Infectious Agents in Infant Diarrhea: I. A Hemagglutination-Inhibition Procedure for Detection of Bacterial Fractions in Infant Sera

Young, Viola Mae; Sochard, Minnie R.; Gillem, Harriette C.

doi:10.3181/00379727-105-26201

Cited by 10 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The 0127 lipopolysaccharide was a phenol-water preparation (List Biologicals, Campbell, Calif) [36]. The 0114 antigen was obtained by heat-alkaline treatment [37].…”

Section: Methodsmentioning

confidence: 99%

The Diarrheal Response of Humans to Some Classic Serotypes of Enteropathogenic Escherichia coli is Dependent on a Plasmid Encoding an Enteroadhesiveness Factor

Levine

Nataro

Karch

et al. 1985

Journal of Infectious Diseases

439

299

View full text Add to dashboard Cite

Isolates of the most common O serogroups of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea (designated class I) adhere to Hep-2 cells; the genes for this adhesin, termed EPEC adherence factor (EAF), are located on plasmids 50-70 MDa in size. Volunteers ingested 10(10) organisms of an O127:H6 Hep-2-adhesive class I strain (E2348/69) or its plasmid-minus, nonadhesive derivative. Diarrhea occurred in nine of 10 volunteers who ingested the parent strain (mean, 1,178 ml) but in only two of nine who took the plasmid-minus variant (mean, 433 ml; P less than .006). All volunteers ill from strain E2348/69 mounted serum IgA and IgG responses to a 94-kDa plasmid-associated outer membrane protein of E2348/69; this protein was found in other class I EPEC but not in enterotoxigenic or meningitic strains. The 50-70-MDa EAF plasmid seems necessary for full expression of pathogenicity in EPEC that exhibit Hep-2 adhesiveness. EPEC isolates of certain other, less common, O serogroups (O44, O86, and O114) are rarely Hep-2 adhesive. These EPEC, designated class II, possess distinct 50-70 MDa plasmids lacking EAF genes. Diarrhea was caused by 10(8) or 10(10) organisms of an O114:H2 class II EPEC strain (mean, 1,156 ml) in six of 11 volunteers. This result confirmed that class II EPEC are pathogenic by a mechanism not involving Hep-2 adhesiveness.

show abstract

“…The 0127 lipopolysaccharide was a phenol-water preparation (List Biologicals, Campbell, Calif) [36]. The 0114 antigen was obtained by heat-alkaline treatment [37].…”

Section: Methodsmentioning

confidence: 99%

The Diarrheal Response of Humans to Some Classic Serotypes of Enteropathogenic Escherichia coli is Dependent on a Plasmid Encoding an Enteroadhesiveness Factor

Levine

Nataro

Karch

et al. 1985

Journal of Infectious Diseases

439

299

View full text Add to dashboard Cite

show abstract

“…Sera were collected prechallenge and 10 and 21 to 28 days postchallenge. Alkaline extract lipopolysaccharide 0 antigen from each challenge strain was prepared by the method of Young et al (50); 0.1 ml of antigen was used to coat 1.0 ml of a 10% solution of glutaraldehyde-treated sheep erythrocytes (2). After heat inactivation (560C, 30 min) and absorption with unsensitized sheep erythrocytes, antisomatic antibody was measured by a microtiter passive hemagglutination technique (25).…”

mentioning

confidence: 99%

Immunity to Enterotoxigenic Escherichia coli

et al. 1979

View full text Add to dashboard Cite

Enterotoxigenic Escherichia coli strains represent the most frequent etiological agent of travelers diarrhea. Challenge studies with several of these strains were undertaken in volunteers to evaluate the mechanisms of disease-induced immunity. Seventeen students and other community volunteers were given 106 or 108 organisms of E. coli B7A (0148:H28), which produces heat-labile and heat-stable enterotoxins. Ten individuals developed diarrheal illness closely resembling natural travelers diarrhea; of these ten, rises in titer of serum antitoxin and anti-O antibody occurred in eight (80%). Eight of the volunteers who developed diarrhea in the first test agreed to undergo rechallenge 9 weeks later with 108 B7A organisms. Only one of these eight "veterans" developed diarrhea versus seven of twelve controls given the same challenge (P = 0.05). Despite clinical protection, all "veterans" excreted B7A after rechallenge. Four controls who developed diarrhea during the homologous B7A rechallenge test were rechallenged 9 weeks later with 109 organisms of E. coli strain E2528-C1 (025:H-), which produces only

show abstract

“…The method for endotoxin sensitization of RBC, without prior treatment of antigen, has been documented (5). A variation of this technique, i.e., heating the endotoxin at 100°C in a water bath for 60 min, was also done (18), as was a variation involving NaOH treatment of sensitizing antigen (26).…”

Section: Methodsmentioning

confidence: 99%

Analysis of Vibrio parahaemolyticus soluble antigens by employing passive hemagglutination

Sochard¹,

Colwell²

1979

J Clin Microbiol

View full text Add to dashboard Cite

The passive hemagglutination assay was explored as a sensitive test of immunological reactivity of endotoxin and other antigens prepared from selected strains of Vibrio parahaemolyticus. The tannic acid procedure for passive hemagglutination, commonly used with protein antigens, was the only procedure yielding good results with V. parahaemolyticus protein extracts, endotoxins, and related preparations. These results were probably due to the presence of large amounts of protein in the V. parahaemolyticus endotoxins as determined by earlier work referenced in the text. Glucose and galactose as possible antigenic determinants in the endotoxin of a Vibrio strain were tested by inhibition tests. Cross-reactions were observed between endotoxin preparations, but were low in hemagglutination, suggesting reactions of common generic antigens. The ability of V. parahaemolyticus endotoxins to stimulate production of antibodies was determined.

show abstract

Infectious Agents in Infant Diarrhea: I. A Hemagglutination-Inhibition Procedure for Detection of Bacterial Fractions in Infant Sera

Cited by 10 publications

References 0 publications

The Diarrheal Response of Humans to Some Classic Serotypes of Enteropathogenic Escherichia coli is Dependent on a Plasmid Encoding an Enteroadhesiveness Factor

The Diarrheal Response of Humans to Some Classic Serotypes of Enteropathogenic Escherichia coli is Dependent on a Plasmid Encoding an Enteroadhesiveness Factor

Immunity to Enterotoxigenic Escherichia coli

Analysis of Vibrio parahaemolyticus soluble antigens by employing passive hemagglutination

Contact Info

Product

Resources

About