Induced breeding, embryonic and larval development of Koi carp (Cyprinus carpio) in Khulna, Bangladesh

Abstract

The aim of the present study was to perform induced breeding and to observe embryonic and larval development stages in different seasons for mature male and female Koi carp, Cyprinus carpio, was done by administering the synthetic hormone ovaprim, in a single dose for males and a double dose for females at 0.4-1.0 ml/kg body weight via an intramuscular injection. Spawning occurred between 5-6 hrs after the 2nd injection in summer (April) at a temperature of 26-29 oC and 17-20 0C in winter (December). Using doses of 0.4ml/kg, 0.7ml/kg and 1.0 ml/kg of ovaprim, the fertilization rate of eggs were obtained at 42.31%, 54.55% and 61.82 % in summer and 26.92%, 38.00% and 39.23 % in winter. Hatching rates were 42.78%, 44.60% and 55.00 % in the summer season and 30.22%, 31.01% and 31.67 % in the winter season with the same doses of ovaprim. The fertilized eggs were adhesive, transparent, and spherical with diameters ranging between 0.8 mm and 1.0 mm and yellowish white in color. Due to a positive response to the hormone Ovaprim, considerable fertilization and hatching rate, short embryonic period and larval development, it is possible to conduct a successful breeding program of this species commercially

Short-Term Storage of Japanese Koi (Cyprinus carpio var. koi) Sperm on the Egg Fertilization Performance

“…The results of the hatching rates of the fertilized eggs from the short-term stored sperm (47.5 ± 3.7%) were also not significantly different (P>0.05) from those of the freshly collected sperm (48.3 ± 7.3%). The fertilization and hatching rates obtained in this study are very similar to those obtained in the study by Ghosh et al (2012), which was conducted on koi carp during the summer season. However, compared to the study of Md.…”

“…All differences between treatment groups and control were considered significantly different at P<0.05 using Tukey's test (ANOVA) for pairwise comparison for the short-term storage experiment and the two-sample T-test for the egg fertilization experiment. Sperm density (equation 1), total number of eggs produced (fecundity) (equation 2), percentages of egg fertilized (equation 3), and hatching (equation 4) were calculated using the following formulas (WHO, 2010;Ghosh et al 2012;Okomoda et al, 2017;Viader-Guerrero et al, 2021):…”

“…This is likely because the broodfish in the current study are very young. In a study by Ghosh et al (2012) on the same species, fish fecundity was proportional to fish size, with larger broodfish producing a greater number of eggs. The relative fecundity of the three female broodfish in the present study ranged from 33,756 eggs per kg to 171,640 eggs per kg, with a mean relative fecundity of 89,767 eggs per kg (Table 2).…”

“…. Previously, C. carpio had naturally reproduced in the pond without much human intervention (Ghosh et al, 2012;Md. Monirul et al, 2016).…”

“…Therefore, we practice induced breeding of Japanese koi to facilitate control and management, as our NBC can only breed and raise fish in indoor tanks. The induced breeding technique has long been used in aquaculture to increase the production of fish larvae and fry, and this approach is widely used in carp fish production including Japanese koi (Ghosh et al, 2012;Md. Monirul et al, 2016;Gouda et al, 2020).…”