Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide

Kaburaki, Yoichiro; Fujimura, Takashi; Kurata, Kentaro; Masuda, Kenichi; Toda, Masako; Yasueda, Hiroshi; Chida, Kazuhiro; Kawarai, Shinpei; Sakaguchi, Masahiro

doi:10.1016/j.cimid.2010.06.005

Cited by 13 publications

(12 citation statements)

References 26 publications

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“…In a clinical trial for ragweed allergy, peripheral DCs isolated from healthy individuals vaccinated with ragweed allergen conjugated to immunostimulatory oligodeoxyribonucleotide 1018 (Dynavax Technologies, Berkeley, CA) expressed increased levels of Th1 cytokines and decreased levels of Th2 cytokines (26). In a similar murine study, subcutaneous immunization of Balb/c mice with CpG conjugated to cedar pollen allergen was shown to increase the production of allergen-specific IgG2a and secretion of IFN-γ by CD4+ T cells isolated from spleens (27). With the clear demonstration of the importance of CpG at inducing a robust immunity against allergens, these studies also demonstrated that co-delivery of allergen with CpG is essential for stimulating an active Th1-type immune response (28).…”

Section: Introductionmentioning

confidence: 85%

Development of a Poly (lactic-co-glycolic acid) Particle Vaccine to Protect Against House Dust Mite Induced Allergy

Joshi

Adamcakova‐Dodd

Jing

et al. 2014

AAPS J

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Abstract. Poly(lactic-co-glycolic acid) (PLGA) particles carrying antigen and adjuvant is a promising vaccine system which has been shown to stimulate systemic antigen-specific immune responses. In this study, we investigated the relationship of (i) the sizes of PLGA particle and (ii) the presence of cytosinephosphate-guanine motifs (CpG), with the extent and type of immune response stimulated against Dermatophagoides pteronyssinus-2 (Der p2) antigen. Different sizes of PLGA particles encapsulating CpG were prepared using a double emulsion solvent evaporation method. Mice were vaccinated with Der p2 and different sizes of empty or CpG-loaded PLGA particles. Vaccinated mice were exposed to daily intranasal instillation of Der p2 for 10 days followed by euthanization to estimate leukocyte accumulation in bronchoalveolar lavage (BAL) fluids, antibody profiles, and airway hyperresponsiveness. PLGA particles showed a size-dependent decrease in the proportion of eosinophils found in BAL fluids. Mice vaccinated with the Der p2 coated on 9-μm-sized empty PLGA particles showed increased levels of IgE and IgG1 antibodies as well as increased airway hyperresponsiveness. All sizes of PLGA particles encapsulating CpG prevented airway hyperresponsiveness after Der p2 exposures. Inflammatory responses to Der p2 exposure were significantly reduced when smaller PLGA particles were used for vaccination. In addition, encapsulating CpG in PLGA particles increased IgG2a secretion. This study shows that the size of PLGA particles used for vaccination plays a major role in the prevention of house dust mite-induced allergy and that incorporation of CpG into the PLGA particles preferentially develops a Th1-type immune response.

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Section: Introductionmentioning

confidence: 85%

Development of a Poly (lactic-co-glycolic acid) Particle Vaccine to Protect Against House Dust Mite Induced Allergy

Joshi

Adamcakova‐Dodd

Jing

et al. 2014

AAPS J

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“…On the contrary, CpG-containing immunostimulatory oligodeoxinucleotides (ODNs) have shown to be able to generate Th1 responses through activation of TLR-9 receptors [14] and may be a better adjuvant choice for SIT. CpG ODNs have proven their efficacy as both a therapeutic alternative and as an adjuvant in mouse models of allergic rhinitis and asthma, acting as inductors of Th1 responses and inhibitors of allergen-induced airway hyperresponsiveness, eosinophilia as well as airway remodelling [15,16,17,18,19]. …”

Section: Introductionmentioning

confidence: 99%

Modulation of the Humoral Response to <i>Dermatophagoides pteronyssinus </i>Allergens in BALB/c Mice by Extract Modification and Adjuvant Use

Coaña

Carnés²,

Gallego³

et al. 2011

Int Arch Allergy Immunol

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Background: Currently, several strategies are being used in order to improve the safety and efficacy of allergen-specific immunotherapy; these strategies include the use of modified hypoallergenic extracts as well as different adjuvants with immunomodulatory properties in combination with native or modified extracts. The objectives of this study were to investigate the humoral response generated in mice to modified Dermatophagoides pteronyssinus extracts in the presence or absence of two different adjuvants. Methods: BALB/c mice were inoculated either with native, depigmented or depigmented-polymerised D. pteronyssinus without adjuvants or combined with aluminium hydroxide or oligodeoxinucleotides containing CpG motifs. IgE concentration, specific total IgG, IgG1 and IgG2a titres were measured in mice sera and cross-reactivity inhibition experiments were performed. IgG antigenic profiles were obtained by immunoblotting for all formulations. Results: Inoculation of depigmented-polymerised extract induced statistically significant lower IgE levels than the native extract even when adsorbed onto aluminium hydroxide. When this extract was inoculated in the presence of oligodeoxinucleotides containing CpG motifs, it elicited high IgG levels, a high IgG2a/lgG1 ratio and low IgE production. Furthermore, the antigenic profiles observed after extract inoculation showed punctual differences between the depigmented-polymerised extract and the native or depigmented extracts. Conclusions: Our results suggest that the depigmentation and polymerisation process modifies the native extract’s antigenic and immunogenic properties and converts the depigmented-polymerised extract into a better choice for allergen-specific immunotherapy.

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“…In this study, an increase of IFN-γ, IgG2a, and IgG2b was not observed with the Cryj2 T-cell epitope peptide and CpG-B ODN conjugate, but an increase in IFN-γ, IgG2a, and IgG2b was found in studies on a conjugate injection of another cedar pollen allergen Cryj1 and CpG-B ODN, which demonstrated activation of Th1-biased responses. 87 In addition to the high cost associated with the chemical conjugation of CpG ODNs to antigens or allergens, the immunostimulatory properties may be changed through structural changes in the antigen by the synthesis process. 88 Shirota and Klinman 89 bonded amino-modified CpG-B ODNs to proteins on the surface of apoptotic tumor cells using the water-soluble cross-linking agent bis(sulfosuccinimidyl) suberate.…”

mentioning

confidence: 99%

CpG oligodeoxynucleotide nanomedicines for the prophylaxis or treatment of cancers, infectious diseases, and allergies

Hanagata

2017

IJN

110

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Induction of Th1 immune responses to Japanese cedar pollen allergen (Cry j 1) in mice immunized with Cry j 1 conjugated with CpG oligodeoxynucleotide

Cited by 13 publications

References 26 publications

Development of a Poly (lactic-co-glycolic acid) Particle Vaccine to Protect Against House Dust Mite Induced Allergy

Development of a Poly (lactic-co-glycolic acid) Particle Vaccine to Protect Against House Dust Mite Induced Allergy

Modulation of the Humoral Response to <i>Dermatophagoides pteronyssinus </i>Allergens in BALB/c Mice by Extract Modification and Adjuvant Use

CpG oligodeoxynucleotide nanomedicines for the prophylaxis or treatment of cancers, infectious diseases, and allergies

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