Induction of tumor clones in D. melanogaster wts/+ heterozygotes with chemical carcinogens

Sidorov, Roman A.; Ugnivenko, E.G.; Khovanova, E M; Belitsky, Gennady A.

doi:10.1016/s1383-5718(01)00277-7

Cited by 28 publications

(30 citation statements)

References 14 publications

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“…The distribution of these tumors was restricted to the wings (9 tumors), body (5 tumors) and legs (3 tumors). This rate of spontaneous frequency, with a distribution of tumors primarily on the wings and the body of the fly, was also reported by Sidorov et al (2001). They indicated that the tumor frequencies on the wing and on the notum are the highest among all the organs because they have the largest number of cells and the longest period of cell proliferation.…”

Section: Resultssupporting

confidence: 67%

“…Considering that the appearance of tumors is present only in the homozygosis phase due to genetic loss, wts is thought to be a tumor suppressor gene (Justice et al, 1995). Sidorov et al (2001) induced epithelial tumor clones in Drosophila using a lineage heterozygotic for the tumor suppressor gene wts. They studied 10 compounds of five chemical groups: polycyclic aromatic hydrocarbonates (PAH), aromatic amines, derivatives of heterocyclic aromatic N-nitrosamines, and platinum complex materials, to see how efficient they were at generating tumors.…”

Section: Introductionmentioning

confidence: 99%

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Lapachol as an epithelial tumor inhibitor agent in Drosophila melanogaster heterozygote for tumor suppressor gene wts

Costa¹,

Oliveira²,

Nepomuceno³

2011

Genet. Mol. Res.

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ABSTRACT. The search for new and effective antitumor agents with fewer cytotoxic side effects on normal tissue has increasingly become important. Lapachol, a natural organic compound isolated from the lapacho tree (Tabebuia avellandedae), is chemically identified as belonging to the naphthoquinone group and is known for its antiinflammatory, analgesic and antibiotic properties, although there are questions about its effectiveness for treating neoplasic cells. We evaluated the antitumoral effects of lapachol by testing for clones of epithelial tumors in Drosophila melanogaster. Seventy-two-hour old larvae bred from wts/TM3, Sb 1 females and mwh/mwh males, were treated with different concentrations of lapachol (20, 40 and 60 µg/mL). Lapachol alone did not significantly increase the number of epithelial tumors. However, lapachol did significantly reduce the number of tumors provoked by doxorubicin.

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Section: Resultssupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

Lapachol as an epithelial tumor inhibitor agent in Drosophila melanogaster heterozygote for tumor suppressor gene wts

Costa¹,

Oliveira²,

Nepomuceno³

2011

Genet. Mol. Res.

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“…There are higher probabilities of heterozygosis loss of the wts marker in locations with a large number of cells. The results obtained in the present investigation, for tumor frequency in the D. melanogaster appendices are in agreement with data in the literature, which shows a greater tendency to heterozygosis loss of the wts gene in appendices with a greater number of cells (SIDOROV et al, 2001;NEPOMUCENO, 2009;COSTA et al, 2011;FURTADO;NEPOMUCENO, 2012).…”

Section: Resultssupporting

confidence: 92%

“…The Epithelial Tumor Detection Test in D. melanogaster is a test used to diagnose tumor in flies, caused by xenobiotics (SIDOROV et al, 2001;NEPOMUCENO, 2009;COSTA et al, 2011;FURTADO;NEPOMUCENO, 2012;ORSOLIN et al, 2012). This test uses the strain of D. melanogaster having the marker wts (Warts) gene, that when expressed in the wild condition acts as a tumor suppressor gene (XU et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Assessment of carcinogenic potential of soft drinks of cola, diet cola, orange and lemon, produced in the city of Uberlândia, Minas Gerais state, Brazil

et al. 2016

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Soft drinks are industrialized unfermented beverages, free of alcohol, carbonated, rich in artificial flavors and sugar. The intense consumption of such beverages can be related to not inheritable diseases such as caries, allergy, cellulite and stretch marks, gastrointestinal disorders, diabetes and cancer. The aim of this study was to evaluate the carcinogenic potential of different concentrations of soft drinks produced in the Uberlândia city, Minas Gerais State, Brazil, by means of Epithelial Tumor Detection Test using Drosophila melanogaster as a model. Third stage larvae descendants of crosses between D. melanogaster virgin females wts/TM3, sb¹ and males mwh/mwh were treated with different concentrations (0.83, 1.66 or 3.33 mL/g) of cola, diet cola, orange or lemon soft drinks. The total epithelial tumor rate observed in flies treated with 3.33 mL/g of cola and orange soft drinks was higher than the negative control. The diet cola and lemon caused no significant increase in the overall frequency of epithelial tumors in D. melanogaster. In conclusion, in these experimental conditions, the cola and orange base soft drinks demonstrated carcinogenic potential in somatic cells of D. melanogaster in the concentration of 3.33 mL/g.

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“…For example, teratogenic effects of AFB 1 were observed on fetuses of hamster, mice, rat and cow by Aleksandrowicz and Smyk (1973). These teratogenic effects were also found as follows: decreases in body size and wing length (Lalor et al, 1976), formation of tumor on different body parts (Sidorov et al, 2001), hepatocarcinogen effects at vertebrates (Pier, 1981). These findings are in accordance with our results.…”

Section: Resultsmentioning

confidence: 99%

Selenium protective activity against aflatoxin B1 adverse affects on Drosophila melanogaster

Uysal

Ağar

2005

Braz. arch. biol. technol.

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Induction of tumor clones in D. melanogaster wts/+ heterozygotes with chemical carcinogens

Cited by 28 publications

References 14 publications

Lapachol as an epithelial tumor inhibitor agent in Drosophila melanogaster heterozygote for tumor suppressor gene wts

Lapachol as an epithelial tumor inhibitor agent in Drosophila melanogaster heterozygote for tumor suppressor gene wts

Assessment of carcinogenic potential of soft drinks of cola, diet cola, orange and lemon, produced in the city of Uberlândia, Minas Gerais state, Brazil

Selenium protective activity against aflatoxin B1 adverse affects on Drosophila melanogaster

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