“…This efficiency can be altered as a result of time out after anthesis (García et al, 2000); growth regulators Nookaraju et al, 2007); grape variety used (Hewstone et al, 2006); intensity and type of pruning (Dalal et al, 1992;Ponce et al, 2009); genotype, harvest time and embryo size (Ramming and Emershad, 1990;Pommer et al, 1995) thus culture medium and time of in vitro culture (Valdez, 2005). Based on the above, the present study aims to evaluate the efficiency of embryo rescue technique in f1 individuals from hybridizations performed through classical genetic techniques using different varieties of seedless grapes.…”