Influence of a high-glucose diet on the sensitivity of Caenorhabditis elegans towards Escherichia coli and Staphylococcus aureus strains

Lavigne, Jean‐Philippe; Audibert, Sylvain; Molinari, Nicolas; O’Callaghan, David; Keriel, Anne

doi:10.1016/j.micinf.2013.04.006

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…1C). Consistent with our study, another recent report also shows that glucose has no additive effect on killing, sometimes even extends the survival of C. elegans that are infected with certain pathogens [41]. These results suggested that S. typhimurium and glucose may shorten lifespan through a common pathway.…”

Section: Discussionsupporting

confidence: 92%

Glucose negatively affects Nrf2/SKN-1-mediated innate immunity in C. elegans

Chen

Chenzhao

et al. 2018

Aging

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High glucose levels negatively affect immune response. However, the underlying mechanisms are not well understood. Upon infection, the round worm C. elegans induces multiple gene transcription programs, including the Nrf2/SKN-1-mediated detoxification program, to activate the innate immunity. In this study, we find that high glucose conditions inhibit the SKN-1-mediated immune response to Salmonella typhimurium, exacerbate the infection and greatly decrease survival. The effect of glucose shows specificity to SKN-1 pathway, as UPRmit and UPRER that are known to be induced by infection, are not affected. Hyper-activation of SKN-1 by wdr-23 RNAi restores partly the immune response and increases the survival rate in response to S. typhimurium. In all, our study reveals a molecular pathway responsible for glucose’s negative effect on innate immunity, which could help to better understand diseases associated with hyperglycemia.

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Section: Discussionsupporting

confidence: 92%

Glucose negatively affects Nrf2/SKN-1-mediated innate immunity in C. elegans

Chen

Chenzhao

et al. 2018

Aging

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“…No significant difference was noted in the fraction of nematodes on the bacterial lawn between the different associations studied (Tables 1–3, Tables S1–S3). We also measured the bacterial load in the intestine of nematodes at 72 h post infection (Lavigne et al, 2013). We found that all bacteria tested alone or in association can colonize and survive in the C. elegans intestine (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

“…Around 30 L4 stage nematodes were deposed in the center of the bacteria lawn. To establish the occupancy assays, the number of nematodes inside or outside each lawn was counted after overnight incubation as previously published (Lavigne et al, 2013). The results were presented in percent occupancy (number of worms in the bacterial lawn on the total number of C. elegans ).…”

Section: Methodsmentioning

confidence: 99%

Decrease of Staphylococcus aureus Virulence by Helcococcus kunzii in a Caenorhabditis elegans Model

Essebe

Visvikis

Fines-Guyon

et al. 2017

Front. Cell. Infect. Microbiol.

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Social bacterial interactions are considered essential in numerous infectious diseases, particularly in wounds. Foot ulcers are a common complication in diabetic patients and these ulcers become frequently infected. This infection is usually polymicrobial promoting cell-to-cell communications. Staphylococcus aureus is the most prevalent pathogen isolated. Its association with Helcococcus kunzii, commensal Gram-positive cocci, is frequently described. The aim of this study was to assess the impact of co-infection on virulence of both H. kunzii and S. aureus strains in a Caenorhabditis elegans model. To study the host response, qRT-PCRs targeting host defense genes were performed. We observed that H. kunzii strains harbored a very low (LT50: 5.7 days ± 0.4) or an absence of virulence (LT50: 6.9 days ± 0.5). In contrast, S. aureus strains (LT50: 2.9 days ± 0.4) were significantly more virulent than all H. kunzii (P < 0.001). When H. kunzii and S. aureus strains were associated, H. kunzii significantly reduced the virulence of the S. aureus strain in nematodes (LT50 between 4.4 and 5.2 days; P < 0.001). To evaluate the impact of these strains on host response, transcriptomic analysis showed that the ingestion of S. aureus led to a strong induction of defense genes (lys-5, sodh-1, and cyp-37B1) while H. kunzii did not. No statistical difference of host response genes expression was observed when C. elegans were infected with either S. aureus alone or with S. aureus + H. kunzii. Moreover, two well-characterized virulence factors (hla and agr) present in S. aureus were down-regulated when S. aureus were co-infected with H. kunzii. This study showed that H. kunzii decreased the virulence of S. aureus without modifying directly the host defense response. Factor(s) produced by this bacterium modulating the staphylococci virulence must be investigated.

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Effects of microwave radiation on micro-organisms in selected materials from healthcare waste

Mahdi

Gomes

2018

Int. J. Environ. Sci. Technol.

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Influence of a high-glucose diet on the sensitivity of Caenorhabditis elegans towards Escherichia coli and Staphylococcus aureus strains

Cited by 7 publications

References 44 publications

Glucose negatively affects Nrf2/SKN-1-mediated innate immunity in C. elegans

Glucose negatively affects Nrf2/SKN-1-mediated innate immunity in C. elegans

Decrease of Staphylococcus aureus Virulence by Helcococcus kunzii in a Caenorhabditis elegans Model

Effects of microwave radiation on micro-organisms in selected materials from healthcare waste

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