytes of quails

Sawosz, Ewa; Binek, M.; Grodzik, Marta; Zielińska, Monika; Sysa, P.; Szmidt, Maciej; Niemiec, Tomasz; Chwalibog, A.

doi:10.1080/17450390701664314

Cited by 102 publications

(78 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This lack of treatment effect is consistent with findings in quail, pigs, and chickens and supports the conclusion that AgNano do not affect growth [7,20,[23][24][25]. Interestingly, the difference in fat uptake between treatments was not reflected in hatchling development.…”

Section: Hatchling Developmentsupporting

confidence: 80%

Investigating the Effect of In Ovo Injection of Silver Nanoparticles on Fat Uptake and Development in Broiler and Layer Hatchlings

Pineda

Chwalibog

Sawosz

et al. 2012

Journal of Nanotechnology

Self Cite

View full text Add to dashboard Cite

Silver nanoparticles (AgNano) as carrier of available oxygen (O 2 ) and with high surface reactivity may increase O 2 consumption, enhance fat uptake (FU), and stimulate growth and development. The objective was to investigate the effects of in ovo injection of AgNano on the metabolic rate (O 2 consumption, CO 2 production, and heat production, HP), fat uptake, and the development of broiler and layer hatchlings. AgNano concentrations (50, 75, and 100 mg/kg) were injected in ovo at day 1 of incubation to different breeds of broiler and layer chicken embryos. Oxygen consumption and subsequently FU did not increase linearly following AgNano treatment. FU was lower in hatchlings treated with 50 and 100 mg AgNano/kg, but surprisingly not in hatchlings treated with 75 mg AgNano/kg. Interestingly, the difference in FU between treatments was not reflected in hatchling development. The results indicated that AgNano affected metabolic rate and FU; however, it did not influence the development of hatchlings. This suggests that in ovo injection of AgNano reduces the need to use yolk fat as an energy source during embryonic development and consequently the remaining fat in the residual yolk sac may provide a potent source of nutritional reserves for chicks of few days after hatching.

show abstract

Section: Hatchling Developmentsupporting

confidence: 80%

Investigating the Effect of In Ovo Injection of Silver Nanoparticles on Fat Uptake and Development in Broiler and Layer Hatchlings

Pineda

Chwalibog

Sawosz

et al. 2012

Journal of Nanotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…histolyticum group was reduced with 20 ppm silver (P = 0.012). In the same way, Sawosz et al (2007) did not observed a major effect of colloidal silver on bacterial concentration in the digestive tract of quails, but only a significant increase in lactic acid bacteria was observed with 25 ppm. Results on productive performances in several experiments with pigs and poultry carried out by our group were variable (Table 1): a numerical increase in daily growth was generally observed when 20 ppm silver were added compared with the control (no silver), but this effect was not generally significant.…”

Section: Potential Use Of Silver In Animal Feedingsupporting

confidence: 49%

Potential Use of Silver Nanoparticles as an Additive in Animal Feeding

Fondevila¹

2010

Silver Nanoparticles

View full text Add to dashboard Cite

Among other uses, metallic silver and silver salts have currently been applied as antimicrobial agents in many aspects of medical industries, such as coating of catheters, dental resin composites and burn wounds, as well as in homeopathic medicine, with a minimal risk of toxicity in humans. However, their use in animal feeding as prebiotics have remain minimised, mostly because of the low cost antibiotics used as growth promoters in the second half of the XX Century. However, after the ban of this practice in the European Community, silver compounds appear as a potential alternative to other already in use, such as organic acids, oligosaccharides, plant extracts, etc. The major concerns about the safe use of an additive in animal feeding are its effective role as antimicrobial, acting selectively over potential pathogens but not over symbiotic microbial communities; a low toxic effect over the animal and its human consumer; and a low risk of environmental pollution. Metallic silver nanoparticles (up to 100 nm) allow for a higher antimicrobial effect than silver salts, are more resistant to deactivation by gastric acids and have a low absorption rate through the intestinal mucosa, thus minimising its potential risk of toxicity. Besides, it has been shown that the doses that promote animal physiological and productive effects are very low (20 to 40 ppm), especially compared to the 10 to 100-fold higher concentration used with other metallic compounds such as copper and zinc, thus precluding a harmful environmental effect. This chapter describes the reasons why silver nanoparticles could be applied to animal feeding, and provides with some available data in this regard. In any case, its registration as feed additive is a previous requisite before being applied in practical conditions.

show abstract

“…33 More and more results provide evidence for a molecular mechanism of nano-Ag activity, showing that nano-Ag act through reactive oxygen species generation, 29 leading to activation of proteins and inducing apoptosis via the mitochondrial pathway. 34 In vivo measurements in chicken embryos and quails demonstrated that hydrocolloids of nano-Ag at the level of 50 ppm did not affect development or cause oxidative DNA damage to embryos 35 or growth of quails. 36 Gold nanoparticles (nano-Au) are well established carriers for delivery of double-stranded DNA in gene gun technology.…”

Section: Introductionmentioning

confidence: 99%

Visualization of interaction between inorganic nanoparticles and bacteria or fungi

Chwalibog¹,

Sawosz²,

Hotowy³

et al. 2010

IJN

257

149

View full text Add to dashboard Cite

Purpose:The objective of the present investigation was to evaluate the morphologic characteristics of self-assemblies of diamond (nano-D), silver (nano-Ag), gold (nano-Au), and platinum (nano-Pt) nanoparticles with Staphylococcus aureus (bacteria) and Candida albicans (fungi), to determine the possibility of constructing microorganism-nanoparticle vehicles. Methods: Hydrocolloids of individual nanoparticles were added to suspensions of S. aureus and C. albicans. Immediately after mixing, the samples were inspected by transmission electron microscopy. Results: Visualization of the morphologic interaction between the nanoparticles and microorganisms showed that nano-D, which are dielectrics and exhibit a positive zeta potential, were very different from the membrane potentials of microorganisms, and uniformly surrounded the microorganisms, without causing visible damage and destruction of cells. All metal nanoparticles with negative zeta potential had cell damaging properties. Nano-Ag showed the properties of self-organization with the cells, disintegrating the cell walls and cytoplasmic membranes, and releasing a substance (probably cytoplasm) outside the cell. Arrangement of nano-Au with microorganisms did not create a system of self-organization, but instead a "noncontact" interaction between the nanoparticles and microorganisms was observed to cause damage to fungal cells. Nano-Pt caused both microorganisms to release a substance outside the cell and disintegrated the cytoplasmic membrane and cell wall. Conclusion: Nano-Ag, nano-Au, and nano-Pt (all metal nanoparticles) are harmful to bacteria and fungi. In contrast, nano-D bind closely to the surface of microorganisms without causing visible damage to cells, and demonstrating good self-assembling ability. The results indicate that both microorganisms could be used as potential carriers for nano-D.

show abstract

Influence of hydrocolloidal silver nanoparticles on gastrointestinal microflora and morphology of enterocytes of quails

Cited by 102 publications

References 14 publications

Investigating the Effect of In Ovo Injection of Silver Nanoparticles on Fat Uptake and Development in Broiler and Layer Hatchlings

Investigating the Effect of In Ovo Injection of Silver Nanoparticles on Fat Uptake and Development in Broiler and Layer Hatchlings

Potential Use of Silver Nanoparticles as an Additive in Animal Feeding

Visualization of interaction between inorganic nanoparticles and bacteria or fungi

Contact Info

Product

Resources

About