Influence of CAD/CAM all-ceramic materials on cell viability, migration ability and adenylate kinase release of human gingival fibroblasts and oral keratinocytes

Pabst, Andreas; Grassmann, L; Weyhrauch, Michael; Brüllmann, Dan; Ziebart, Thomas; Scheller, Herbert; Lehmann, Karl Martin

doi:10.1007/s00784-013-1098-9

Cited by 27 publications

(40 citation statements)

References 29 publications

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“…Pabst et al investigated the influence of four CAD/CAM all‐ceramic (e.max CAD LT, e.max CAD HT, Empress CAD, Mark II) materials on cell viability. According to their MTT assay results for human gingival fibroblasts in all material groups, cell viability showed no significant decrease at all time periods (3, 6, 9, and 12 days) . In the results of this study, only the Empress CAD group presented a significant decrease in cell viability for the 7 th day of the test period.…”

Section: Discussioncontrasting

confidence: 71%

Effects of New Generation All‐Ceramic and Provisional Materials on Fibroblast Cells

Atay

Gürdal

Çetintaş

et al. 2018

Journal of Prosthodontics

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Section: Discussioncontrasting

confidence: 71%

Effects of New Generation All‐Ceramic and Provisional Materials on Fibroblast Cells

Atay

Gürdal

Çetintaş

et al. 2018

Journal of Prosthodontics

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“…Actually, titanium is a gold-standard material in the field of dental implant prosthodontics mainly due to its high corrosion resistance, low toxicity, very low allergenic potential and good biocompatibility properties (Jorge et al 2013). Regarding lithium disilicate glassceramic, HGF behavior on this material has been poorly studied (Brackett et al 2008;Pabst et al 2014;Tetè et al 2014). The present results showed a good cellular attachment on lithium disilicate glass-ceramic but a slower proliferation than on titanium.…”

Section: Discussionmentioning

confidence: 99%

“…To author's knowledge there is no study comparing the attachment and proliferation of HGFs on titanium and on lithium disilicate glass-ceramic. Tetè et al (2014) reported that lithium disilicate glass-ceramic shows a lower cytocompatibility than zirconia and other authors observed that this material shows cytotoxic effects (Brackett et al 2008;Pabst et al 2014).…”

Section: Discussionmentioning

confidence: 99%

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

et al. 2016

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In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to study cell behavior on rigid and flexible/permeable biomaterials elaborating two specific insert-based systems (IBS-R and IBS-F respectively). IBS-R was designed as a specific cylindrical polytetrafluoroethylene (PTFE) system to evaluate attachment, proliferation and morphology of human gingival fibroblasts (HGFs) on grade V titanium and lithium disilicate glassceramic discs characteristics of dental prostheses. The number of cells, their covering on discs and their morphology were determined from MTS assays and microscopic fluorescent images after 24, 48 and 72 h. IBS-F was developed as a two components system to study HGFs behavior on guided bone regeneration polyester membranes. The viability and the membrane barrier effect were evaluated by metabolic MTS assays and by scanning electron microscopy. IBS-R and IBS-F were shown to promote (1) easy and rapid handling; (2) cell retention on biomaterial surface; (3) accurate evaluation of the cellular proliferation, spreading and viability; (4) use of non-toxic material. Moreover IBS-F allowed the study of the cell migration through degradable membranes, with an access to both faces of the biomaterial and to the bottom of culture wells for medium changing.

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“…Among the dental ceramics, zirconia was introduced with the purpose of meeting the requirements of aesthetics and resistance, and its use has been widely disseminated in conjunction with computer-aided design/ computer-assisted manufacturing (CAD/CAM) systems (4). Resin-matrix ceramics or indirect composites for CAD/CAM applications can be classified according to their microstructure into dispersed fillers (zirconia-silica nanofillers and organic matrix) and polymer-infiltratedceramic-networks (PICNs), based on the infiltration of a pre-sintered glass-ceramic scaffold with a polymeric material (5).…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, the in vitro cellular responses to several types of dental ceramics in use have not always been favorable (11). The CAD/CAMmilled, all-ceramic materials used in implant abutments come in close contact with oral soft tissues such as the keratinized marginal gingiva, which may raise concerns about the safety and biocompatibility of these products (4). According to Tassin et al (12), significant cytotoxicity may be observed for conventional composites, while PICNs such as Vita Enamic proved to be very biocompatible during in vitro assays.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Analysis of the Cytotoxicity of Indirect Restorative Materials

et al. 2018

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This study aimed to compare the cytotoxicity of the Vita AC12, Lava Ultimate, Vita Enamic and InSync indirect restorative materials. Extracts of each material were prepared by incubation for 1, 7 and 40 days, with daily washing. Human gingival fibroblasts were exposed to the extracts, and cell viability was evaluated by sequential assessment of mitochondrial activity (XTT), membrane integrity (NRU) and cell density (CVDE). Extracts of polystyrene beads and latex fragments were used as negative and positive controls, respectively. Differences between groups and experimental times were evaluated by analysis of variance. At the 24 h extraction, significant differences between the control and both Vita AC-12 and InSync were observed in the XTT assay (p<0.05), and between the control and both Enamic and Lava Ultimate, in the CVDE assay (p<0.05). AC12, Lava Ultimate, and InSync presented significantly lower cell viability than Enamic and the control group, in the NRU assay (p<0.05). The Vita Enamic and Lava Ultimate hybrid ceramic-like materials presented better biocompatibility at the 24 h extraction time point than the AC12 and InSync ceramic materials. However, a simulation of the removal of toxic components by biological fluids, conducted by using longer extraction times and daily washing, led to the absence of cytotoxicity in all the tested restorative materials. These findings can be viewed as positive for the clinical indication of these restorative materials, considering their contact with adjacent soft tissues for extended periods of time.

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Influence of CAD/CAM all-ceramic materials on cell viability, migration ability and adenylate kinase release of human gingival fibroblasts and oral keratinocytes

Abstract: CAD/CAM all-ceramic materials can influence oral cell lines responsible for soft tissue creation which may affect the esthetic outcome.

Cited by 27 publications

References 29 publications

Effects of New Generation All‐Ceramic and Provisional Materials on Fibroblast Cells

Effects of New Generation All‐Ceramic and Provisional Materials on Fibroblast Cells

A new method using insert-based systems (IBS) to improve cell behavior study on flexible and rigid biomaterials

In Vitro Analysis of the Cytotoxicity of Indirect Restorative Materials

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