1998
DOI: 10.1128/jb.180.19.5144-5150.1998
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Induction of Ca 2+ -Calmodulin Signaling by Hard-Surface Contact Primes Colletotrichum gloeosporioides Conidia To Germinate and Form Appressoria

Abstract: Hard-surface contact primes the conidia of Colletotrichum gloeosporioides to respond to plant surface waxes and a fruit-ripening hormone, ethylene, to germinate and form the appressoria required for infection of the host. Our efforts to elucidate the molecular events in the early phase of the hard-surface contact found that EGTA (5 mM) and U73122 (16 nM), an inhibitor of phospholipase C, inhibited (50%) germination and appressorium formation. Measurements of calmodulin (CaM) transcripts with a CaM cDNA we clon… Show more

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Cited by 101 publications
(61 citation statements)
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References 36 publications
(46 reference statements)
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“…A high level of transcript accumulation of CgCaM was observed in appressorium-forming conidia of C. gloeosporioides in this study. Similar transcriptional activation of calmodulin genes has been reported in M. grisea [21], C. gloeosporioides virulent on avocado [4], and C. trifolii [6]. Spermidine treatment suppressed not only prepenetration development but also CgCaM expression in this study.…”
Section: Discussionsupporting
confidence: 89%
See 1 more Smart Citation
“…A high level of transcript accumulation of CgCaM was observed in appressorium-forming conidia of C. gloeosporioides in this study. Similar transcriptional activation of calmodulin genes has been reported in M. grisea [21], C. gloeosporioides virulent on avocado [4], and C. trifolii [6]. Spermidine treatment suppressed not only prepenetration development but also CgCaM expression in this study.…”
Section: Discussionsupporting
confidence: 89%
“…We previously demonstrated that prepenetration development of C. gloeosporioides pathogenic on red pepper is under the control of calcium/calmodulin-dependent signaling pathway [5]. Similar results were also presented in C. gloeosporioides infecting avocado [4] and C. trifolii [3]. Exogenous addition of calcium completely restored conidial germination and appressorium formation interdicted by polyamines and MGBG.…”
Section: Discussionsupporting
confidence: 77%
“…Our previous data [13] showed that CAM inhibited not only appressorial formation, but also germination. Similar conclusions were drawn by Kim et al for C. gloeosporioides [11]. Why spore germination of AS-1 and AS-2 was una¡ected is not clear, but it could be due to residual CAM levels prior to QA induction, resulting in su¤cient levels of CAM to drive the germination process.…”
Section: Discussionsupporting
confidence: 80%
“…Pieterse et al [10] isolated CAM from Phytophthora infestans in a screen designed to identify fungal genes induced by host during disease interactions. In Colletotrichum gloeosporioides, CAM expression is triggered by surface contact [11].…”
Section: Introductionmentioning
confidence: 99%
“…Calcium signaling appears to be nonessential in the early stages of conidial germination in A. nidulans. Deletion or delayed expression of the Ca 2 /calmodulin-dependent kinases slows entry into the cell cycle but does not completely block germination [33]. In M. grisea and C. trifolii, appressorium formation, but not germination, is completely blocked in the presence of calcium chelators, ionophores and calcium regulators [34,35].…”
Section: Calcium Signaling During Conidial Germinationmentioning
confidence: 99%