Inflammatory response and cytokine levels induced by intralesional photodynamic therapy and 630-nm laser in a case series of basal cell carcinoma

Valladares, María Jesús Suárez; Calleja-Antolín, Sara; Morales, José María García Ruiz de; Prieto, Manuel Ángel Rodríguez; Vega-Gutiérrez, J

doi:10.1016/j.jaad.2018.02.060

Cited by 5 publications

(2 citation statements)

References 4 publications

(6 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thissen et al 17 demonstrated that an intracutaneous administration of ALA of concentration as low as 2% resulted in increased fluorescence levels and higher protoporphyrin IX concentrations compared to topical administration of 20% ALA. Cappugi et al reported the efficacy of this form of treatment in nodular BCC, and it has been described in the treatment of superficial BCC, squamous cell carcinoma and the treatment of parasitic diseases. [18][19][20][21] Suárez-Valladares et al 22 reported that in all BCC cases who underwent intralesional PDT, the levels of IL-1β, IL-8 and IL-6 increased after treatment indicating a distinct pattern of inflammatory response. The main limitation of our study was the small sample size of patients.…”

Section: Discussionmentioning

confidence: 99%

Intralesional photodynamic therapy induces apoptosis in basal cell carcinoma and Bowen's disease through caspase 3 and granzyme B

Evangelou

Koumaki

Fragiadaki

et al. 2023

Acad Dermatol Venereol

View full text Add to dashboard Cite

Basal cell carcinoma (BCC) is the most common neoplasm among white Caucasians. It can occur on both sunprotected and exposed sites reflecting the influence of acute and cumulative UVR exposure. 1 Bowen's disease is a form of intraepidermal squamous cell carcinoma, which usually occurs on the lower legs and predominantly in women in the seventh decade of life. Lesions may be solitary or multiple with different variants according to site or presentation including a palmar, genital, perianal and pigmented form. 2 Topical photodynamic therapy (PDT) is an effective, non-invasive treatment for non-melanoma skin cancer and certain non-malignant conditions. Topical PDT benefits from a relatively simple procedure that results in excellent cosmesis and cure rates comparable to other treatment modalities. 3 PDT is a strong inducer of apoptosis and/or necrosis through the generation of reactive oxygen species (ROS). 4 Apoptosis is a highly regulated biochemical

show abstract

Section: Discussionmentioning

confidence: 99%

Intralesional photodynamic therapy induces apoptosis in basal cell carcinoma and Bowen's disease through caspase 3 and granzyme B

Evangelou

Koumaki

Fragiadaki

et al. 2023

Acad Dermatol Venereol

View full text Add to dashboard Cite

show abstract

“…Once the inflammatory process is triggered in vitro, inflammatory mediators initiate the production of cytokines, which play key roles in the inflammatory response [51]. Previous studies have analyzed the action of PDT as a therapeutic method through the production of cytokines, observing an increase in pro-inflammatory cytokines (IL-1β, IL-8, IL-6 and MCP1), highlighting that the action of PDT has three phases: generation of ROS; expression of local endothelial damage and the mechanisms of the disease, so patients who responded to PDT had their levels of proinflammatory cytokines increased, as a response to the inflammatory process [52][53][54]. Also, in comparison with PDT and LED therapy, PDT proved to be more effective in reducing cytokine expression 24 hours after irradiation, when compared to the action of LED alone (therapeutic LED effect) [55,56].…”

Section: B Amentioning

confidence: 99%

Evaluation of Photodynamic Therapy as a New Therapeutic Approach Using Quinizarin-Loaded Nanocapsules: In Vitro Cytotoxicity, Permeation in 3D Bioprinted Skin Equivalent and In Vitro Cytokines Modulation

do Amaral,

Amantino,

Atanasov

et al. 2024

Preprint

View full text Add to dashboard Cite

Skin inflammation associated with chronic diseases has the direct action of keratinocytes in its immunopathogenesis, leading to a series of immune responses. However, there are still no highly targeted treatments, and the development of more specific therapies is highly desirable. In the present work, nanocapsules containing quinizarin (QZ/NC) were developed, evaluating their act in a model of the keratinocytes inflammatory process in vitro with the action of Photodynamic Therapy, analyzing the permeation in a 3D skin model. Physicochemical, stability, cytotoxicity and permeation analyses of the nanomaterials were carried out. The nanocapsules showed desirable physicochemical characteristics and were stable during the analysis period, without spectroscopic modifications. The cell viability test carried out showed no cytotoxicity at the lowest QZ/NC concentrations. The permeation and cellular uptake studies showed the permeation of QZ/NC in 3D skin models and the intracellular incorporation and internalization of the drug, enhancing its action in the drug delivery technique. Promising results were presented using the model developed to induce the inflammatory process in vitro and applying the synthesized nanomaterial in conjunction with PDT, where a decrease in the levels of cytokines analyzed was observed, representing a possible new therapeutic approach for inflammatory skin diseases.

show abstract

Photodynamic Therapy for the Treatment of Complex Anal Fistula

et al. 2019

View full text Add to dashboard Cite

Background and ObjectivesTo validate and analyze the results of intralesional photodynamic therapy in the treatment of complex anal fistula.Study Design/Materials and MethodsThis prospective multicentric observational study enrolled patients treated for complex anal fistula who underwent intralesional photodynamic therapy (i‐PDT). The included patients were treated from January 2016 to December 2018 with a minimum follow‐up of 1 year to evaluate recurrence, continence and postoperative morbidity. Intralesional 5‐aminolevulinic acid (ALA) gel (2%) was injected directly into the fistula. The internal and external orifices were closed. After an incubation period of 2 hours, the fistula was irradiated using an optical fiber connected to a red laser (Multidiode 630 PDT) operating at 1 W/cm for 3 minutes (180 J).ResultsIn total, 49 patients were included (61.2% male). The mean age was 48 years, and the mean duration of fistula was 13 months. Of the fistulas included, 75.5% were medium transphincteric, and 24.5% were high transphincteric. The median fistula length was 4 ± 1,14 cm (range: 3–5). A total of 41 patients (83.7%) had a previous history of fistula surgery. Preoperatively, some degree of anal incontinence was found in 5 patients (10.2%). No center reported any other procedure‐related complications intraoperatively. Phototoxicity was found in one patient. In the first 48 hours after the procedure, fever was reported in 2 patients (4%). At the end of follow‐up, total healing was observed in 32/49 patients (65.3%). No patient reported new incontinence postoperatively.Conclusioni‐PDT could be considered a good choice in patients with complex anal fistulas to avoid surgery and its complications. Lasers Surg. Med. © 2019 Wiley Periodicals, Inc.

show abstract

Inflammatory response and cytokine levels induced by intralesional photodynamic therapy and 630-nm laser in a case series of basal cell carcinoma

Abstract: Armstrong AW. Who do you prefer? A study of public preferences for health care provider type in performing cutaneous surgery and cosmetic procedures in the United States.

Cited by 5 publications

References 4 publications

Intralesional photodynamic therapy induces apoptosis in basal cell carcinoma and Bowen's disease through caspase 3 and granzyme B

Intralesional photodynamic therapy induces apoptosis in basal cell carcinoma and Bowen's disease through caspase 3 and granzyme B

Evaluation of Photodynamic Therapy as a New Therapeutic Approach Using Quinizarin-Loaded Nanocapsules: In Vitro Cytotoxicity, Permeation in 3D Bioprinted Skin Equivalent and In Vitro Cytokines Modulation

Photodynamic Therapy for the Treatment of Complex Anal Fistula

Contact Info

Product

Resources

About