Influence of αS1-casein and κ-casein polymorphism on the curd yield of Italian Mediterranean buffalo (Bubalus bubalis L.) milk

Zicarelli, L.; Palo, R. Di; Napolano, R.; Tonhati, Humberto; Carlo, Esterina De; Gagliardi, R.; Luccia, Aldo Di; Gatta, Barbara la

doi:10.1016/j.idairyj.2019.104559

Cited by 6 publications

(9 citation statements)

References 23 publications

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“…Genotyping data indicated that minor allele frequencies were 0.425 for the CSN1S1 A and 0.306 for the CSN3 X2 (Table 1). Our results agree with the data produced by Bonfatti et al (2012) and Zicarelli et al (2020). However, in this respect, it must be underlined that in the two quoted papers (Bonfatti et al 2012;Zicarelli et al 2020) the allele nomenclature for both loci was reversed in comparison to the original one (Mitra et al 1998;Chianese et al 2009) that we considered as reference.…”

Section: Resultssupporting

confidence: 89%

“…This is mainly due to the rheological characteristics of its milk, known to have higher protein and fat content compared to other ruminant's milk. These properties make it particularly suitable for the cheesemaking with a higher yield production (Zicarelli 2004;Zicarelli et al 2020). Despite the potential economic impact on the dairy industry, increasing buffalo milk production with optimal percentages of fats and proteins through an adequate genetic selection process still reveal delays.…”

Section: Resultsmentioning

confidence: 99%

“…Despite the potential economic impact on the dairy industry, increasing buffalo milk production with optimal percentages of fats and proteins through an adequate genetic selection process still reveal delays. This is also due to the lack of fast methods for the discrimination of favourable alleles for more profitable breeding strategies, such as the A/B and X1/X2 alleles at as1and j-casein genes, respectively (Bonfatti et al 2012;Bonfatti et al 2013;Cosenza et al 2015;Zicarelli et al 2020).…”

Section: Resultsmentioning

confidence: 99%

“…To date, RP-HPLC of milk protein and/or DNA sequencing were the most used methods for genotype determination at CSN1S1 and CSN3 genes (Chianese et al 2009;Bonfatti et al 2012;Bonfatti et al 2013;Zicarelli et al 2020). However, both methods require several working steps and have limitations.…”

Section: Resultsmentioning

confidence: 99%

“…In addition to shortest RCT and K 20 , genotype AA-X1X2 also revealed the largest curd firmness (A 30 ). Furthermore, recently Zicarelli et al (2020) demonstrated that milk carrying CSN1S1 Ã B and CSN3 Ã X1 alleles resulted in a greater curd yield.…”

Section: Introductionmentioning

confidence: 99%

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A novel duplex ACRS-PCR for composite CSN1S1–CSN3 genotype discrimination in domestic buffalo

Pauciullo

Martorello

Carku

et al. 2021

Italian Journal of Animal Science

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This short communication aimed to develop a fast and straightforward method for the simultaneous discrimination in buffaloes of the alleles CSN1S1 A/B and CSN3 X1/X2 using a single protocol. DNA was isolated from 219 individual blood samples. A duplex artificially created restriction site (ACRS-PCR) was accomplished using two pairs of primers generating 86 bp (CSN1S1) and 160 bp (CSN3). Amplicons were contemporary digested by MboI and HinfI for the identification of genotypes. The double simultaneous amplification and digestion proved to be effective for allele identification. The method resulted particularly quick due to the small PCR amplicons and fast digest enzymes that allowed both a rapid amplification (about 1 h and 40 min) and digestion in 10 min. Population analysis indicated that minor allele frequencies were CSN1S1 A (0.425) and CSN3 X2 (0.306). Linkage disequilibrium (LD) showed r 2 ¼0.46, and haplotype analysis revealed all four possible combinations with higher frequency for the CSN1S1 B-CSN3 X1 (0.553). Considering the tremendous economic impact of the CSN1S1-CSN3 variants on the dairy production in buffalo, this method, applicable immediately after the birth from any DNA source, may speed up the selection of sires and dams' lines with more favourable genotypes. HIGHLIGHTSCSN1S1-CSN3 composite genotype influences dairy performances. Duplex ACRS-PCR identifies the CSN1S1-CSN3 composite genotype in a single protocol. The duplex ACRS-PCR method may speed up the selection of lines with more favourable genotypes.

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Influence of αS1-casein and κ-casein polymorphism on the curd yield of Italian Mediterranean buffalo (Bubalus bubalis L.) milk

Cited by 6 publications

References 23 publications

A novel duplex ACRS-PCR for composite CSN1S1–CSN3 genotype discrimination in domestic buffalo

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