Induction and cryopreservation of embryogenic cultures from nucelli and immature cotyledon cuts of mango (Mangifera indica L. var Zihua)

Wu, Yanan; Huang, Xue-Lin; Chen, Qizhu; Li, Xiaoju; Engelmann, Florent

doi:10.1007/s00299-006-0232-4

Cited by 25 publications

(7 citation statements)

References 15 publications

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“…However, we achieved the successful cryopreservation of P. omorika ET without prior coldhardening, and this reduced the costs and duration of preculture. A few reports on the effect of cryopreservation of the ET of coniferous tree species on their later potential for embryogenesis have shown that the proliferation potential of ET is the same as that of non-frozen control tissues (Kartha et al 1988;Lardet et al 2007;Wu et al 2007). However, we observed some differences in the growth rate of fresh ET on day 28 of culture, which amounted to 19.18 ± 2.0 and 16.00 ± 1.5 g in two control variants (Fig.…”

Section: Discussionmentioning

confidence: 97%

Cryopreservation of embryogenic tissues of Picea omorika (Serbian spruce)

Hazubska-Przybył

Chmielarz

Michalak

et al. 2010

Plant Cell Tiss Organ Cult

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Two-year-old embryogenic tissues (ET) of Picea omorika (Pančić) Purk. were successfully cryopreserved after preculture with sucrose, air-drying for 2 h, and freezing in liquid nitrogen (LN). The preculture protocol consisted of passaging the ET onto standard Litvay medium containing increasing concentrations of sucrose (0.25 M sucrose for 24 h, 0.5 M for 24 h, 0.75 M for 2 days, and 1.0 M for 3 days) for 7 days, at 25°C, and in the dark. The clumps were subsequently air-dried over silica gel, down to a 20% water content (based on fresh weight), placed in cryovials, and immersed in liquid nitrogen (LN) for 24 h. These were thawed at 42°C and progressively rehydrated in phytagel-solidified LM media containing decreasing concentrations of sucrose. After 3 weeks of in vitro culture, surviving clumps were friable and white in color, similar to their morphology prior to cryostorage. The frequency of bacterial and fungal contamination was higher if ET was frozen in LN-containing vials than in LN-free vials. This efficient cryopreservation protocol would be useful for the ex-situ conservation of P. omorika germplasm in gene banks at very low temperatures.

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Section: Discussionmentioning

confidence: 97%

Cryopreservation of embryogenic tissues of Picea omorika (Serbian spruce)

Hazubska-Przybył

Chmielarz

Michalak

et al. 2010

Plant Cell Tiss Organ Cult

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“…In this regard, somatic embryogenesis appears to be an efficient regeneration system. Somatic embryogenesis offers a number of applications as alternative pathways for mass multiplication of elite trees within a short time, germplasm conservation and genetic transformation (Wu et al 2007;GonzalezArnao et al 2008). The somatic embryogenesis process possesses immense potential for large-scale propagation with high commercial prospects.…”

Section: Introductionmentioning

confidence: 99%

Regeneration of pineapple (Ananas comosus L.) plant through somatic embryogenesis

Yapo

Kouakou

Koné

et al. 2011

J. Plant Biochem. Biotechnol.

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An efficient protocol for a complete plant regeneration by somatic embryogenesis was developed with Smooth Cayenne pineapple (Ananas comosus L.).Previous works showed that this species is responsive to somatic embryogenesis. In the present work the influence of components of culture medium in the induction, development and conversion of somatic embryos was investigate in order to establish a somatic embryogenesis protocol. Nodular callus (83.67%) was initiated from leaf explants of young plants on CIM 3 medium. The highest frequency (37.6%) of embryogenic callus induction was obtained from 4-week-old calluses on EIM 3 medium supplemented with 3.0 mg/l picloram. The highly organized callus induction and the development of somatic embryos were achieved after the transfer of callus clumps onto EIM 3 medium containing 1.0 mg/l BAP + 0.1 mg/l NAA. The frequency of somatic embryo formation was of 39.5± 2.45 embryos per callus. Up to 97% of the somatic embryos were converted into complete plants within 4 week on MSB medium with 1.0 mg/l BAP + 0.05 mg/l GA 3 + 500 mg/l glutamine. The continuation of the elongation of the shoots occurred on this medium). Shoots obtained from all the above methods were rooted in MSB medium with activated charcoal. Complete plantlets were transferred onto specially made polyethylene bags containing soil mixture and transferred to the greenhouse. Survival rate of the plantlets under ex vitro conditions was 98% and maximum average number of plantlets (80±0.6). The well-developed plantlets were transferred to an open field where the plants produced normal fruits.

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“…a biomass increase, a regeneration potential and production of secondary metabolites) is usually carried out using agar cultures in the post-thawing period (Bachiri et al 2000;Winkelmann et al 2004;Chen and Wang 2002;Zhang et al 2001;Suhartono et al 2005;Cho et al 2007;Wu et al 2007). a biomass increase, a regeneration potential and production of secondary metabolites) is usually carried out using agar cultures in the post-thawing period (Bachiri et al 2000;Winkelmann et al 2004;Chen and Wang 2002;Zhang et al 2001;Suhartono et al 2005;Cho et al 2007;Wu et al 2007).…”

Section: Insight Into the Recovery Of Cell Suspension Cultures After mentioning

confidence: 99%