“…On average, wheat grain has a protein content of 8-13% [6], which is contributed by water/salt-soluble albumins and globulins (15-20%) and gluten proteins (80-85%), the latter serving as storage proteins and endowing wheat products with the desired viscoelastic and gustatory properties [7][8][9]. Importantly, some wheat proteins can cause adverse inflammatory reactions in humans: (1) certain gluten peptide sequences trigger small intestinal and extraintestinal T cell activation and inflammation in patients with celiac disease [10,11]; (2) the family of wheat alpha-amylase/trypsin inhibitors (ATIs) stimulate intestinal innate immune cells via the activation of Toll-like receptor 4 and promote intestinal and extraintestinal inflammation [12][13][14][15][16][17]; (3) numerous wheat albumins and globulins, such as serpins, lipid transfer proteins, β-amylases and ATIs, as well as a few gluten proteins, can elicit respiratory and nutritional immediate allergic reactions [18][19][20][21]; and (4) a novel form of nutritional wheat allergy with an immediate intestinal, but a delayed clinical, reaction to wheat proteins is highly prevalent among patients with "irritable bowel syndrome" [22,23]. The first studies showed that wheat cultivars largely differ in the compositions of ATIs [24,25] or of the 33-mer α-gliadin peptide, a key immunogen for T cells in patients with celiac disease [26].…”