Induction of metallothionein synthesis by zinc in cadmium pretreated rats

Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism

“…Additional studies are needed to evaluate this hypothesis. Suzuki et al [49] showed that co-treatment with Cd 2+ and Zn 2+ salts might increase synthesis of MT in different organs, including the liver, kidneys, and pancreas, relative to that reported for treatment with Cd 2+ or Zn 2+ alone. When cells are exposed to Cd 2+ and Zn 2+ simultaneously, both metals compete for MT binding sites [7].…”

Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism

“…Additional studies are needed to evaluate this hypothesis. Suzuki et al [49] showed that co-treatment with Cd 2+ and Zn 2+ salts might increase synthesis of MT in different organs, including the liver, kidneys, and pancreas, relative to that reported for treatment with Cd 2+ or Zn 2+ alone. When cells are exposed to Cd 2+ and Zn 2+ simultaneously, both metals compete for MT binding sites [7].…”

A calcium channel blocker nifedipine distorts the effects of nano-zinc oxide on metal metabolism in the marsh frog Pelophylax ridibundus

“…It has been proposed that MTs play an important role in Zn homeostasis by controlling cellular Zn uptake, distribution, storage, and release (Maret, 2011, Kimura and Kambe, 2016). Moreover, Zn can stimulate MTs synthesis (Suzuki et al, 1990). Despite Zn is a weaker inductor of MTs than cadmium (Onosaka et al, 1984), numerous investigations have reported that Zn should affect MTs concentration and their mRNA synthesis in various species, among them freshwater vertebrate.…”

Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry

“…When the cells are exposed to Cd, MT induced in the cells binds not only Cd but also Zn. 15 However, Zn could not be detected on the second dimensional micro HPLC column since the detection limit of Zn is higher than that of Cd, furthermore the stable isotope enrichment was not applied to the Zn detection in this study. Cu could not be also detected on the 2D column because the amount of Cu bound to MT was not increased by the Cd treatment.…”

Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism

“…Additional studies are needed to evaluate this hypothesis. Suzuki et al [49] showed that co-treatment with Cd 2+ and Zn 2+ salts might increase synthesis of MT in different organs, including the liver, kidneys, and pancreas, relative to that reported for treatment with Cd 2+ or Zn 2+ alone. When cells are exposed to Cd 2+ and Zn 2+ simultaneously, both metals compete for MT binding sites [7].…”

A calcium channel blocker nifedipine distorts the effects of nano-zinc oxide on metal metabolism in the marsh frog Pelophylax ridibundus

“…It has been proposed that MTs play an important role in Zn homeostasis by controlling cellular Zn uptake, distribution, storage, and release (Maret, 2011, Kimura and Kambe, 2016). Moreover, Zn can stimulate MTs synthesis (Suzuki et al, 1990). Despite Zn is a weaker inductor of MTs than cadmium (Onosaka et al, 1984), numerous investigations have reported that Zn should affect MTs concentration and their mRNA synthesis in various species, among them freshwater vertebrate.…”

Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry

“…When the cells are exposed to Cd, MT induced in the cells binds not only Cd but also Zn. 15 However, Zn could not be detected on the second dimensional micro HPLC column since the detection limit of Zn is higher than that of Cd, furthermore the stable isotope enrichment was not applied to the Zn detection in this study. Cu could not be also detected on the 2D column because the amount of Cu bound to MT was not increased by the Cd treatment.…”

Cytotoxic effects of cadmium and zinc co-exposure in PC12 cells and the underlying mechanism

“…Additional studies are needed to evaluate this hypothesis. Suzuki et al [49] showed that co-treatment with Cd 2+ and Zn 2+ salts might increase synthesis of MT in different organs, including the liver, kidneys, and pancreas, relative to that reported for treatment with Cd 2+ or Zn 2+ alone. When cells are exposed to Cd 2+ and Zn 2+ simultaneously, both metals compete for MT binding sites [7].…”

A calcium channel blocker nifedipine distorts the effects of nano-zinc oxide on metal metabolism in the marsh frog Pelophylax ridibundus

“…It has been proposed that MTs play an important role in Zn homeostasis by controlling cellular Zn uptake, distribution, storage, and release (Maret, 2011, Kimura and Kambe, 2016). Moreover, Zn can stimulate MTs synthesis (Suzuki et al, 1990). Despite Zn is a weaker inductor of MTs than cadmium (Onosaka et al, 1984), numerous investigations have reported that Zn should affect MTs concentration and their mRNA synthesis in various species, among them freshwater vertebrate.…”

Separation of metallothionein isoforms extracted from isoform-specific knockdown cells on two-dimensional micro high-performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry

“…When the cells are exposed to Cd, MT induced in the cells binds not only Cd but also Zn. 15 However, Zn could not be detected on the second dimensional micro HPLC column since the detection limit of Zn is higher than that of Cd, furthermore the stable isotope enrichment was not applied to the Zn detection in this study. Cu could not be also detected on the 2D column because the amount of Cu bound to MT was not increased by the Cd treatment.…”