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“…Mitogenic activation of T cells associated with cytokine expression and secretion is one of the best-known activities of Con A (21). Such a proliferative response is generally thought to be due to receptor cross-linking, but has also been observed with monomeric or dimeric Con A species (3,4).…”
mentioning
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“…Mitogenic activation of T cells associated with cytokine expression and secretion is one of the best-known activities of Con A (21). Such a proliferative response is generally thought to be due to receptor cross-linking, but has also been observed with monomeric or dimeric Con A species (3,4).…”
mentioning
“…4). It has been published before that monomeric Con A is still biologically active, although in particular the capacity to induce IL 2 production is reduced compared to the tetrameric configuration [28]. An alternative explanation is that the monomeric Con A reaggregates to the multimeric form after gel filtration.…”
Section: Induction Of T Cell Helper Activity By Con a And Il2mentioning
“…These data show that low doses of Con A can be An unexpected finding was the detection with specific mAb of a large amount of Con A in the CM preparations. Con A is a tetrameric molecule of 105 kDa [28] which elutes on an AcA-54 column at a different positition compared to IL2. Therefore, it was unlikely to be present in CM-derived material of approximately 20 kDa.…”
Section: Induction Of T Cell Helper Activity By Con a And Il2mentioning