Infection trials in pigs with a human isolate of<i>Brucella</i>(isolate 02/611 ‘marine mammal type’)

Bingham, John; Taylor, Tamara; Swingler, J.; Meehan, Greer; Middleton, DJ; Mackereth, GF; O’Keefe, John; Daniels, P.W.

doi:10.1080/00480169.2008.36798

Cited by 6 publications

(7 citation statements)

References 21 publications

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“…Moreover, within the zoonotic brucellae there are some species that are more virulent than others (e.g., B. melitensis > B. suis biovars 1, 3, and 4 ≥ B. abortus > B. canis ; Spink, 1956; Bosseray et al, 1982; Ruiz-Castañeda, 1986; Caron et al, 1994). In contrast, Brucella ceti and Brucella pinnipedialis preferentially infecting free living cetaceans and pinnipeds, respectively, have seldom been found in other animal groups and their zoonotic potential and overall virulence for other animal species, including bovine and swine, seem low (Rhyan et al, 2001a; Perrett et al, 2004; Bingham et al, 2008; Guzmán-Verri et al, 2012). Likewise, Brucella species and strains (e.g., B. neotomae , B. microti , and B. suis biovar 5) having preference for wild land mammals are confined to their natural hosts and seldom found in domestic animals or humans (Moreno and Moriyón, 2006; Al Dahouk et al, 2012).…”

Section: Artificial Selection Of Brucellamentioning

confidence: 98%

Retrospective and prospective perspectives on zoonotic brucellosis

2014

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Members of the genus Brucella are pathogenic bacteria exceedingly well adapted to their hosts. The bacterium is transmitted by direct contact within the same host species or accidentally to secondary hosts, such as humans. Human brucellosis is strongly linked to the management of domesticated animals and ingestion of their products. Since the domestication of ungulates and dogs in the Fertile Crescent and Asia in 12000 and 33000 ya, respectively, a steady supply of well adapted emergent Brucella pathogens causing zoonotic disease has been provided. Likewise, anthropogenic modification of wild life may have also impacted host susceptibility and Brucella selection. Domestication and human influence on wild life animals are not neutral phenomena. Consequently, Brucella organisms have followed their hosts’ fate and have been selected under conditions that favor high transmission rate. The “arm race” between Brucella and their preferred hosts has been driven by genetic adaptation of the bacterium confronted with the evolving immune defenses of the host. Management conditions, such as clustering, selection, culling, and vaccination of Brucella preferred hosts have profound influences in the outcome of brucellosis and in the selection of Brucella organisms. Countries that have controlled brucellosis systematically used reliable smooth live vaccines, consistent immunization protocols, adequate diagnostic tests, broad vaccination coverage and sustained removal of the infected animals. To ignore and misuse tools and strategies already available for the control of brucellosis may promote the emergence of new Brucella variants. The unrestricted use of low-efficacy vaccines may promote a “false sense of security” and works towards selection of Brucella with higher virulence and transmission potential.

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Section: Artificial Selection Of Brucellamentioning

confidence: 98%

Retrospective and prospective perspectives on zoonotic brucellosis

2014

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“…Ten piglets were challenged with marine mammal Brucella spp. [53], originally isolated from a human [54]. No pathological changes were detected, transient and low antibody titres were recorded from three of the piglets, and bacteria were isolated from one lymph node of three other piglets.…”

Section: Infection With B Ceti and B Pinnipedialismentioning

confidence: 99%

“…No pathological changes were detected, transient and low antibody titres were recorded from three of the piglets, and bacteria were isolated from one lymph node of three other piglets. The marine mammal isolate was unable to establish a sustained infection in the piglets [53]. Experimental infection in pregnant sheep with marine mammal Brucella isolates from seal, porpoise and dolphin resulted in limited seroconversion in the sheep.…”

Section: Infection With B Ceti and B Pinnipedialismentioning

confidence: 99%

A review of Brucella infection in marine mammals, with special emphasis on Brucella pinnipedialis in the hooded seal (Cystophora cristata)

Nymo

Tryland

Godfroid

2011

Vet Res

118

131

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Brucella spp. were isolated from marine mammals for the first time in 1994. Two novel species were later included in the genus; Brucella ceti and Brucella pinnipedialis, with cetaceans and seals as their preferred hosts, respectively. Brucella spp. have since been isolated from a variety of marine mammals. Pathological changes, including lesions of the reproductive organs and associated abortions, have only been registered in cetaceans. The zoonotic potential differs among the marine mammal Brucella strains. Many techniques, both classical typing and molecular microbiology, have been utilised for characterisation of the marine mammal Brucella spp. and the change from the band-based approaches to the sequence-based approaches has greatly increased our knowledge about these strains. Several clusters have been identified within the B. ceti and B. pinnipedialis species, and multiple studies have shown that the hooded seal isolates differ from other pinniped isolates. We describe how different molecular methods have contributed to species identification and differentiation of B. ceti and B. pinnipedialis, with special emphasis on the hooded seal isolates. We further discuss the potential role of B. pinnipedialis for the declining Northwest Atlantic hooded seal population.

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“…Experimental infection in piglets ( Sus scrofa domesticus ) with a marine mammal brucellae human isolate 02/611 [ 18 ], belonging to ST27 [ 13 ], yielded no pathology [ 21 ], while abortion has been induced in cattle ( Bos taurus ) after infection with a B . pinnipedialis Pacific harbour seal ( Phoca vitulina richardsi ) strain [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Marine Mammal Brucella Reference Strains Are Attenuated in a BALB/c Mouse Model

et al. 2016

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Brucellosis is a zoonosis of worldwide distribution with numerous animal host species. Since the novel isolation of Brucella spp. from marine mammals in 1994 the bacteria have been isolated from various marine mammal hosts. The marine mammal reference strains Brucella pinnipedialis 12890 (harbour seal, Phoca vitulina) and Brucella ceti 12891 (harbour porpoise, Phocoena phocoena) were included in genus Brucella in 2007, however, their pathogenicity in the mouse model is pending. Herein this is evaluated in BALB/c mice with Brucella suis 1330 as a control. Both marine mammal strains were attenuated, however, B. ceti was present at higher levels than B. pinnipedialis in blood, spleen and liver throughout the infection, in addition B. suis and B. ceti were isolated from brains and faeces at times with high levels of bacteraemia. In B. suis-infected mice serum cytokines peaked at day 7. In B. pinnipedialis-infected mice, levels were similar, but peaked predominantly at day 3 and an earlier peak in spleen weight likewise implied an earlier response. The inflammatory response induced pathology in the spleen and liver. In B. ceti-infected mice, most serum cytokine levels were comparable to those in uninfected mice, consistent with a limited inflammatory response, which also was indicated by restricted spleen and liver pathology. Specific immune responses against all three strains were detected in vitro after stimulation of splenocytes from infected mice with the homologous heat-killed brucellae. Antibody responses in vivo were also induced by the three brucellae. The immunological pattern of B. ceti in combination with persistence in organs and limited pathology has heretofore not been described for other brucellae. These two marine mammal wildtype strains show an attenuated pattern in BALB/c mice only previously described for Brucella neotomea.

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Infection trials in pigs with a human isolate ofBrucella(isolate 02/611 ‘marine mammal type’)

Abstract: Brucella 02/611 does not seem to replicate readily in pigs. It is unlikely that pigs were the original maintenance hosts for Brucella 02/611.

Cited by 6 publications

References 21 publications

Retrospective and prospective perspectives on zoonotic brucellosis

Retrospective and prospective perspectives on zoonotic brucellosis

A review of Brucella infection in marine mammals, with special emphasis on Brucella pinnipedialis in the hooded seal (Cystophora cristata)

Marine Mammal Brucella Reference Strains Are Attenuated in a BALB/c Mouse Model

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