Influence of environmental exposure to PAHs on the susceptibility of lymphocytes to DNA-damage induction and on their repair capacity

Cebulska-Wasilewska, Antonina; Wiecheć, A.; Panek, Agnieszka; Binková, Blanka; Šrám, Radìm J.; Farmer, Peter B.

doi:10.1016/j.mrgentox.2005.08.013

Cited by 38 publications

(11 citation statements)

References 22 publications

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“…Binkova et al 42 found in a group of healthy non-smoking women, living in an area polluted by PAHs, the average level of 5.35 and 7.12 %tDNA (DNA-SSBs; medians). Other authors studied a group of policemen who were exposed to combination of PAHs and ultraviolet radiation 43 . In summer they found 2.91±1.05 %tDNA (DNA-SSBs; mean±SD), in winter 2.42±1.70 %tDNA.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation

Málková¹,

Köhlerová²,

Fiala³

et al. 2016

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Aims. Goeckerman therapy is based on combined exposure to UV radiation (UVA, UVB) and crude coal tar (PAHs). Some indicators suggest a genotoxic hazard, however, the level of genotoxic risk of the therapy has not yet been investigated sufficiently. This study aims to assesss the genotoxic risk. Methods. The studied group consisted of patients with chronic stable plaque psoriasis treated by Goeckerman therapy (n = 29). Heparin-treated peripheral blood samples were collected one day before the first treatment and immediately after the last procedure. The lymphocytes were isolated from the blood. The level of genotoxicity was evaluated using an alkaline version of the Comet assay which detects DNA single strand breaks (DNA-SSBs), a neutral version of the Comet assay which detects DNA double strand breaks (DNA-DSBs), and using chromosomal aberrations. Results. The level of DNA-SSBs increased insignificantly (median; Q1-Q3): 1.4 (0.4; 0.1-1.4) vs. 2.5 (0.6; 0.3-2.7) %tDNA (P = 0.11) and the level of DNA-DSBs increased significantly: 7.8 (6.5; 3. 4-10.5) vs. 20.7 (19.3; 14.2-24.6) % DNA (P < 0.001). The total number of aberrated cells (P < 0.001) and structurally aberrated cells (P < 0.001) increased significantly. Conclusion. The elevated levels of the DNA-DSBs and the chromosomal aberrations in the peripheral lymphocytes indicated a genotoxic hazard. However, the elevated level of the chromosomal abnormalities was below the upper level of the reference range for healthy Czech adults. While, the genotoxic risk appears to be low, Goeckerman treatment represents a further contribution to the lifetime load of genotoxic factors.

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Section: Discussionmentioning

confidence: 99%

Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation

Málková¹,

Köhlerová²,

Fiala³

et al. 2016

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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“…The most common approach is to compare similar population groups living in polluted and clean areas. In some cases, individuals were compared on the basis of their occupation as being more or less exposed to ambient pollution [17][18][19]. A few studies included measurement of exposure, and looked for correlations with levels of DNA damage [17][18].…”

Section: Discussionmentioning

confidence: 99%

“…Arayasiri et al [17] compared 24 Bangkok traffic police with 24 office-based police, and found 70% higher levels of SBs in the outdoor workers. Police in Prague were selected for their occupational exposure to polycyclic hydrocarbons, and compared with subjects in occupations not associated with such exposure, and it turned out that there was no significant difference in SB levels in lymphocytes [18]. This was the case also in Rome, where traffic police were compared with office workers [19].…”

Section: Comet Assaymentioning

confidence: 99%

Atmospheric pollution in Kosovo is associated with increased DNA damage in the human population

Alija¹,

Asllani²,

Bajraktari³

et al. 2016

Biomonitoring

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Abstract:In order to assess DNA damage associated with exposure to environmental pollution in two polluted sites and one control site in Kosovo, whole blood samples were collected from volunteers in two polluted areas (Kastriot/ Obiliq - lignite-based power plants and lignite mines - and Drenas/Gllogovc - Ferronikeli smelting plant) as well as from Peja, representing an unpolluted area. White blood cells were isolated, and DNA damage was analyzed by the alkaline comet assay. Significantly higher levels of DNA damage (strand breaks) were found in white blood cells from subjects living in the polluted areas compared with residents of the unpolluted city, indicating a potential threat to human health.

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“…Briefly, the majority of the studies reveal a good correlation between exposure to environmental pollution and DNA damage, as well as associations with the results obtained from other biomarkers of exposure (36). For example, occupational exposure to carcinogenic PAHs reduced the ability of cells to repair damaged DNA (37), and the environmental exposure to PM10 from the emissions of an oil refinery increased DNA damage in lymphocytes obtained from exposed subjects (38). Moreover, in buccal epithelial cells from women chronically exposed to biomass smoke, there was a positive correlation between PM10 and PM2.5 indoor levels and DNA damage (39).…”

Section: Dna Adductsmentioning

confidence: 99%

Molecular biomarkers to assess health risks due to environmental contaminants exposure

Poblete-Naredo

Albores

2016

biomedica

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<p>Los biomarcadores, o bioindicadores, son herramientas métricas que, al compararse con los valores de referencia, permiten evaluar los riesgos y generar información objetiva que ayude a las autoridades a planificar estrategias efectivas, solucionar problemas de salud o ambientales y utilizar los recursos asignados de manera eficiente.<br />La evaluación de riesgos en salud es un ejercicio multidisciplinario y la biología molecular contribuye enormemente a estos estudios, dado que el genoma, el transcriptoma, el proteoma y el metaboloma pueden verse afectados por xenobióticos, lo que causa cambios cuya medición resulta útil en la adopción de decisiones. Dichos cambios pueden variar por la carga genética de cada individuo y su distribución polimorfa en las poblaciones expuestas se convierte en un factor esencial para una adecuada interpretación de resultados.<br />Por lo tanto, el objetivo del presente artículo fue hacer una revisión de los diferentes biomarcadores moleculares aplicables a la evaluación de riesgos para la salud provenientes de los contaminantes ambientales.</p>

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Influence of environmental exposure to PAHs on the susceptibility of lymphocytes to DNA-damage induction and on their repair capacity

Cited by 38 publications

References 22 publications

Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation

Genotoxic changes in peripheral lymphocytes after therapeutic exposure to crude coal tar and ultraviolet radiation

Atmospheric pollution in Kosovo is associated with increased DNA damage in the human population

Molecular biomarkers to assess health risks due to environmental contaminants exposure

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