Influence of genotype and processing on bioactive compounds of Ethiopian specialty Arabica coffee

Wondimkun, Yishak Worku; Emire, Shimelis Admassu; Teferra, Tadesse Fikre; Stoecker, Barbara J.; Esho, Tarekegn Berhanu

doi:10.1080/10942912.2022.2147540

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…Concerning marketing and quality traits, sensory attributes of coffee beans are influenced by the chemical composition of green beans, which in turn is controlled by genetic factors (Ky et al, 2001;Leroy et al, 2006;Farah, 2009;Tran et al, 2017), environment production, fruit maturation physiology and technological factors such as post-harvest processing (Kitzberger et al, 2020;Wondimkun et al, 2022) or even by different methods of coffee brewing (Novaes et al, 2019;Chavez et al, 2022). It is known that within the species C. arabica (Ky et al, 2001;Scholz et al, 2016) and C. canephora (Mori et al, 2016) there is genetic variability for nonvolatile biochemical precursors of coffee aroma such as caffeine and trigonelline, chlorogenic acids, sucrose, and lipids such as cafestol and kahweol diterpenes (Ky et al, 2001;Farah, 2009).…”

Section: Gene Editing Of Coffeementioning

confidence: 99%

CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane

Prado,

Rocha,

Santos

et al. 2024

Front. Plant Sci.

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Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars.

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