Inductions of Ornithine Decarboxylase and Replicative DNA Synthesis but not DNA Single Strand Scission or Unscheduled DNA Synthesis in the Pyloric Mucosa of Rat Stomach by Catechol

Furihata, Chie; Hatta, Akiko; Matsushima, Taijiro

doi:10.1111/j.1349-7006.1989.tb02258.x

Cited by 14 publications

(7 citation statements)

References 19 publications

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“…3). With the 0.1 °~o dose, erosion was observed from 12 hr to day 3, but no remarkable changes were evident on day 7. The thickness of the prepyloric region of the glandular stomach epithelium initially increased at day 1 in the 1 and 0.5% groups and at day 2 in the 0.1 % group.…”

Section: Sequential Morphological Changesmentioning

confidence: 86%

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Sequential Morphological and Biological Changes in the Glandular Stomach Induced by Oral Administration of Catechol to Male F344 Rats

et al. 1999

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Histogenesis and mechanisms of catechol-induced rat glandular stomach carcinogenesis were investigated in male F344 rats. Groups of 5 or 6 rats were treated with dietary catechol at doses of 1, 0.5, 0.1, and 0.01% for 12 hr or for 1, 2, 3, or 7 days or at a dose of 0.8% for 1, 2, 4, 12, and 24 wk; rats were then euthanatized. The initial morphological changes were edema of the gastric wall, inflammatory-cell infiltration, erosion in the pyloric region close to the duodenum, and considerable increase in apoptosis at 12 hr; later, changes included augmented DNA synthesis and cell proliferation, as evaluated by bromodeoxyuridine labeling index and thickness of mucosa, respectively, on day 1. Downward hyperplasia due to excess regeneration appeared at edges of ulceration at week 2. This lesion disappeared, and then submucosal hyperplasia appeared in the course of adenoma development. Only slight expression of c -myc or c-fos was apparent after 30-min oral administration or 1-, 3-, and 6-hr oral administration of catechol. No increase in lipid peroxide levels was evident in gastric epithelium fed catechol for 1 wk.

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Section: Sequential Morphological Changesmentioning

confidence: 86%

“…Erosion/ulcer continued to be present for 7 days, but inflammatory cell infiltration gradually decreased with time. In the early stage, small, focal, and multiple superficial erosions were found throughout the pyloric region, but they became preferentially localized at the prepylorus on day 7 (Fig. 3).…”

Section: Sequential Morphological Changesmentioning

confidence: 99%

Sequential Morphological and Biological Changes in the Glandular Stomach Induced by Oral Administration of Catechol to Male F344 Rats

et al. 1999

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“…Kawanishi et al (1989) reported that Pyrocatechol did not induce DNA cleavage. Unscheduled DNA synthesis was induced by Pyrocatechol in three studies with MRC-Wistar rats (Mirvish et al, 1985), but one study with F344 rats was negative (Furihata et al, 1989). Pyrocatechol inhibited DNA synthesis by 65% in mouse lymphoma cells (PellackWalker et al, 1985) and by 52% in bone marrow cells (Lee et al, 1989) but had no effect upon a cell-free DNA synthetic system (Lee et al, 1989) or bladder epithelial cells from rats (Kurata et al, 1990).…”

Section: Mutagenicitymentioning

confidence: 96%

“…Negative results were obtained in studies with supercoiled <})X-174 DNA (Lewis et al, 1988b) an mouse lymphoma cells (Pellack-Walker and Blumer, 1986). Also, no increase in single-strand scission was observed in DNA of the gastric pyloric mucosa of rats administered Pyrocatechol by gastric intubation (Furihata et al, 1989). Kawanishi et al (1989) reported that Pyrocatechol did not induce DNA cleavage.…”

Section: Mutagenicitymentioning

confidence: 96%

Amended Final Report On the Safety Assessment of Pyrocatechol

Andersen¹

1997

Int J Toxicol

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Pyrocatechol is a phenol used primarily in hair dyes but has one reported use in skin care preparations. When this ingredient was first reviewed it was concluded that Pyrocatechol was safe for use in formulations designed for discontinuous, brief use followed by rinsing from the skin and hair at concentrations of up to 1.0%. This amendment to that report was prepared in order to incorporate the results from several studies, including those reporting immunologic, carcinogenic, and cocarcinogenic effects of Pyrocatechol. In vitro, Pyrocatechol has been shown to affect several immunologic and other properties of murine bone marrow cells, both alone and when combined with hydroquinone. Pyrocatechol, when produced as an hepatic metabolite of benzene, has been reported to concentrate in the bone marrow and to adversely affect hematopoietic precursor cells. These effects are somewhat species specific. In vivo, dermal exposure of mice to Pyrocatechol affects the number and distribution of Langerhans cells at the site of exposure but is not associated with a change in contact sensitivity. Oral exposure of pregnant rats to Pyrocatechol did result in an increase in teratogenic effects. The results of a micromass teratogen test in vitro were also positive. Pyrocatechol was tested in a number of bacterial and mammalian mutagenesis systems. Both negative and positive results were observed. Carcinogenicity studies were conducted in rats and mice. Pyrocatechol was provided in the feed. While adenomatous hyperplasia was noted in both species, adenocarcinomas were seen in male and female rats only. In cocarcinogenesis studies there is a similar pattern of hyperplasia and adenomas of the glandular stomach in the groups exposed to Pyrocatechol alone. When administered with several other carcinogens, Pyrocatechol had a cocarcinogenic effects. Pyrocatechol was not a tumor promoter in dermal studies. Based on these data and the significant potential for skin absorption of Pyrocatechol in leave-on preparations, it was concluded that Pyrocatechol is unsafe for use in leave-on cosmetic formulations. Pyrocatechol used in a rinseoff application such as hair dyes may not present a similar concern if Pyrocatechol is completely and rapidly oxidized. Because no data were available, it was concluded that there are insufficient data to support the safety of this ingredient. The data needed to complete the safety assessment include the extent and rate of oxidation when used in hair dyeing. If not rapidly and completely oxidized, then further chemical characterization of cosmetic grade Pyrocatechol is needed, along with cutaneous absorption data in aqueous and at RYERSON UNIV on June 15, 2015 ijt.sagepub.com Downloaded from

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“…The time-dependency of ODC activity induction in the pyloric mucosa by taurocholate, NaCl, catechol, K2S2O5, formaldehyde and glyoxal was transient and reached a maximum after 4 h by taurocholate (72), 6-9 h by NaCl (78) and catechol (63), and 16 h by glyoxal (68), formaldehyde (80) and K2S2O5 (81). ODC activity returned to the control level within 48 h after administration of these compounds (57).…”

Section: Attempts At Organ-speciˆc In Vivo Short-term Assays For the mentioning

confidence: 97%

Attempts at Organ-specific In Vivo Short-term Tests for Environmental Mutagens and Carcinogens in Rodent Liver and Stomach

Furihata

2013

Genes and Environment

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The primary motivation for conducting short-term tests for environmental mutagens and carcinogens has been to predict mutagens and/or carcinogens and to assess any associated risks. Organ-speciˆc in vivo short-term tests in rodents are valuable because chemical carcinogenesis is generally organ-speciˆc. I have attempted to develop various organ-speciˆc in vivo short-term tests mainly in rodent liver and stomach. Recently, our collaborative study group, Toxicogenomics/Japanese Environmental Mutagen Society･Mammalian Mutagenicity Study Group (JEMS･ MMS), attempted to use gene expression proˆling in in vivo short-term tests, conducted DNA microarrays to extract candidate marker genes, and later shifted to quantitative real-time PCR (qPCR) to proˆle the expression of selected genes. We successfully discriminated 8 genotoxic hepatocarcinogens from 4 non-genotoxic hepatocarcinogens by statistical analysis using principal component analysis (PCA) based on the gene expression proˆles for 12 genes (Aen, Bax, Btg2, Ccnf, Ccng1, Cdkn1a, Gdf15, Lrp1, Mbd1, Phlda3, Plk2, and Tubb2c) in mouse liver at 4 and 48 h following a single intraperitoneal administration of chemicals as determined by qPCR. More recently, we successfully performed a similar study in rat liver. Previously, my collaborators and I developed various organspeciˆc in vivo short-term test methods, including UDS (unscheduled DNA synthesis); RDS (replicative DNA synthesis) using a liquid scintillation counter in rat glandular stomach, forestomach, colon, and liver and hairless mouse epidermis; DNA single-strand scission (DSS); and ornithine decarboxylase assay (ODC) in rat glandular stomach on 62 compounds. Developing short-term tests that are helpful for the risk assessment of human mutagens and carcinogens would contribute to the development of ideal prediction methods.

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Inductions of Ornithine Decarboxylase and Replicative DNA Synthesis but not DNA Single Strand Scission or Unscheduled DNA Synthesis in the Pyloric Mucosa of Rat Stomach by Catechol

Cited by 14 publications

References 19 publications

Sequential Morphological and Biological Changes in the Glandular Stomach Induced by Oral Administration of Catechol to Male F344 Rats

Sequential Morphological and Biological Changes in the Glandular Stomach Induced by Oral Administration of Catechol to Male F344 Rats

Amended Final Report On the Safety Assessment of Pyrocatechol

Attempts at Organ-specific In Vivo Short-term Tests for Environmental Mutagens and Carcinogens in Rodent Liver and Stomach

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