Infection by Schistosoma mansoni sambon 1907 in the first four months of life of Biomphalaria straminea (Dunker, 1848) in Brazil

Fernandez, Mônica Ammon; Pieri, Otávio Sarmento

doi:10.1590/s0074-02762001000900029

Cited by 11 publications

(7 citation statements)

References 17 publications

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“…However, the exposed positive snails (n = 13) presented an 86.6% survival rate. This difference has been attributed either to the parasite strain or to the susceptibility profile, age and size of the mollusc population, in addition to the time elapsed since exposure (Sorensen and Minchella, 2001;Fernandez and Pieri, 2001). These results might be associated with the finding that the development of the larvae is slower in infected snails that do not release cercariae, leading to gradual consumption of the energy reserves.…”

Section: Resultsmentioning

confidence: 99%

Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Faro

Perazzini

Corrêa

et al. 2013

Experimental Parasitology

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Parasitic castration in the snail-trematode relationship can be understood as any change in the reproductive function of the snail that is due to interference by the developing larvae inside the snail that leads to the reduction or complete disruption of egg-laying activity. This study was designed to observe the parasitic castration of Biomphalaria glabrata infected with Schistosoma mansoni during both the pre-patent and patent periods. The effect of infection on snail fecundity and fertility, growth rate and survival was studied during the 62 days following miracidia exposure. An integrated approach was employed that used biochemical and histological tools over the same period. To study the effect of infection on reproduction, we individually exposed 30 snails to 5 miracidia each and tracked their fertility and fecundity. For our histopathological studies, 50 snails were exposed to 20 miracidia each, and for our histochemical studies, 50 snails were exposed to 5 miracidia each. An equal number of uninfected snails were used as a control for each group. The B. glabrata exposed to the BH strain of S. mansoni showed 50% positivity for cercarial shedding. Both the experimental and control groups showed 100% survival. The pre-patent period lasted until 39 days after exposure to miracidia. Exposed snails that showed cercarial shedding exhibited higher growth rates than either exposed snails that did not demonstrate cercarial shedding or uninfected controls. Exposed snails without cercarial shedding and uninfected controls showed no differences in the reproductive parameters evaluated during the patent period; snails experiencing cercarial shedding showed a reduction in fecundity and fertility. These snails began to lay eggs only after the 50th day post miracidia exposure. The haemolymph glucose levels showed an oscillating pattern that decreased during periods of greater mobilisation of energy by the larvae and was accompanied by a depletion of glycogen in the cephalopodal mass and digestive gland. Histopathological examination at 55 days showed that the ovotestis was highly atrophied. There was almost complete disappearance of germ cells, and the supporting stroma formed a nearly empty net. At day 45, the infected digestive gland showed a high cylindrical epithelium with little preserved cytoplasm. The contents of the secretory granules of the albumen gland of infected animals stained with Alcian blue (AB), pH 1.0, indicating the presence of sulphated carbohydrates. Thus, parasitic castration in the B. glabrata-S. mansoni model may be regulated directly and indirectly by the developmental stage of the trematode and the biochemical and histopathological alterations during the patent period of infection.

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Section: Resultsmentioning

confidence: 99%

Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Faro

Perazzini

Corrêa

et al. 2013

Experimental Parasitology

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“…In the north-east of Brazil, the prevalence of S. mansoni infection in B. straminea ranges from 11 to 24% [38, 39], while artificial exposures of S. mansoni to B. straminea in the laboratory have shown low infectivity, with prevalence ranging from 1.2 to 1.6% depending on the strain of miracidia being tested [40]. Close to the border between Hong Kong and mainland China, in the city of Shenzhen, a recent study reported that the black-coloured B. straminea collected in the field were resistant to S. mansoni infection [41].…”

Section: Discussionmentioning

confidence: 99%

Distribution and current infection status of Biomphalaria straminea in Hong Kong

et al. 2017

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BackgroundSchistosomiasis, also generally known as snail fever, is a parasitic disease caused by trematode flatworms of the genus Schistosoma. In Hong Kong and mainland China, the freshwater snail Biomphalaria straminea has been introduced and has the potential to transmit intestinal schistosomiasis caused by S. mansoni, a parasite of man which has a wide distribution in Africa and parts of the New World, especially Brazil. The first identification of B. straminea in Hong Kong dates back to the 1970s, and its geographical distribution, phylogenetic relationships, and infection status have not been updated for more than 30 years. Thus, this study aims to reveal the distribution and current infection status of B. straminea in contemporary Hong Kong.MethodsSnails were collected from different parts of Hong Kong from July 2016 to January 2017. Both anatomical and molecular methods were applied to identify B. straminea. Cytochrome c oxidase subunit 1 (cox1), internal transcribed spacer 1 (ITS1), 5.8S rDNA, internal transcribed spacer 2 (ITS2), and 16S ribosomal DNA (rDNA) were sequenced from individual snails and analyzed. To detect the presence of S. mansoni, both biopsy and PCR analyses were carried out.ResultsUsing both anatomical and molecular analyses, this study demonstrated the existence of black- and red-coloured shell B. straminea in different districts in the New Territories in Hong Kong, including places close to the mainland China border. None of the B. straminea (n = 87) investigated were found to be infected with S. mansoni when tested by biopsy and PCR. The Hong Kong B. straminea are genetically indistinguishable, based on the chosen molecular markers (cox1, ITS1-5.8S-ITS2, and 16S rDNA), and are similar to those obtained in mainland China and South America.Conclusion Biomphalaria straminea is now well established in freshwater habitats in Hong Kong. No evidence of infection with S. mansoni has been found. Surveillance should be continued to monitor and better understand this schistosomiasis intermediate host in mainland China and Hong Kong.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-017-2285-3) contains supplementary material, which is available to authorized users.

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“…The snails were individually exposed to 5 Schistosoma mansoni miracidia from Camorim, State of Pernambuco (CM strain), Picos, Piauí (EC strain) and Padre Bernardo, Goiás (PB strain). The CM and EC strains were isolated as described by Fernandez and Pieri (2001). The PB strain was isolated on June 30, 1998 from feces of VP Silva, 21 years, born and grown up in Amorim, Padre Bernardo, GO, an urban focus of schistosomiasis since 1997 (Amaral & Alves 1997), ranging approximately 300 km from the lake of the UHSM.…”

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confidence: 99%

“…However, infection indices up 10% were obtained by some investigators: Paraense (1967) obtained 3 positive snails of 28 specimens from Fordlândia, Pará (index of 10.7%); Souza et al (1983) found 11 out of 100 specimens from Jequitibá, Minas Gerais (11%); and Fernandez and Pieri (2001) found 217 positive snails among 1,691 specimens from Camorim, Pernambuco (12.8%). The lengthening of the precercarial period is also in accordance with that described by Favre et al (1995) and Fernandez and Pieri (2001) for this species.…”

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confidence: 99%

Susceptibility of Biomphalaria straminea (Dunker, 1848) from Serra da Mesa Dam, Goiás, Brazil to infection with three strains of Schistosoma mansoni Sambon, 1907

Fernandez

Thiengo

2002

Mem. Inst. Oswaldo Cruz

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Infection by Schistosoma mansoni sambon 1907 in the first four months of life of Biomphalaria straminea (Dunker, 1848) in Brazil

Cited by 11 publications

References 17 publications

Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Biological, biochemical and histopathological features related to parasitic castration of Biomphalaria glabrata infected by Schistosoma mansoni

Distribution and current infection status of Biomphalaria straminea in Hong Kong

Susceptibility of Biomphalaria straminea (Dunker, 1848) from Serra da Mesa Dam, Goiás, Brazil to infection with three strains of Schistosoma mansoni Sambon, 1907

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