Inflammatory Response in the Early Stages of Wound Healing After Excimer Laser Keratectomy

Abstract

To evaluate the inflammatory response and its potential role in the early stages of corneal wound healing after excimer laser keratectomy.Materials and Methods: Lewis rats underwent excimer keratectomy using a 193-nm excimer laser. The central corneas were ablated in 3 depths: group A, epithelium; group B, superficial stroma; or group C, deep stroma. Eyes were harvested 1, 12, 24, and 36 hours, and 1 week after the rats were killed. Immunohistochemistry was used to test frozen sections with monoclonal antibodies of various inflammatory cellular markers. Results:Reepithelialization was observed at 12 hours in group A, and at 24 hours in groups B and C. Regenerated epithelium covered the denuded corneal surface in groups B and C after 1 week. The expression of major histocompatibility complex II antigen was detected in infiltrating cells, corneal epithelial cells, and endothelial cells 1 hour after surgery. Only a few macrophages and Langerhans cells were in the limbus at baseline. Macrophages migrated from the limbus to the corneal ablation zone and increased 2-fold after 36 hours in all 3 groups compared with baseline. Occasional lymphocytic infiltration was identified after 25 to 36 hours. Conclusion:Macrophages play an active role in the wound healing after laser keratectomy and may contribute to transient corneal haze.

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

“…This hypothesis is supported by other authors who observed that venom PLA 2 increases cytokines, such as IL-1, IL-6 and TNF-α (33). In our model, the number of mononuclear cells increased after LLLT, which agrees with the literature and shows that laser irradiation stimulates macrophages and lymphocytes (27,60).…”

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

“…This hypothesis is supported by other authors who observed that venom PLA 2 increases cytokines, such as IL-1, IL-6 and TNF-α (33). In our model, the number of mononuclear cells increased after LLLT, which agrees with the literature and shows that laser irradiation stimulates macrophages and lymphocytes (27,60).…”

The lectin KM+ induces corneal epithelial wound healing in rabbits

“…In this study, we observed a neutrophil influx into the corneal stroma 12 h after abrasion, a phenomenon that was enhanced in the KM+‐treated corneas. Moreover, our finding may indicate that the neutrophil accumulation in the wounded area is more relevant at an early phase of corneal repair, as previously demonstrated by other authors (O’Brien et al. 1998; Wilson et al.…”

Hepatocyte Growth Factor Suppresses Inflammation and Promotes Epithelium Repair in Corneal Injury

“…It is a well-known phenomenon that corneal injury leads to tissue infiltration of immune cells and release of pro-inflammatory cyto-kines. 28,29 Our in vivo results provide novel data that topical administration of HGF after corneal injury significantly reduces the infiltration of CD45 + inflammatory cells in the cornea. The massive infiltration of CD45 + inflammatory cells appears as a pseudoepithelial cell layer in the anterior stroma in control injured corneas.…”

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

“…This hypothesis is supported by other authors who observed that venom PLA 2 increases cytokines, such as IL-1, IL-6 and TNF-α (33). In our model, the number of mononuclear cells increased after LLLT, which agrees with the literature and shows that laser irradiation stimulates macrophages and lymphocytes (27,60).…”

The lectin KM+ induces corneal epithelial wound healing in rabbits

“…In this study, we observed a neutrophil influx into the corneal stroma 12 h after abrasion, a phenomenon that was enhanced in the KM+‐treated corneas. Moreover, our finding may indicate that the neutrophil accumulation in the wounded area is more relevant at an early phase of corneal repair, as previously demonstrated by other authors (O’Brien et al. 1998; Wilson et al.…”

Hepatocyte Growth Factor Suppresses Inflammation and Promotes Epithelium Repair in Corneal Injury

“…It is a well-known phenomenon that corneal injury leads to tissue infiltration of immune cells and release of pro-inflammatory cyto-kines. 28,29 Our in vivo results provide novel data that topical administration of HGF after corneal injury significantly reduces the infiltration of CD45 + inflammatory cells in the cornea. The massive infiltration of CD45 + inflammatory cells appears as a pseudoepithelial cell layer in the anterior stroma in control injured corneas.…”

Low-level laser therapy decreases local effects induced by myotoxins isolated from Bothrops jararacussu snake venom

“…This hypothesis is supported by other authors who observed that venom PLA 2 increases cytokines, such as IL-1, IL-6 and TNF-α (33). In our model, the number of mononuclear cells increased after LLLT, which agrees with the literature and shows that laser irradiation stimulates macrophages and lymphocytes (27,60).…”

The lectin KM+ induces corneal epithelial wound healing in rabbits

“…In this study, we observed a neutrophil influx into the corneal stroma 12 h after abrasion, a phenomenon that was enhanced in the KM+‐treated corneas. Moreover, our finding may indicate that the neutrophil accumulation in the wounded area is more relevant at an early phase of corneal repair, as previously demonstrated by other authors (O’Brien et al. 1998; Wilson et al.…”

Hepatocyte Growth Factor Suppresses Inflammation and Promotes Epithelium Repair in Corneal Injury

“…It is a well-known phenomenon that corneal injury leads to tissue infiltration of immune cells and release of pro-inflammatory cyto-kines. 28,29 Our in vivo results provide novel data that topical administration of HGF after corneal injury significantly reduces the infiltration of CD45 + inflammatory cells in the cornea. The massive infiltration of CD45 + inflammatory cells appears as a pseudoepithelial cell layer in the anterior stroma in control injured corneas.…”