Induced Accumulation and Potential Antioxidative Function of Rutin in Two Cultivars of Nicotiana tabacum L.

Abstract

The rutin (quercetin-3-rhamnosyl-glucoside) content of two tobacco cultivars (Nicotiana tabacum L.) which differ in their ozone-sensitivity was assayed after exposure to various rutininducing stimuli. In the growth-chamber, UV radiation in combination with white light led to the accumulation of similar amounts of rutin in both cultivars. Treatment with radical producing agents (tert-butylhydroperoxide and paraquat) also led to rutin accumulation. In this case, the rutin content was higher in the tolerant cultivar. The rutin content was also higher in the tolerant cultivar upon exposure of the plants on an out-door stand, even when the UV-part of the sun spectrum was excluded by cut-off filters. The potential role of rutin as antioxidant was tested with an ion leakage assay. Plants with relatively high rutin content were less sensitive towards paraquat-induced ion leakage than plants without rutin. Thus, the higher rutin content of the ozone-tolerant cultivar Bel B may well contribute to its tolerance against oxidative stress.

Increasing Catechin and Procyanindin Accumulation in High-CO2-Treated Fragaria vesca Strawberries

“…27−30 It has been reported that flavonol glucosides of quercetin and kaempferol have multiple functional roles in plant responses to abiotic stress, 31 including water deficit stress in response to UV-B radiation, and may contribute to ozone tolerance. 32,33 Moreover, studies with strawberries grown under UV-blocking film exhibited lower contenst of quercetin 3glucuronide and kaempferol 3-glucoside than those grown in the open field. 34 In accordance with our results, the increase in flavonols in response to 20% CO 2 treatment may reflect a CO 2 tolerance strategy, and so no such accumulation was observed in 40% CO 2 -treated fruit.…”

“…On the contrary, no increase in kaempferol 3-glucuronide and quercetin 3-glucoronide levels occurred in fruit treated with 40% CO 2 , although these levels did not differ from those found at time of harvest. It is well-known that the amounts of flavonols vary substantially among the different strawberry cultivars, although, in general, quercetin and kaempferol are the major flavonols and occur as 3-glucosides and 3-glucuronides. − It has been reported that flavonol glucosides of quercetin and kaempferol have multiple functional roles in plant responses to abiotic stress, including water deficit stress in response to UV-B radiation, and may contribute to ozone tolerance. , Moreover, studies with strawberries grown under UV-blocking film exhibited lower contenst of quercetin 3-glucuronide and kaempferol 3-glucoside than those grown in the open field . In accordance with our results, the increase in flavonols in response to 20% CO 2 treatment may reflect a CO 2 tolerance strategy, and so no such accumulation was observed in 40% CO 2 -treated fruit.…”

Glucosylation of the phytoalexin N‐feruloyl tyramine modulates the levels of pathogen‐responsive metabolites in Nicotiana benthamiana

“…A highly active rhamnosyltransferase must be present to transfer rhamnose onto the flavonoid glucosides to produce the rutinosides. The production of rutin (quercetin rutinoside) by N. tabacum has been shown to be affected by light quality (Fu et al, 2016), radical producing agents (Steger-Hartmann et al, 1994) and the transcription factor gene NtFLS2 (Shi et al, 2017). However, the level of rutin was unchanged after methyl jasmonate treatment of N. attenuata (Kein€ anen et al, 2001).…”