Induction of DNA double-strand breaks in mammalian cells and yeast

Frankenberg, D.; Brede, H.J.; Schrewe, U.J.; Steinmetz, Carolin; Frankenberg-Schwager, M.; Kasten, G.; Pralle, E.

doi:10.1016/s0273-1177(99)01066-2

Cited by 16 publications

(5 citation statements)

References 27 publications

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“…Moreover, although X-rays are considered low-linear energy transfer (LET) radiation [2], a higher relative biological effectiveness (RBE) at very low-energy (≤30 keV) than at high energy has been reported in the literature. Indeed, very low energy X-rays may lead to higher risk estimates at low doses for many biological endpoints such as double-strand breaks (DSBs), chromosome aberrations, micronucleus formation, and cell survival [3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22]. This could be explained by the fact that energy depositions vary between these different photon energies since the type of interaction (photoelectric effect, Compton effect, or pair production), which causes the energy deposition depends on the photon energy [23].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

Tang

Bueno

Meylan³

et al. 2019

IJMS

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The objective of this work was to study the differences in terms of early biological effects that might exist between different X-rays energies by using a mechanistic approach. To this end, radiobiological experiments exposing cell monolayers to three X-ray energies were performed in order to assess the yields of early DNA damage, in particular of double-strand breaks (DSBs). The simulation of these irradiations was set in order to understand the differences in the obtained experimental results. Hence, simulated results in terms of microdosimetric spectra and early DSB induction were analyzed and compared to the experimental data. Human umbilical vein endothelial cells (HUVECs) were irradiated with 40, 220 kVp, and 4 MV X-rays. The Geant4 Monte Carlo simulation toolkit and its extension Geant4-DNA were used for the simulations. Microdosimetric calculations aiming to determine possible differences in the variability of the energy absorbed by the irradiated cell population for those photon spectra were performed on 10,000 endothelial cell nuclei representing a cell monolayer. Nanodosimetric simulations were also carried out using a computation chain that allowed the simulation of physical, physico-chemical, and chemical stages on a single realistic endothelial cell nucleus model including both heterochromatin and euchromatin. DNA damage was scored in terms of yields of prompt DSBs per Gray (Gy) and per giga (109) base pair (Gbp) and DSB complexity was derived in order to be compared to experimental data expressed as numbers of histone variant H2AX (γ-H2AX) foci per cell. The calculated microdosimetric spread in the irradiated cell population was similar when comparing between 40 and 220 kVp X-rays and higher when comparing with 4 MV X-rays. Simulated yields of induced DSB/Gy/Gbp were found to be equivalent to those for 40 and 220 kVp but larger than those for 4 MV, resulting in a relative biological effectiveness (RBE) of 1.3. Additionally, DSB complexity was similar between the considered photon spectra. Simulated results were in good agreement with experimental data obtained by IRSN (Institut de radioprotection et de sûreté nucléaire) radiobiologists. Despite differences in photon energy, few differences were observed when comparing between 40 and 220 kVp X-rays in microdosimetric and nanodosimetric calculations. Nevertheless, variations were observed when comparing between 40/220 kVp and 4 MV X-rays. Thanks to the simulation results, these variations were able to be explained by the differences in the production of secondary electrons with energies below 10 keV.

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Section: Introductionmentioning

confidence: 99%

Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

Tang

Bueno

Meylan³

et al. 2019

IJMS

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“…This problem has received great attention from many researchers in the radiation biology and radiation physics areas. Three main approaches have been used to investigate the enhanced effectiveness of low-energy photons compared to γ -rays: in vitro radiobiological studies (see the review in Prise et al (1998), Frankenberg et al (2000)), epidemiological studies (see the review in Hunter and Muirhead (2009)) and Monte Carlo (MC) simulations of the radiation-DNA interaction , Pomplun 1991, Friedland et al 1998, 1999. Many biological studies related to the RBE of mammography beams have been published during the last decade (Frankenberg et al 2002, Kühne et al 2005, Lehnert et al 2008, Mestres et al 2009, Schmid et al 2002.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the RBE of mammography-quality beams using a combination of a Monte Carlo code with a B-DNA geometrical model

Bernal¹,

deAlmeida

David

et al. 2011

Phys. Med. Biol.

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The PENELOPE code is used to determine direct strand break yields corresponding to photons from a (60)Co source and 28 and 30 kV x-ray beams impacting on a B-DNA geometrical model, which accounts for five organizational levels of the human genetic material. Direct single, double and total strand break probabilities are determined in a liquid water homogeneous medium with 1.06 g cm(-3) density. The spectra produced by the x-ray beams at various depths in the phantom have been used to study the dependence of the damage yield on the depth. The relative biological effectiveness (RBE) is also estimated using the (60)Co radiation qualities as the reference. According to this work, the damage probabilities and thus the RBE are, within the uncertainties, similar for both x-ray energies and are independent of the depth into the phantom. Furthermore, the total strand break yield is invariant with respect to the energy of the incident photons. The RBE for low-energy x-ray beams determined here (1.3 ± 0.1) is lower than that reported by Kellerer, taking into account that he used a 200 kV radiation as the reference quality. However, our RBE values are consistent with those determined by Kühne et al (2005 Radiat. Res. 164 669-76), which used the same biological endpoint and reference quality as our study. Also, our RBE values are similar to those determined by Verhaegen and Reniers (2004 Radiat. Res. 162 592-9).

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“…RBE is usually defined as the ratio of the dose of a low LET reference radiation to the dose of another radiation needed to achieve the same biological effect. Because the induction of DNA damage is proportional to the absorbed dose D, up to at least a few hundred Gy of low and high LET radiation [37][38][39], RBE can also be defined as the ratio of the damaged yields of cells irradiated with different radiation sources, as shown below:…”

Section: E Relative Biological Effectiveness (Rbe)mentioning

confidence: 99%

A Computational Method to Estimate the Effect of Gold Nanoparticles on X-Ray Induced Dose Enhancement and Double-Strand Break Yields

Hsiao¹,

Tai²,

Chan³

et al. 2021

IEEE Access

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We report the effects of gold nanoparticles (GNP) on the enhancement of the dose effect and double-strand break (DSB) induction for X-ray monoenergies (10 keV-2 MeV). The overall relative biological effectiveness (RBE) was defined as the product of dose and DSB enhancement. The cellular doses and energy spectra were computed using the PENELOPE (PENetration and Energy LOss of Positrons and Electrons) code. The DNA damage yields were estimated using the MCDS (Monte Carlo damage simulation) code. Considering the production of Auger electrons induced by 7mg/ml GNP, we found that dose enhancement at the 30 keV was ~4.0 for cells with radius 4 μm. The DSB induction increased as GNP concentrations increased and was dependent on X-ray energy. These findings demonstrated that the maximum RBE was attained at 30-40 keV and therefore this energy range would be more efficient in cancer cell killing. INDEX TERMS Relative biological effectiveness, Gold nanoparticle 8Ya-Yun Hsiao received the Diploma degree in physics from the National Tsing-

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Induction of DNA double-strand breaks in mammalian cells and yeast

Cited by 16 publications

References 27 publications

Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

Assessment of Radio-Induced Damage in Endothelial Cells Irradiated with 40 kVp, 220 kVp, and 4 MV X-rays by Means of Micro and Nanodosimetric Calculations

Estimation of the RBE of mammography-quality beams using a combination of a Monte Carlo code with a B-DNA geometrical model

A Computational Method to Estimate the Effect of Gold Nanoparticles on X-Ray Induced Dose Enhancement and Double-Strand Break Yields

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