2019
DOI: 10.1167/iovs.19-26983
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Abstract: Induction of fibroblast senescence during mouse corneal wound healing. Invest Ophthalmol Vis Sci. 2019;60:3669-3679. https://doi.org/10.1167/iovs.19-26983 PURPOSE. To investigate the presence and role of fibroblast senescence in the dynamic process of corneal wound healing involving stromal cell apoptosis, proliferation, and differentiation.METHODS. An in vivo corneal wound healing model was performed using epithelial debridement in C57BL/6 mice. The corneas were stained using TUNEL, Ki67, and a-smooth mus… Show more

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Cited by 35 publications
(20 citation statements)
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“…As previously discussed, SA-b-gal has allowed the development of fluorogenic compounds functionalized as substrates that allow the labelling of senescent cells when cleaved by this enzymatic activity. These include C 12 FGD [45] and SPiDER-bgal [106], which have been successfully used to detect b-gal high -expressing cells in live tissues [107][108][109]. However, for greater confidence, these are recommended to be assayed in conjunction with other markers that can be detected simultaneously using additional filters.…”
Section: Flow Cytometrymentioning
confidence: 99%
“…As previously discussed, SA-b-gal has allowed the development of fluorogenic compounds functionalized as substrates that allow the labelling of senescent cells when cleaved by this enzymatic activity. These include C 12 FGD [45] and SPiDER-bgal [106], which have been successfully used to detect b-gal high -expressing cells in live tissues [107][108][109]. However, for greater confidence, these are recommended to be assayed in conjunction with other markers that can be detected simultaneously using additional filters.…”
Section: Flow Cytometrymentioning
confidence: 99%
“…The corneal epithelium is the first protective barrier of eyes against physical trauma, pathogens, and chemicals, and it contributes to the maintenance of corneal transparency and rigidity. Corneal epithelial wound healing is a complex process involving cell apoptosis, proliferation, migration, senescence, and differentiation (Ljubimov & Saghizadeh, 2015;X. Wang et al, 2019).…”
Section: Introductionmentioning
confidence: 99%
“…6 The triggering of apoptotic cascades generally involved p21, a cyclin-dependent kinase inhibitor, mediator of p53-dependent cell cycle arrest, and inducer of G1/S and G2/M phase arrest. ROS-mediated upregulation of p53 28,41,120 and p21 28,45,55,121124 was indeed observed in several cell types, even only 2 h after administration. 122 Interestingly, a study using rat mouse gingival fibroblasts showed that treatment with 20 µM hydrogen peroxide increased cell proliferation and phosphorylation of the p53 at the same time.…”
Section: Cell Signaling Activated By Rosmentioning
confidence: 89%