Influence of a facile pretreatment process on lipid extraction from <i>Nannochloropsis</i> sp. through an enzymatic hydrolysis reaction

Chen, Qingtai; Liu, Dong; Wu, Chongchong; Xu, Airong; Xia, Wei; Wang, Zhaowen; Wen, Fusheng; Yu, Daoyong

doi:10.1039/c7ra11483d

Cited by 16 publications

(4 citation statements)

References 44 publications

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“…One of the key features of microalgae is the rigidity of its cell wall, while damage to their cell wall permits further CO 2 -SF to reach the selective compound. There are several cell disruption methods, such as mechanical, physical, chemical, and enzymatic approaches [ 3 , 19 , 43 , 44 , 45 ]. Lee et al [ 17 ] reported a study to compare different microalgal biomass pretreatment methods and suggested that mechanical disruption methods be considered as highly energy efficient approaches when conducted under laboratory conditions.…”

Section: Resultsmentioning

confidence: 99%

Eicosapentaenoic Acid Extraction from Nannochloropsis gaditana using Carbon Dioxide at Supercritical Conditions

et al. 2019

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This research shows that carbon dioxide supercritical fluid (CO2-SF) is an emerging technology for the extraction of high interest compounds for applications in the manufacturing of pharmaceuticals, nutraceuticals, and cosmetics from microalgae. The purpose of this study is to recover fatty acids (FAs) and, more precisely, eicosapentaenoic acid (EPA) from Nannochloropsis gaditana biomass by CO2-SF extraction. In the paper, the effect of mechanical pre-treatment was evaluated with the aim of increasing FAs recovery. Extraction was performed at a pressure range of 250–550 bars and a CO2 flow rate of 7.24 and 14.48 g/min, while temperature was fixed at 50 or 65 °C. The effect of these parameters on the extraction yield was assessed at each extraction cycle, 20 min each, for a total extraction time of 100 min. Furthermore, the effect of biomass loading on EPA recovery was evaluated. The highest EPA extraction yield, i.e., 11.50 mg/g, corresponding to 27.4% EPA recovery, was obtained at 65 °C and 250 bars with a CO2 flow rate of 7.24 g/min and 1.0 g biomass loading. The increased CO2 flow rate from 7.24 to 14.48 g/min enhanced the cumulative EPA recovery at 250 bars. The purity of EPA could be improved by biomass loading of 2.01 g, even if recovery was reduced.

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Section: Resultsmentioning

confidence: 99%

Eicosapentaenoic Acid Extraction from Nannochloropsis gaditana using Carbon Dioxide at Supercritical Conditions

et al. 2019

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“…It was also reported that during the bead miller pre-treatment the appropriate mixing of biomass and alumina (1:1 w / w ) was important to increase the lutein extraction [19]. In addition, several reports suggest that pretreatment of microalgae biomass at optimum condition is essential to enhance the extraction of intracellular compounds [28,29,30,31]. In this work, the effective pretreatment of S. almeriensis biomass was achieved at 400 rpm for 5 min with 0.4 DE/biomass ratios to assist with the extraction of intracellular molecules.…”

Section: Resultsmentioning

confidence: 99%

Supercritical Fluid Extraction of Lutein from Scenedesmus almeriensis

et al. 2019

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Lutein has several benefits for human health, playing an important role in the prevention of age-related macular degeneration (AMD), cataracts, amelioration of the first stages of atherosclerosis, and some types of cancer. In this work, the Scenedesmus almeriensis microalga was used as a natural source for the supercritical fluid (SF) extraction of lutein. For this purpose, the optimization of the main parameters affecting the extraction, such as biomass pre-treatment, temperature, pressure, and carbon dioxide (CO2) flow rate, was performed. In the first stage, the effect of mechanical pre-treatment (diatomaceous earth (DE) and biomass mixing in the range 0.25–1 DE/biomass; grinding speed varying between 0 and 600 rpm, and pre-treatment time changing from 2.5 to 10 min), was evaluated on lutein extraction efficiency. In the second stage, the influence of SF-CO2 extraction parameters such as pressure (25–55 MPa), temperature (50 and 65 °C), and CO2 flow rate (7.24 and 14.48 g/min) on lutein recovery and purity was investigated. The results demonstrated that by increasing temperature, pressure, and CO2 flow rate lutein recovery and purity were improved. The maximum lutein recovery (~98%) with purity of ~34% was achieved operating at 65 °C and 55 MPa with a CO2 flow rate of 14.48 g/min. Therefore, optimum conditions could be useful in food industries for lutein supplementation in food products.

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“…Cellulase, pectinase and lysozyme were used together for lipid extraction from Nannochloropsis sp. with a 22% recovery yield (Chen et al, 2017). In some cases, the cellulase-protease combination performed poorly.…”

Section: Enzymatic Methodsmentioning

confidence: 99%

Current and novel approaches to downstream processing of microalgae: A review

Nitsos

Filali

Taidi

et al. 2020

Biotechnology Advances

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HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

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Influence of a facile pretreatment process on lipid extraction from Nannochloropsis sp. through an enzymatic hydrolysis reaction

Abstract: A wall-breaking technology for algal cell composed of swelling by weak alkali and decomposition by enzyme was developed.

Cited by 16 publications

References 44 publications

Eicosapentaenoic Acid Extraction from Nannochloropsis gaditana using Carbon Dioxide at Supercritical Conditions

Eicosapentaenoic Acid Extraction from Nannochloropsis gaditana using Carbon Dioxide at Supercritical Conditions

Supercritical Fluid Extraction of Lutein from Scenedesmus almeriensis

Current and novel approaches to downstream processing of microalgae: A review

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