Inflorescence Development and the Role of LsFT in Regulating Bolting in Lettuce (Lactuca sativa L.)

Chen, Zijing; Han, Yingyan; Ning, Kang; Ding, Yunyu; Zhao, Wensheng; Yan, Shuangshuang; Luo, Chen; Jiang, Xiaotang; Ge, Danfeng; Liu, Renyi; Wang, Qian; Zhang, Xiaolan

doi:10.3389/fpls.2017.02248

Cited by 51 publications

(66 citation statements)

References 49 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Once it bolts and flowers, the plant loses its economic value. To gain insight into the floral transition in lettuce, we observed the morphological features of the bolting-sensitive line S39 in our recent study (Chen et al, 2017). Four critical developmental stages were identified in S39: early vegetative stage B0 [20 days after planting (DAP)], late vegetative stage B1 (28 DAP), vegetative to reproductive stage B2 (35 DAP), and reproductive stage B3 (45 DAP).…”

Section: Lcm-seq Of Shoot Tissues In the Bolting-sensitive Line S39 Dmentioning

confidence: 99%

“…FT is the direct target gene in floral inductive pathway and SOC1 is a downstream gene for FT-FD dimer in Arabidopsis (Lee et al, 2000;Wigge et al, 2005). To clarify whether heat treatment induces FT to activate SOC1 expression, we detected the expression of LsSOC1 in three LsFT-RNAi lines, which were raised in our previous study, under mock treatment and heat treatment (Chen et al, 2017). The transcript level of LsSOC1 in the LsFT-RNAi lines increased under heat treatment compared with that under mock treatment ( Figure S5).…”

Section: Functional Characterization Of Lssoc1mentioning

confidence: 99%

“…We recently studied the morphology of the shoot apical meristem (SAM) during time course development in lettuce (Chen et al, 2017). The molecular mechanism underlying the floral transition in the lettuce SAM has not been thoroughly studied.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

LCM‐seq reveals the crucial role of LsSOC1 in heat‐promoted bolting of lettuce (Lactuca sativa L.)

Chen

Zhao

et al. 2018

The Plant Journal

Self Cite

View full text Add to dashboard Cite

Lettuce (Lactuca sativa L.) is one of the most economically important vegetables. The floral transition in lettuce is accelerated under high temperatures, which can significantly decrease yields. However, the molecular mechanism underlying the floral transition in lettuce is poorly known. Using laser capture microdissection coupled with RNA sequencing, we isolated shoot apical meristem cells from the bolting-sensitive lettuce line S39 at four critical stages of development. Subsequently, we screened specifically for the flowering-related gene LsSOC1 during the floral transition through comparative transcriptomic analysis. Molecular biology, developmental biology, and biochemical tools were combined to investigate the biological function of LsSOC1 in lettuce. LsSOC1 knockdown by RNA interference resulted in a significant delay in the timing of bolting and insensitivity to high temperature, which indicated that LsSOC1 functions as an activator during heat-promoted bolting in lettuce. We determined that two heat shock transcription factors, HsfA1e and HsfA4c, bound to the promoter of LsSOC1 to confirm that LsSOC1 played an important role in heat-promoted bolting. This study indicates that LsSOC1 plays a crucial role in the heat-promoted bolting process in lettuce. Further investigation of LsSOC1 may be useful for clarification of the bolting mechanism in lettuce.

show abstract

Section: Lcm-seq Of Shoot Tissues In the Bolting-sensitive Line S39 Dmentioning

confidence: 99%

Section: Functional Characterization Of Lssoc1mentioning

confidence: 99%

See 1 more Smart Citation

LCM‐seq reveals the crucial role of LsSOC1 in heat‐promoted bolting of lettuce (Lactuca sativa L.)

Chen

Zhao

et al. 2018

The Plant Journal

Self Cite

View full text Add to dashboard Cite

show abstract

“…In 2016, the global production of lettuce and chicory reached 691,102 tons (http://www.fao.org/faostat/en/#data/QC/visualize). Because of global warming, lettuce production is often adversely affected by heat stress (Chen et al, ; Han et al, ). Lettuce is a heat‐induced bolting species that when exposed to heat stress conditions becomes bitter and begins to bolt and flower as well as transition from vegetative to reproductive growth.…”

Section: Introductionmentioning

confidence: 99%

Genome‐wide analysis of MADS‐box family genes during flower development in lettuce

Ning

Han

Chen

et al. 2019

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Lettuce (Lactuca sativa L.) is an important leafy vegetable consumed worldwide. Heat‐induced bolting and flowering greatly limit lettuce production during the summer. Additionally, MADS‐box transcription factors are important for various aspects of plant development and architecture (e.g., flowering and floral patterning). However, there has been no comprehensive study of lettuce MADS‐box family genes. In this study, we identified 82 MADS‐box family genes in lettuce, including 23 type I genes and 59 type II genes. Transcriptome profiling revealed that LsMADS gene expression patterns differ among the various floral stages and organs. Moreover, heat‐responsive cis‐elements were detected in the promoter regions of many LsMADS genes. An in situ hybridization assay of 10 homologs of flower‐patterning genes and a yeast two‐hybrid assay of the encoded proteins revealed that the ABC model is conserved in lettuce. Specifically, the APETALA1 (AP1) homolog in lettuce, LsMADS55, is responsive to heat and is specifically expressed in the inflorescence meristem and pappus bristles. The overexpression of LsMADS55 results in early flowering in Arabidopsis thaliana. Furthermore, we observed that the heat shock factor LsHSFB2A‐1 can bind to the LsMADS55 promoter in lettuce. Therefore, a model was proposed for the LsMADS‐regulated floral organ specification and heat‐induced flowering in lettuce.

show abstract

“…In some crops like Zea mays, Oryza sativa or Citrus sinensis, an early flowering phenotype has been selected either during domestication or by conventional breeding (Fujino et al, 2013;Hefny, 2011;Hung et al, 2012;Velazquez et al, 2016). In other cases like lettuce (Lactuca Sativa) or alfalfa itself, research has been performed to develop strategies that favour a late transition to reproductive stages, since the process of flowering, as mentioned earlier, has a negative impact on biomass quality (Aung et al, 2015;Chen et al, 2017;Gao et al, 2016;Jung et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Improvement of alfalfa forage quality and management through the down‐regulation of MsFTa1

Lorenzo

García‐Gagliardi

Antonietti

et al. 2019

Plant Biotechnology Journal

View full text Add to dashboard Cite

Summary Alfalfa (Medicago sativa L.) is one of the most important forage crops worldwide. As a perennial, alfalfa is cut several times each year. Farmers face a dilemma: if cut earlier, forage nutritive value is much higher but regrowth is affected and the longevity of the stand is severely compromised. On the other hand, if alfalfa is cut later at full flower, stands persist longer and more biomass may be harvested, but the nutritive value diminishes. Alfalfa is a strict long‐day plant. We reasoned that by manipulating the response to photoperiod, we could delay flowering to improve forage quality and widen each harvesting window, facilitating management. With this aim, we functionally characterized the FLOWERING LOCUS T family of genes, represented by five members: MsFTa1, MsFTa2, MsFTb1, MsFTb2 and MsFTc. The expression of MsFTa1 correlated with photoperiodic flowering and its down‐regulation led to severe delayed flowering. Altogether, with late flowering, low expression of MsFTa1 led to changes in plant architecture resulting in increased leaf to stem biomass ratios and forage digestibility. By manipulating photoperiodic flowering, we were able to improve the quality of alfalfa forage and management, which may allow farmers to cut alfalfa of high nutritive value without compromising stand persistence.

show abstract

Inflorescence Development and the Role of LsFT in Regulating Bolting in Lettuce (Lactuca sativa L.)

Cited by 51 publications

References 49 publications

LCM‐seq reveals the crucial role of LsSOC1 in heat‐promoted bolting of lettuce (Lactuca sativa L.)

LCM‐seq reveals the crucial role of LsSOC1 in heat‐promoted bolting of lettuce (Lactuca sativa L.)

Genome‐wide analysis of MADS‐box family genes during flower development in lettuce

Improvement of alfalfa forage quality and management through the down‐regulation of MsFTa1

Contact Info

Product

Resources

About