Inexpensive Procedure for Measurement of Ethanol: Application to Bioethanol Production Process

Miah, Roni; Siddiqa, Ayesha; Tuli, Jamsheda Ferdous; Barman, Noyon Kumar; Dey, Shuvra Kanti; Adnan, Nihad; Yamada, Mamoru; Talukder, Ali Azam

doi:10.4236/aim.2017.711059

Cited by 16 publications

(6 citation statements)

References 12 publications

(18 reference statements)

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“…Ethanol concentration was measured by the solvent extraction and dichromate oxidation method 24 . Isolates C-7, M-3 and Dfs-1 produced low ethanol concentration- less than 1.5% (v/v) from 10.0% starch at different growth temperatures (30, 37 and 42 °C) are shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

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Development of high temperature simultaneous saccharification and fermentation by thermosensitive Saccharomyces cerevisiae and Bacillus amyloliquefaciens

Miah

Siddiqa

Chakraborty

et al. 2022

Sci Rep

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Scarcity of energy and pollution are two major challenges that have become a threat to all living things worldwide. Bioethanol is a renewable, ecological-friendly clean energy that may be utilized to address these issues. This study aimed to develop simultaneous saccharification and fermentation (SSF) process through high temperature-substrate adaptation and co-cultivation of S. cerevisiae with other potential amylolytic strains. In this study, we adapted our previously screened thermosensitive Saccharomyces cerevisiae Dj-3 strain up-to 42 °C and also screened three potential thermotolerant amylolytic strains based on their starch utilization capability. We performed SSF fermentation at high temperature by adapted Dj-3 and amylolytic strains using 10.0% starch feedstock. Interestingly, we observed significant ethanol concentration [3.86% (v/v)] from high temperature simultaneous saccharification and fermentation (HSSF) of adapted Bacillus amyloliquefaciens (C-7) and Dj-3. We attribute the significant ethanol concentration from starch of this HSSF process to C-7’s high levels of glucoamylase activity (4.01 U/ml/min) after adaptation in starch (up-to 42 °C) as well as Dj-3's strong glucose fermentation capacity and also their ethanol stress tolerance capability. This study suggests the significant feasibility of our HSSF process.

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Section: Resultsmentioning

confidence: 99%

“…After centrifugation, the supernatant was collected and filtered using a syringe filter (0.22–0.45 μM). The ethanol concentration in the sample was estimated using previously developed solvent extraction and dichromate oxidation method 24 .…”

Section: Methodsmentioning

confidence: 99%

Development of high temperature simultaneous saccharification and fermentation by thermosensitive Saccharomyces cerevisiae and Bacillus amyloliquefaciens

Miah

Siddiqa

Chakraborty

et al. 2022

Sci Rep

Self Cite

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“…Solution were again vortexed until phases separates out. Then, lower phase was taken and diluted up to 10 times with standerd solutions and sample ( Miah et al, 2017 ). Optical densities of solution were measured at 595 nm (OD 595 ) in UV-spectrophotometer (SPECORD 200 plus, analytikjena Germany).…”

Section: Methodsmentioning

confidence: 99%

Deciphering role of technical bioprocess parameters for bioethanol production using microalgae

Bibi

Yasmin

Jamal

et al. 2021

Saudi Journal of Biological Sciences

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Microalgae biomass is considered an important feedstock for biofuels and other bioactive compounds due to its faster growth rate, high biomass production and high biomolecules accumulation over first and second-generation feedstock. This research aimed to maximize the specific growth rate of fresh water green microalgae Closteriopsis acicularis, a member of family Chlorellaceae under the effect of pH and phosphate concentration to attain enhanced biomass productivity. This study investigates the individual and cumulative effect of phosphate concentration and pH on specific growth characteristics of Closteriopsis acicularis in autotrophic mode of cultivation for bioethanol production. Central-Composite Design (CCD) strategy and Response Surface Methodology (RSM) was used for the optimization of microalga growth and ethanol production under laboratory conditions. The results showed that high specific growth rate and biomass productivity of 0.342 day −1 and 0.497 g L −1 day −1 respectively, were achieved at high concentration of phosphate (0.115 g L −1 ) and pH (9) at 21st day of cultivation. The elemental composition of optimized biomass has shown enhanced elemental accumulation of certain macro (C, O, P) and micronutrients (Na, Mg, Al, K, Ca and Fe) except for nitrogen and sulfur. The Fourier transform infrared spectroscopic analysis has revealed spectral peaks and high absorbance in spectral range of carbohydrates, lipids and proteins, in optimized biomass. The carbohydrates content of optimized biomass was observed as 58%, with 29.3 g L −1 of fermentable sugars after acid catalyzed saccharification. The bioethanol yield was estimated as 51 % g ethanol/g glucose with maximum of 14.9 g/L of bioethanol production. In conclusion, it can be inferred that high specific growth rate and biomass productivity can be achieved by varying levels of phosphate concentration and pH during cultivation of Closteriopsis acicularis for improved yield of microbial growth, biomass and bioethanol production.

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“…This paper concerns calculations involved with determination of ethanol contents, taken as interesting examples of classical, titrimetric analysis. Importance of classical determination of this analyte is revealed by the facts that: (1) a variety of analytical procedures were suggested for the ethanol determination [1][2][3][4][5][6][7][8][9][10][11]; (2) the international ethanol reference solution is prepared by the dichromate oxidation method [12].…”

Section: Introductory Remarksmentioning

confidence: 99%

Stoichiometric approach to redox back titrations in ethanol analyses

Michałowska-Kaczmarczyk¹,

Michałowski²

2019

Ann Adv Chem

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This article refers to calculations involved with determination of ethanol, analyzed according to redox back titration principle. A quantitative reasoning, based on logical sequence of statements, is presented for derivation of the formulas required to calculate the results of chemical analyses according to stoichiometric principles. The titrations are considered as two-step analytical procedures. This way, one can gain an insight into a classical redox titration and get a knowledge on the advantages of back titrations.

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Inexpensive Procedure for Measurement of Ethanol: Application to Bioethanol Production Process

Cited by 16 publications

References 12 publications

Development of high temperature simultaneous saccharification and fermentation by thermosensitive Saccharomyces cerevisiae and Bacillus amyloliquefaciens

Development of high temperature simultaneous saccharification and fermentation by thermosensitive Saccharomyces cerevisiae and Bacillus amyloliquefaciens

Deciphering role of technical bioprocess parameters for bioethanol production using microalgae

Stoichiometric approach to redox back titrations in ethanol analyses

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