Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene

Yuen, Bonny; Wong, Chris K C; Woo, Norman Y.S.; Au, Doris W.T.

doi:10.1016/j.aquatox.2007.02.010

Cited by 37 publications

(18 citation statements)

References 40 publications

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“…Considering the great capacity of liver regeneration [46], [47], these terms suggest, despite the adverse effects of BaP and Phe-exposure, an activation of signaling pathway leading to cell turnover, resulting in hepatic tissue remodeling or regeneration affected by xenobiotic compounds. Cell turnover was also detected in the epithelial intestine cell of the species Epinephelus coioides after one week and also after four weeks of exposure to BaP [48]. Furthermore, these authors, as well as our study, did not found an increase of apoptotic process between the treatments and the controls.…”

Section: Discussionsupporting

confidence: 80%

Functional Categorization of Transcriptome in the Species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) Exposed to Benzo[a]pyrene and Phenanthrene

et al. 2013

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This study aims to evaluate the transcriptome alterations, through cDNA libraries, associated with the combined effects of two PAHs, benzo[a]pyrene (0.5 µg/L) and phenanthrene (50 µg/L), present in crude oil, on specimens of Symphysodon aequifasciatus (discus fish) after 48 h of exposure. The cDNA libraries were constructed according to the SOLiD™ SAGE™ protocol for sequencing in the SOLiD v.3 Plus sequencer. The results were analyzed by bioinformatics and differentially expressed genes were categorized using the gene ontology program. The functional categories (terms) found in the gene ontology and the gene network generated using STRING software were used to predict the adverse effects of benzo[a]pyrene and phenanthrene in the liver. In the present study, 27,127 genes (compared to Danio rerio database) were identified. Considering only those genes with a p-value less than or equal to 0.05 and greater than or equal to two-fold change in expression across libraries, we found 804 genes, 438 down-regulated (54%) and 366 up-regulated (46%), in the experimental group compared to the control. Out of this total, 327 genes were successfully categorized, 174 down-regulated and 153 up-regulated, using gene ontology. Using String, the gene network was composed by 199 nodes, 124 of them resulting in 274 interactions. The results showed that even an acute exposure of 48 h caused metabolic change in response to environmental contaminants, resulting in changes of cell integrity, in oxidation-reduction processes, in the immune response and disturbances of intracellular signaling of discus fish. Also the gene network has showed no central interplay cluster, exhibiting instead interconnected clusters interactions and connected sub-networks. These findings highlight that even an acute sublethal exposure of PAHs can cause metabolism changes that may affect survival of discus. Our findings using SOLiD coupled with SAGE-method resulted in a powerful and reliable means for gene expression analysis in discus, a non-model Amazonian fish.

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Section: Discussionsupporting

confidence: 80%

Functional Categorization of Transcriptome in the Species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) Exposed to Benzo[a]pyrene and Phenanthrene

et al. 2013

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“…High doses of PAH result in significant intestinal hyperplasia in fish, with an increase in cell proliferation and faster epithelial turnover [59]. Previous studies on intestinal gene expression in fish indicated a reduction in cell proliferation or differentiation associated with dietary FO replacement by VO [21,22], possibly due to lower levels of membrane LC-PUFA and reduced oxidative stress [22].…”

Section: Discussionmentioning

confidence: 99%

Effects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar)

et al. 2012

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BackgroundExpansion of aquaculture requires alternative feeds and breeding strategies to reduce dependency on fish oil (FO) and better utilization of dietary vegetable oil (VO). Despite the central role of intestine in maintaining body homeostasis and health, its molecular response to replacement of dietary FO by VO has been little investigated. This study employed transcriptomic and proteomic analyses to study effects of dietary VO in two family groups of Atlantic salmon selected for flesh lipid content, 'Lean' or 'Fat'.ResultsMetabolism, particularly of lipid and energy, was the functional category most affected by diet. Important effects were also measured in ribosomal proteins and signalling. The long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis pathway, assessed by fatty acid composition and gene expression, was influenced by genotype. Intestinal tissue contents of docosahexaenoic acid were equivalent in Lean salmon fed either a FO or VO diet and expression of LC-PUFA biosynthesis genes was up-regulated in VO-fed fish in Fat salmon. Dietary VO increased lipogenesis in Lean fish, assessed by expression of FAS, while no effect was observed on β-oxidation although transcripts of the mitochondrial respiratory chain were down-regulated, suggesting less active energetic metabolism in fish fed VO. In contrast, dietary VO up-regulated genes and proteins involved in detoxification, antioxidant defence and apoptosis, which could be associated with higher levels of polycyclic aromatic hydrocarbons in this diet. Regarding genotype, the following pathways were identified as being differentially affected: proteasomal proteolysis, response to oxidative and cellular stress (xenobiotic and oxidant metabolism and heat shock proteins), apoptosis and structural proteins particularly associated with tissue contractile properties. Genotype effects were accentuated by dietary VO.ConclusionsIntestinal metabolism was affected by diet and genotype. Lean fish may have higher responsiveness to low dietary n-3 LC-PUFA, up-regulating the biosynthetic pathway when fed dietary VO. As global aquaculture searches for alternative oils for feeds, this study alerts to the potential of VO introducing contaminants and demonstrates the detoxifying role of intestine. Finally, data indicate genotype-specific responses in the intestinal transcriptome and proteome to dietary VO, including possibly structural properties of the intestinal layer and defence against cellular stress, with Lean fish being more susceptible to diet-induced oxidative stress.

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“…These environmental stresses have been previously shown to impair fish growth (Al-Yakoob et al, 1996;Zhou et al, 2001;Bugeon et al, 2004;Weber and Bosworth, 2005;Stierhoff et al, 2006;Yuen et al, 2007). Muscle TA in fish is highly responsive to food availability: suppressed by reduced food intake and induced by re-feeding.…”

Section: Fish Muscle Telomerase Activity Is Responsive To Environmentmentioning

confidence: 98%

“…This concentration of foodborne B[a]P was chosen as it is environmentally realistic (Zhang et al, 2012), and has been found to (i) significantly increase muscle B[a]P body burden in juvenile areolated grouper (Epinephelus areolatus) (Wu et al, 2003) and (ii) inhibit growth in juvenile carnivorous fish (Epinephelus coioides) (Yuen et al, 2007). The detailed experimental steps were described by Yuen and Au (2006); Yuen et al (2007). After 1 month of B[a]P exposure, five fish were sampled randomly from both the solvent control and B[a]P treatment tanks.…”

Section: Experiments 3: Chronic Food-borne Exposure To B[a]pmentioning

confidence: 99%

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Modulation of telomerase activity in fish muscle by biological and environmental factors

Peterson

Mok

2015

Comparative Biochemistry and Physiology Part C: Toxicology &

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Induction and recovery of morphofunctional changes in the intestine of juvenile carnivorous fish (Epinephelus coioides) upon exposure to foodborne benzo[a]pyrene

Cited by 37 publications

References 40 publications

Functional Categorization of Transcriptome in the Species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) Exposed to Benzo[a]pyrene and Phenanthrene

Functional Categorization of Transcriptome in the Species Symphysodon aequifasciatus Pellegrin 1904 (Perciformes: Cichlidae) Exposed to Benzo[a]pyrene and Phenanthrene

Effects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar)

Modulation of telomerase activity in fish muscle by biological and environmental factors

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