Abstract:In this study, we designed an experiment to predict a potential
immunodominant T-cell epitope and evaluate the protectivity of this antigen in
immunised mice. The T-cell epitopes of the candidate proteins (EgGST, EgA31,
Eg95, EgTrp and P14-3-3) were detected using available web-based databases. The
synthesised DNA was subcloned into the pET41a+ vector and expressed in
Escherichia coli as a fusion to glutathione-S-transferase
protein (GST). The resulting chimeric protein was then purified by affinity
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