Influence of Artesunate Combinative Therapy Co-Administration with Rutin on Inflammatory Cytokines and Immunoglobulins in Plasmodium berghei-Infected Mice

Olanlokun, John Oludele; Balogun, Adisa Abayomi; Olorunsogo, Olufunso O.

doi:10.1645/20-87

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…This corroborates with a study carried out by Emeka et al which showed that the combinatory effect of Nigella sativa formulated feed and CQ generated parasitemia clearance (i.e., 100% parasitemia suppression) in the infected mice (Emeka et al, 2014). Considering the central targets of plasmodium parasites such as the liver, blood tissues (Olanlokun et al, 2021),…”

Section: Moreover Death Is the Terminal Complication Of Plasmodiumsupporting

confidence: 89%

“…This corroborates with a study carried out by Emeka et al which showed that the combinatory effect of Nigella sativa formulated feed and CQ generated parasitemia clearance (that is, 100% parasitemia suppression) in the infected mice (Emeka et al, 2014). Considering the central targets of plasmodium parasites such as the liver, spleen and blood tissues, we assessed the effect of N.sativa on the hematological parameters of the infected mice which include the packed cell volume (PCV), also known as the haematocrit, heamoglobin and the red blood cells (RBCs) (Olanlokun et al, 2021). A signi cant reduction in the total number of red blood cells (RBCs) in the P. berghei -infected mice observed in this study revealed detrimental signs of severe anaemia which is a core clinical indication of complicated malaria in patients (Keating et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Haemoglobin is the iron-containing oxygen-transport metalloprotein present in RBCs of animals and its purpose is to transport oxygen to tissues for the oxidation of ingested food in order to liberate energy for normal functioning of the biological system. A particular study revealed signi cant reduction in the packed cell volume, hemoglobin and red blood cells in the rats' model infected with P. berghei ANKA strain (Olanlokun et al, 2021). The packed cell volume (PCV) is the quanti cation of the proportion of blood that is composed of cells and denotes the capacity of blood to transport oxygen and nutrients.…”

Section: Discussionmentioning

confidence: 99%

“…This nding corroborates previous studies which revealed in the signi cant reduction in the PCV of Plasmodium parasites-infected rabbit and mice. (Olanlokun et al, 2021;Chijioke et al, 2018). All hematological indices (PCV, RBCs and heamoglobin) carried out in this study were reinstated following treatment with black seed.…”

Section: Discussionmentioning

confidence: 99%

“…Intriguingly, dietary supplementation with black seed signi cantly elevated GPx, GST and GSH levels. Glutathione-Stransferase (GST), a phase II detoxi cation enzyme and reduced glutathione are core players in ROS and RNS detoxi cation and a relevant mediator in oxidative stress and in ammatory response in malaria parasites (Olanlokun et al, 2021). The trophozoite phase of the malarial parasite in the erythrocytes shows a potent reduced glutathione (GSH) metabolism.…”

Section: Discussionmentioning

confidence: 99%

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Effect of black seeds ( Nigella sativa ) on inflammatory and immunomodulatory markers in Plasmodium berghei ‐infected mice

Ojueromi

Oboh

Ademosun

2022

Journal of Food Biochemistry

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N.sativa, a core dietary supplement and food additive in folklore is one of the most broadly studied seed plants in the global nutraceutical sector. Malaria infection impairs the ability of principal cells of the immune system to trigger an e cient in ammatory and immune response. Ninety-six mice weighing (20-25g) were grouped into twelve consisting of eight animals each. The mice were infected with standard inoculum of the strain NK65 P.berghei (chloroquine sensitive) and the percentage parasitemia suppression were evaluated. The individual effect of black seed supplemented diet and its combinatory effect with chloroquine (CQ) were investigated on the glutathione peroxidase(GPx), reduced glutathione(GSH), glutathione-S-transferase (GST), serum immunoglobulins (IgG and IgM), and the hematological parameters (haemoglobin, packed cell volume and red blood cell count) in P.berghei infected mice. The in ammatory cytokines; Tumor necrosis factor (TNF-α), C-reactive protein (CRP), Interleukin (IL-6 and IL-10) as well as IgG and IgM were assayed in the serum. The mice temperature and behavioural changes were observed. Infected mice treated with the dietary supplementation of black seed with a percentage inclusion (2.5%, 5% and 10%) showed signi cantly decreased parasitemia levels (p<0.05) compared with the untreated mice. The result demonstrated a signi cant suppression in the proin ammatory cytokines (TNF-α, CRP, IL-6) levels and a notable elevation in the anti-in ammatory cytokine (IL-10), antioxidant markers as well as the immunoglobulin levels of the P.berghei-infected mice treated with black seed. The study revealed that black seed enhanced host antioxidant status, modulated in ammatory and immune response by regulating some in ammatory cytokines and immunomodulatory mediators. Pratical ApplicationBlack seed (N.sativa) has been a dietary supplement and natural remedy for many ailments for many centuries. In ammatory and immune diseases are the most notable cause of mortality in the world and more than fty percent of deaths have been attributed to it. However, there's paucity of information on the effect of N.sativa on anti-in ammatory and immunomodulatory ability of N.sativa. The result suggests that N.sativa induced antioxidant, anti-in ammatory and immunomodulatory effect in P.bergheiinfected mice via the participation of glutathione antioxidant system, serum antibodies and some in ammatory cytokines.2.7 Grouping of Experimental Animals. Group 1: Normal control (Mice fed with basal diet) Group 2: Plasmodium berghei-infected mice (PBIM) Group 3: PBIM + chloroquine (CQ) (10 mg/kg)

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Section: Moreover Death Is the Terminal Complication Of Plasmodiumsupporting

confidence: 89%