Influence of dissolved oxygen and shear conditions on clavulanic acid production by Streptomyces clavuligerus

Rosa, José César; Neto, Álvaro Baptista; Hokka, Carlos O.; Badino, Alberto Colli

doi:10.1007/s00449-004-0386-9

Cited by 62 publications

(64 citation statements)

References 19 publications

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“…The dissolved oxygen concentration remained between 54 and 75% of the saturation with air, showing that there was no oxygen limitation. The negative effect of low DO concentration on CephC and CA production has been recognized previously (Rollins et al, 1988a;Rosa et al, 2005;Yegneswaran et al, 1991). However, in the present work the production of CephC and CA was not affected, since high DO levels were always present.…”

Section: Fed-batch Cultures With Starch Feedingcontrasting

confidence: 50%

“…Previous work has shown that a high oxygen level is fundamental for the biosynthesis of CA and CephC (Rollins et al, 1988a(Rollins et al, , 1988b(Rollins et al, , 1991Rosa et al, 2005;Yegneswaran et al, 1988Yegneswaran et al, , 1991. The influence of carbon source and feeding conditions on production of both CA and CephC should therefore be studied under conditions that are not oxygen limited, which is possible using submerged bioreactor cultures.…”

Section: Introductionmentioning

confidence: 99%

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Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions

Bellão

Antonio

Araujo

et al. 2013

Braz. J. Chem. Eng.

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-The effect of carbon source and feeding conditions on the production of clavulanic acid (CA) and cephamycin C (CephC) by Streptomyces clavuligerus was investigated. In fed-batch experiments performed with glycerol feeding, production of CA exceeded that of CephC, and reached 1022 mg.L ) was obtained in fed-batch cultivation with glycerol feeding. In fed-batch experiments performed with starch feeding, the production of CephC was in general higher than that of CA. A dissociation index (DI) was used to identify feeding conditions that favored production of CephC relative to CA. In all cultures with glycerol, DI values were less than unity, indicating higher production of CA compared to CephC. Conversely, in cultures fed with starch, the DI values obtained were greater than unity. However, no carbon source or feeding condition was able to completely dissociate the production of CA from that of CephC.

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Section: Fed-batch Cultures With Starch Feedingcontrasting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions

Bellão

Antonio

Araujo

et al. 2013

Braz. J. Chem. Eng.

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“…The strain did not reduce nitrate to nitrite, and it hydrolyzed the starch ( Table 2). With auxiliary identification, some physiological appeals, such as degradation of starch, gelatin, and decline of nitrate, are also well-thought-out to determine the species' taxonomy of novel isolates strains as commended by Rosselló-Mora and Amann [40].…”

Section: Physiological and Biochemical Propertiesmentioning

confidence: 99%

MOLECULAR AND MORPHOLOGICAL IDENTIFICATION OF STREPTOMYCES SP. NRC-88 NOVA SPECIES AS Β-Lactamase INHIBITOR FOR PHARMACEUTICAL APPLICATION

Awad

Germoush

2017

Asian J Pharm Clin Res

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Objective: Clavulanic acid (CA) is a vital agent in the treatment of bacterial infections since it is a potent inhibitor of an extensive variety of β-lactamase enzymes. Its production from Streptomyces strains is fact of expanding clinical significance. This study aimed to isolate and characterize a promising Streptomyces (S) species strain which produced an effective β-lactamase inhibitor.Methods: Streptomyces sp. NRC-88 was isolated from an Egyptian soil sample. The phenotypic and phylogenetic examinations of 16S rRNA gene were investigated. The active metabolite of this strain (CA) was determined by particular synergistic bioassay, spectrophotometric assay, recognized by thin layer chromatography, and structure of the CA affirmed by high-performance liquid chromatography (HPLC) method. Results:A phylogenetic examination of the 16S rRNA gene sequence of the NRC-88 strain consistent with conventional taxonomy was carried out, and confirmed that the strain NRC-88 was most similar to S. aburaviensis S-66 (99%). The active metabolite of this strain (CA) was determined by different methods and confirmed the structure of the CA by the HPLC method. It produced up to 87 mg/l in a specific CA production medium. Conclusion:A new species of Streptomyces sp. NRC-88 isolated and recognized by phenotypic and genotypic proof. This strain suggested the name, Streptomyces sp. NRC-88 (accession number KM014489). It was able to produce CA as the β-lactamase inhibitor.

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“…As Tabelas 1 e 2 apresentam a composição do meio de reativação, crescimento e produção, respectivamente (ROSA et al, 2005;TEODORO et al, 2006 …”

Section: Meio De Culturaunclassified

Determinação Do Ph E Temperatura Ótimos Para a Produtividade Do Ácido Clavulânico Utilizando O Streptomyces Clavuligerus Atcc 27064

Pinelli¹,

Dias²

2017

MUNDI ETG

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Determinação do pH e temperatura ótimos para a produtividade do ácido clavulânico utilizando o Streptomyces clavuligerus ATCC 27064Determination of pH and temperature optimum on the productivity of clavulanic acid using Streptomyces clavuligerus ATCC 27064Juliana Junqueira Pinelli 1 Sandra de Cássia Dias 2 Resumo: O ácido clavulânico (AC) é um metabólito secundário produzido pela bactéria GramPositiva Streptomyces clavuligerus. É um composto de suma importância, principalmente para a indústria farmacêutica, pois possui ação antibacteriana. É utilizado em associação com outros antibióticos, como a amoxicilina. Sua produção ocorre por via fermentativa. O pH e a temperatura do meio são parâmetros relevantes que podem comprometer a produção deste metabólito. O presente trabalho teve como objetivo avaliar a influência da temperatura e do pH na produção de ácido clavulânico a partir de Streptomyces clavuligerus ATCC 27064. Para isso, um número mínimo de experimentos foi desenhado com diferentes valores de pH e temperatura utilizando o planejamento experimental estatístico, delineamento composto central rotacional, DCCR, tendo como variáveis pH e temperatura e como resposta a produtividade do ácido clavulânico. Amostras foram retiradas a cada 12 horas e a produção de ácido clavulânico determinada por método espectrofotométrico. O cultivo realizado em pH 6,7 e temperatura 27ºC apresentou maior produtividade do ácido clavulânico. Palavras-chave:Delineamento composto central rotacional. Antibiótico. Metodologia de superfície de resposta. Bioprocessos Abstract: Clavulanic acid (CA) is a secondary metabolite produced by the Gram-Positive bacteria Streptomyces clavuligerus. It is a compound of great importance, mainly for the pharmaceutical industry, because it has antibacterial activity. It is used in combination with other antibiotics such as amoxicillin. Its production occurs by fermentation. The pH and temperature of the medium are relevant parameters that can compromise the production of this metabolite. The aim of this study was to evaluate the temperature and pH influence on the clavulanic acid productivity by Streptomyces clavuligerus ATCC 27064. For this, a minimum number of experiments were designed with different values of pH and temperature using statistical experimental design, central composite rotational design (CCRD) having as variables pH and temperature and as response the clavulanic acid productivity. Samples were collected every 12 hours and the clavulanic acid production was determined by spectrophotometric method. The cultivation carried out at pH 6.7 and temperature 27ºC showed the highest clavulanic acid productivity.

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Influence of dissolved oxygen and shear conditions on clavulanic acid production by Streptomyces clavuligerus

Cited by 62 publications

References 19 publications

Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions

Production of clavulanic acid and cephamycin C by Streptomyces clavuligerus under different fed-batch conditions

MOLECULAR AND MORPHOLOGICAL IDENTIFICATION OF STREPTOMYCES SP. NRC-88 NOVA SPECIES AS Β-Lactamase INHIBITOR FOR PHARMACEUTICAL APPLICATION

Determinação Do Ph E Temperatura Ótimos Para a Produtividade Do Ácido Clavulânico Utilizando O Streptomyces Clavuligerus Atcc 27064

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