Inducible plasmid-determined resistance to arsenate, arsenite, and antimony (III) in escherichia coli and Staphylococcus aureus

Silver, Simón; Budd, K.; Leahy, K M; Shaw, William V.; Hammond, David J.; Novick, Richard P.; Willsky, Gail R.; Malamy, Michael H.; Rosenberg, H.

doi:10.1128/jb.146.3.983-996.1981

Cited by 196 publications

(54 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Bacterial resistance to arsenic ions governed by plasmids was first discovered by Novick and Roth [71] in a group of Staphylococcus aureus /3-1actamase plasmids which also determine resistance to heavy metals. Arsenic resistance plasmids confer tolerance to both arsenate and arsenite as well as to antimony(IID [13,71,95]. Resistance to all three ions is inducible and cross-induction among them occurs [71,95].…”

Section: Plasmid-mediated Resistancementioning

confidence: 99%

“…Arsenic resistance plasmids confer tolerance to both arsenate and arsenite as well as to antimony(IID [13,71,95]. Resistance to all three ions is inducible and cross-induction among them occurs [71,95]. Arsenic resistance determinants are very common in Staphylococcal and Rhodococcus plasmids [34,71,88] but have also been found in plasmids of Gram-negative bacteria [13,15,39,65,66,79,101,102].…”

Section: Plasmid-mediated Resistancementioning

confidence: 99%

Section: Plasmid-mediated Resistancementioning

confidence: 99%

“…Silver et al [95] found that resistance to arsenate in both E. coli and S. aureus was due to reduced uptake of arsenate by resistant cells. As expected, high phosphate concentrations protected cells from arsenate toxicity [95]. Studies of resistant E. coli and S. aureus showed that the reduced arsenate uptake is due to an accelerated efflux of the toxic ions in a energy-dependent process [64,97].…”

Section: Plasmid-mediated Resistancementioning

confidence: 99%

See 3 more Smart Citations

Resistance to arsenic compounds in microorganisms

Cervantes

Ramı́rez

et al. 1994

FEMS Microbiology Reviews

285

143

View full text Add to dashboard Cite

Arsenic ions, frequently present as environmental pollutants, are very toxic for most microorganisms. Some microbial strains possess genetic determinants that confer resistance. In bacteria, these determinants are often found on plasmids, which has facilitated their study at the molecular level. Bacterial plasmids conferring arsenic resistance encode specific efflux pumps able to extrude arsenic from the cell cytoplasm thus lowering the intracellular concentration of the toxic ions. In Gram-negative bacteria, the efflux pump consists of a two-component ATPase complex. ArsA is the ATPase subunit and is associated with an integral membrane subunit, ArsB. Arsenate is enzymatically reduced to arsenite (the substrate of ArsB and the activator of ArsA) by the small cytoplasmic ArsC polypeptide. In Gram-positive bacteria, comparable arsB and arsC genes (and proteins) are found, but arsA is missing. In addition to the wide spread plasmid arsenic resistance determinant, a few bacteria confer resistance to arsenite with a separate determinant for enzymatic oxidation of more-toxic arsenite to less-toxic arsenate. In contrast to the detailed information on the mechanisms of arsenic resistance in bacteria, little work has been reported on this subject in algae and fungi.

show abstract

Section: Plasmid-mediated Resistancementioning

confidence: 99%

Section: Plasmid-mediated Resistancementioning

confidence: 99%

Section: Plasmid-mediated Resistancementioning

confidence: 99%

Section: Plasmid-mediated Resistancementioning

confidence: 99%

See 2 more Smart Citations

Resistance to arsenic compounds in microorganisms

Cervantes

Ramı́rez

et al. 1994

FEMS Microbiology Reviews

285

143

View full text Add to dashboard Cite

show abstract

“…In nosocomial bacteria, in addition to the expected genes conferring antibiotic resistance and selected by the use of these agents in therapeutic procedures, genes for resistance to heavy metals may also be present. Thus, bacteria isolated from hospital infections have been found to contain genes that confer resistance to inorganic ions derived from mercury (Porter et al, 1982;Masaru et al, 2004), cadmium (Nucifora et al, 1989), silver (Gupta et al, 2001), and arsenic (Silver et al, 1981), among others. These bacteria possess heavy-metal-resistance genes that are present on chromosomes, plasmids, or transposons (reviewed in Silver & Phung, 2005).…”

Section: Introductionmentioning

confidence: 99%

Chromate-resistance genes in plasmids from antibiotic-resistant nosocomial enterobacterial isolates

Caballero-Flores¹,

Acosta-Navarrete²,

Ramírez-Díaz³

et al. 2011

FEMS Microbiol Lett

View full text Add to dashboard Cite

The presence of chromate-resistance genes in enterobacteria was evaluated in a collection of 109 antibiotic-resistant nosocomial isolates from nine major cities in México. Results were compared with the presence of mercury-resistance genes. Susceptibility tests showed that 21% of the isolates were resistant to chromate (Cr(R)), whereas 36% were resistant to mercury (Hg(R)). Cr(R) levels were high in Klebsiella pneumoniae (61%), low in Enterobacter cloacae (12%) and Escherichia coli (4%), and null in Salmonella sp. isolates. Colony hybridization demonstrated that the majority of metal-resistant isolates hybridized with chrA gene (87% of Cr(R) isolates), encoding a CHR transporter homologue, and merA gene (74% of Hg(R) isolates), encoding MerA mercuric reductase, suggesting that most isolates expressed these widespread metal-resistance systems. Southern blot hybridization of Cr(R) isolates showed that plasmids of 80, 85, and 95 kb from K. pneumoniae isolates, and of 100 kb from an E. cloacae isolate, contained chrA-related sequences. These plasmids belonged to IncN or IncP incompatibility groups, and conferred Cr(R), as well as multiple antibiotic resistance, when transferred by conjugation to an E. coli standard strain. These data indicated that Cr(R) genes may be distributed among clinical enterobacteria via conjugative plasmids, probably by coselection with antibiotic-resistant genes.

show abstract

Isolation of Three Contiguous Genes,ACR1,ACR2 andACR3, Involved in Resistance to Arsenic Compounds in the YeastSaccharomyces cerevisiae

Bobrowicz

Wysocki

Owsianik

et al. 1997

Yeast

214

166

View full text Add to dashboard Cite

A 4·2 kb region from Saccharomyces cerevisiae chromosome XVI was isolated as a yeast fragment conferring resistance to 7 mM‐sodium arsenite (NaAsO2), when put on a multicopy plasmid. Homology searches revealed a cluster of three new open reading frames named ACR1, ACR2 and ACR3. The hypothetical product of the ACR1 gene is similar to the transcriptional regulatory proteins, encoded by YAP1, and YAP2 genes from S. cerevisiae. Disruption of the ACR1 gene conduces to an arsenite and arsenate hypersensitivity phenotype. The ACR2 gene is indispensable for arsenate but not for arsenite resistance. The hypothetical product of the ACR3 gene shows high similarity to the hypothetical membrane protein encoded by Bacillus subtilis ORF1 of the skin element and weak similarity to the ArsB membrane protein of the Staphylococcus aureus arsenical‐resistance operon. Overexpression of the ACR3 gene confers an arsenite‐ but not an arsenate‐resistance phenotype. The presence of ACR3 together with ACR2 on a multicopy plasmid expands the resistance phenotype into arsenate. These findings suggest that all three novel genes: ACR1, ACR2 and ACR3 are involved in the arsenical‐resistance phenomenon in S. cerevisiae. © 1997 John Wiley & Sons, Ltd.

show abstract

Inducible plasmid-determined resistance to arsenate, arsenite, and antimony (III) in escherichia coli and Staphylococcus aureus

Cited by 196 publications

References 30 publications

Resistance to arsenic compounds in microorganisms

Resistance to arsenic compounds in microorganisms

Chromate-resistance genes in plasmids from antibiotic-resistant nosocomial enterobacterial isolates

Isolation of Three Contiguous Genes,ACR1,ACR2 andACR3, Involved in Resistance to Arsenic Compounds in the YeastSaccharomyces cerevisiae

Contact Info

Product

Resources

About