Influence of adding fibrolytic enzymes on the ruminal fermentation of date palm by-products

Abstract

This study was conducted in order to assess the influence of four doses (0, 0.5, 1, and 2 mg (g dry matter)-normal1 of commercial fibrolytic enzymes (MAXFIBER-Inormal®, SHAUMANN GmbH, Wahlstedt, Germany) on in vitro fermentation of date palm (Phoenix dactylifera) by-products: date kernels, wasted dates, floral stems, and palm fronds. Rumen contents were obtained from two non-lactating Holstein cows. Enzyme supplementation to by-products was carried out 12 h prior to incubation.Compared to the control, the enzymatic supplementation quadratically increased the extent but not the gas production rate of date kernel fermentation. Indeed, the potential gas production increased notably by 14.8 % with the lowest enzymes dose following recorded gas production after 48, 72, and 96 h of incubation. The estimated organic-matter digestibility, metabolisable energy, and total volatile fatty acids in the incubation fluid tended to be increased with the lowest dose by 7.8 %, 8.4 %, and 13.9 % respectively. For the wasted dates, this feed additive tended to linearly increase the gas production rate of fermentation with the highest dose. On the other hand, this supplementation had no effect on the ruminal fermentation of the floral stems and palm fronds. The exogenous fibrolytic enzymes were more effective on fibrous but not on lignified date palm by-products.

In vitro study on the effects of exogenic fibrolytic enzymes produced from <i>Trichoderma longibrachiatum</i> on ruminal degradation of olive mill waste

“…For olive leaves, the increased dose of EFE linearly decreased the hemicellulose, increased dry matter solubility, and reduced sugars. A similar result was found on rich tannin feed like almond hulls (Abid et al, 2019b). This modification of chemical composition could modify the microorganisms in the ru-men and microbial colonization.…”

“…The biological treatments are more effective than the chemical treat-ments due to their greater substrate specificity and their lower pollution effects (Misra et al, 2007). The treatment of fibrous feed by biological additives like exogenic fibrolytic enzymes (EFE) improved the nutritional value, growth performance, milk production, and economic returns of ruminants (Mohamed et al, 2013;Abid et al, 2019bAbid et al, , 2020. Therefore, the objective of this research is to valorize olive mill waste by supplementing ruminant nutrition with EFE.…”

The effects of compound treatment of Aspergillus oryzae and fibrolytic enzyme on in vitro degradation, gas production and fermentative profile of maize silage and sugarcane silage

“…Exogenous fibrolytic enzyme increases the dry matter (DM) and neutral detergent fibre (NDF) in vitro degradation (Gandra et al ., 2017; Zayed et al ., 2020), fermentative kinetics (Elghandour et al ., 2016) and beef cattle average daily gain (Tirado-González et al ., 2018). However, the efficiency of exogenous enzyme is highly variable (Meale et al ., 2014; Abid et al ., 2019). The beneficial impact of the exogenous fibrolytic enzymes on fibre digestibility depends on several factors, such as the basal diet composition, enzymatic preparation, methods of application and doses (Mendoza et al ., 2014; Elghandour et al ., 2016), ruminal retention time and pH, which affects the ruminal activity and enzymatic stability (Meale et al ., 2014).…”

Milk Production and Feeding Behavior of Lactating Cows Supplemented With a Dry Fungus Fermentation Product That Expresses Residual Fibrolytic Enzyme Activity / Produção De Leite E Comportamento Alimentar De Vacas Em Lactação Suplementadas Com Um Produto De Fermentação De Fungo Seco Que Expressa Atividade De Enzima Fibrolítica Residual

“…This possible increase in energy availability caused by supplementation may have caused an increase in yield milk, a fact observed in the GS of the current study. Ratifying this speculation Oh et al (2019) and Abid et al (2019) observed an increase in AGCC synthesis with EFE supplementation, also suggesting that the occurrence of this fact is due to greater rumen enzymatic activity.…”