Induction of Somatic Embryogenesis in Sengon ( Falcataria moluccana ) With Thidiazuron and Light Treatments

Sunandar, Ari; Dorly,; Supena, Ence Darmo Jaya

doi:10.1016/j.hjb.2017.08.002

Cited by 12 publications

(10 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It may be that light exposure is beneficial to SE only after spherical embryo formation. Similarly, in a study of Sengon (Falcataria moluccana (Miquel) Barneby & Grimes), dark treatment followed by light treatment was reported to be beneficial to SE [52]. However, different results were reported in a study on strawberry trees [53], in which light inhibited callus formation and SE, and similar results were also obtained in Larix kaempferi Sarg.…”

Section: Other Factors Influencing Somatic Embryogenesismentioning

confidence: 75%

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

Yang

Guo

et al. 2018

Forests

View full text Add to dashboard Cite

Research Highlights: In the current study, we established a method for plant regeneration via somatic embryogenesis (SE) in Koelreuteria paniculata Laxm. for the first time. Background and Objectives: K. paniculata is an important ornamental and medicinal plant in China. However, the plant has difficulty with asexual reproduction, which imposes a limitation on large-scale propagation. Materials and Methods: Embryogenic calluses were induced from stems of aseptic seedlings on induction media. The effects of different media types and concentrations of N6-benzyladenine (BA), α-naphthaleneacetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction were examined. Embryogenic calluses were then transferred to Driver-Kuniyuki Walnut (DKW) media containing NAA (0.1–0.2 mg L−1) or 2,4-D (0.5–2.0 mg L−1) to develop somatic embryos. Cotyledon embryos were cultured on DKW media containing NAA (0.1–0.2 mg L−1) until maturation, and were then transferred to 1/2 DKW medium supplemented with 1.0 mg L−1 indole-3-butyric acid (IBA) to produce complete plants. The effects of IBA and NAA on rhizogenesis were then examined by clonal culture. Results: The maximum callus induction frequency (80.25%) was obtained on DKW medium supplemented by 0.5 mg L−1 BA, 0.25 mg L−1 NAA, and 1.5 mg L−1 2,4-D. NAA had a more pronounced effect on somatic embryo growth than did 2,4-D, with a maximum SE frequency (54.75%) observed with 0.1 mg L−1 NAA added to DKW medium. For clonal culture, the highest rooting rate (52%) was observed on 1/4 DKW medium containing 1.5 mg L−1 IBA. Histology studies confirmed the presence of embryogenic calluses and somatic embryos in different stages. Conclusions: This protocol provides a novel method for large-scale propagation of K. paniculata, and creates opportunities for genetic engineering in this species.

show abstract

Section: Other Factors Influencing Somatic Embryogenesismentioning

confidence: 75%

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

Yang

Guo

et al. 2018

Forests

View full text Add to dashboard Cite

show abstract

“…Successful induction of somatic embryo in light or dark condition was different among plants. Somatic embryogenesis can be achieved in dark condition (Tahardi et al, 2000;Gomes et al, 2006), dark condition followed by light treatment (Sunandar et al, 2017), or fully in light condition (de-la-Pena et al, 2008;Bakhshaie et al, 2010;Lema-Ruminska & Kulus, 2012). In tea micropropagation, light was also important to induce somatic embryogenesis (Akula et al, 2000, Akula & Dodd, 1998 and were in confirmation of the present results of successful induction of somatic embryogenesis when explants were culture on light condition.…”

Section: Introductionmentioning

confidence: 99%

Induction of Indirect Somatic Embryogenesis on Embryonic Axis of TRI2025 Tea Clone

Eskundari¹,

Taryono²,

Indradewa³

et al. 2018

JAS

View full text Add to dashboard Cite

Tea (Camellia sinensis L.) is classified as cross-pollinated crop and vegetative multiplication becomes commercially the main method of propagation with some limitations such as high heterogeneity and poor in survival rate and also in rooting. A proven tissue culture method, somatic embryogenesis, is the only challenging way to meet the needs of tea seedlings in large quantities. The study was conducted with TRI2025 tea clone selected from Polyclonal garden of PT. Pagilaran (Batang, Central Java). The explants were cultured on MS media supplemented with four concentrations of 2,4-D (0, 1, 2, and 5 mg L-1) in two incubation conditions; dark and light. The results showed the only concentration of 2,4-D that can induce somatic embryo was 2 mg L-1 2,4-D in light condition and its percentage was about 5%. Other concentrations of 2,4-D that given for treatments both in two conditions will not induce somatic embryo. This study needs more improvements for getting powerful and efficient of method to get somatic embryo-derived plant and also for futher successful genetic engineering of tea biotechnology.

show abstract

“…Auxin is often required for the induction of somatic embryogenesis from different explants, and the process of somatic embryogenesis is generally initiated on the culture medium supplemented with high concentrations of auxins [ 11 ]. However, somatic embryogenesis has been induced on auxin-free media in various plants [ 12 – 14 ]. In several plants, a combination of auxin and cytokinin stimulates the development of somatic embryogenesis [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…and Endl.) H. Robinson] [ 15 ].The sensitivity of the explant tissue to the PGRs may have been altered by the dark treatment, thereby resulting in a higher frequency of embryogenesis [ 12 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Dehestani-Ardakani¹,

Hejazi²,

Aliabad

2020

Mol Biol Rep

View full text Add to dashboard Cite

Purple coneflower (Echinacea purpurea (L.) Moench) is a widely used medicinal and ornamental plant. In the present study, the callus embryogenesis was examined using benzyl adenine (BA) at three levels (3, 4, 5 mg L−1), 1-Naphthalene acetic acid (NAA) at three levels (0.1, 0.2 and 0.5 mg L−1) with or without activated charcoal (1 g L−1), coconut milk (50 ml L−1) and casein hydrolysate (50 mg L−1) in the MS (Murashige and Skoog 1962) medium. The embryogenesis indirectly occurred with the production of callus. The calli were observed in three forms: undifferentiated, embryogenic and organogenic. The embryogenic calli were dark green and coherent with a faster growth rate. The highest embryogenesis (100%) and embryonic regeneration (plantlet production) were obtained in the combined BA + NAA treatments with the activated charcoal, coconut milk and casein hydrolysate. However, the combined treatments of growth regulators failed to produce somatic embryos without the use of coconut milk and casein hydrolysate. The maximum amount of protein, peroxidase and catalase activity of embryogenic calli (2.02, 1.79 and 6.62ΔOD/Min/mg.protein, respectively), and highest percentage of acclimatization success (29.3% of plants) were obtained in the combined treatment of 5 mg L−1 BA + 0.5 mg L−1 NAA + activated charcoal + coconut milk + casein hydrolysate. The highest amount of chlorophyll content (33.3 SPAD value) and growth characteristics of acclimatized plantlets were observed in the media containing 3 mg L−1 BA + 0.1 and 0.2 mg L−1 NAA + 1 g. L−1 combined activated charcoal, coconut milk, casein hydrolysate. The histological studies confirmed the somatic embryogenesis in purple coneflower. Generally, it was found that the somatic embryogenesis of E. purpurea occurs at high levels of BA and low levels of NAA with the addition of coconut milk and casein hydrolysate.

show abstract

Induction of Somatic Embryogenesis in Sengon ( Falcataria moluccana ) With Thidiazuron and Light Treatments

Cited by 12 publications

References 16 publications

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

High-Efficiency Somatic Embryogenesis from Seedlings of Koelreuteria paniculata Laxm.

Induction of Indirect Somatic Embryogenesis on Embryonic Axis of TRI2025 Tea Clone

Indirect somatic embryogenesis of purple coneflower (Echinacea purpurea (L.) Moench): a medicinal-ornamental plant: evaluation of antioxidant enzymes activity and histological study

Contact Info

Product

Resources

About