Induce Hyperlipidemia in Rats Using High Fat Diet Investigating Blood Lipid and Histopathology

Karam, Isam; Ma, Ning; Yang, Ye; Jy, Li

doi:10.15744/2455-7641.4.104

Cited by 10 publications

(3 citation statements)

References 12 publications

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“…As a reported example, a study on Wistar rats established that several weeks into a regimen of HFD lead to obesity and dyslipidemia (Rosnah et al, 2022). Another study (Karam, Ma, Yang, & Li, 2018) on the same species of rats as in the current study (Sprague-Dawley rats) has also reported significant increase in hyperlipidemia biomarkers after only 7 weeks of consumption of HFD with the following macronutrients (41.5% lipids, 40.2% carbohydrates, and 18.3% proteins (kcal)).…”

Section: Discussionsupporting

confidence: 54%

Serum VCAM-1 reduction by phytosomal curcumin formulation in rats on a high-fat diet

Abdul Razzak,

Al Kafaji,

Khan

et al. 2023

AGJSR

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PurposeThis paper aims to evaluate the effect of consumption of a high-fat diet (HFD) rich with total saturated fats on adiposity and serum levels of vascular cell adhesion molecule (sVCAM-1), a biomarker of endothelial inflammation/dysfunction. Another aim is to evaluate whether supplementation of a phytosomal formulation of curcumin would reduce adiposity measures and sVCAM-1 levels in HFD rats.Design/methodology/approachThe study was conducted on 17 male rats which were allocated to one of three feeding regimen groups: normal diet (ND); HFD, or HFD with dietary phytosomal curcumin (HFD-C). Anthropometric measures were recorded weekly up to 20 weeks of feeding intervention, at the end of which, sVCAM-1 levels were also compared with one-way ANOVA and Tukey post-hoc analysis.FindingsThe HFD group had the greatest values for raw anthropometric data, and there was a group difference in anthropometric measures, however there was no significant difference between HFD and HFD-C for any measure. The gain at 20 weeks from initial values did reveal significant differences in weight and abdominal circumference between HFD and HFD-C groups. There were significant group differences in sVCAM-1 levels, with only HFD-C displaying significant lower levels than HFD group.Originality/valueThis is the first study that shows the capacity of a phytosomal formulation of curcumin in reducing adiposity and sVCAM-1 levels during daily intake of saturated fats above the recommended level. The results are promising in that this formulation can protect against endothelial inflammation/dysfunction, and can be used as complimentary therapy to suppress dyslipidemia/obesity-related cardiovascular complications.

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Section: Discussionsupporting

confidence: 54%

Serum VCAM-1 reduction by phytosomal curcumin formulation in rats on a high-fat diet

Abdul Razzak,

Al Kafaji,

Khan

et al. 2023

AGJSR

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“…According to sampling and trimming procedures, the pancreatic tissue was dehydrated and paraffinized [26]. In an automatic procedure, a section of about 4µm was stained by conventional hematoxylin and eosin (H&E) stains [27].…”

Section: Histological Examinationmentioning

confidence: 99%

Potential hepato-protective effect of Salvia hispanica (chia) and Chenopodium quinoa (quinoa) in diabetic male albino rats

Eshraa,

El Sahn,

Aborhyem

2024

IJPHS

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Chia and quinoa seeds are novel sources of bioactive compounds that may improve lipid profile, blood glucose homeostasis, and body weight in diabetic rats. This work aims to study the effect of different percentages of chia and quinoa consumption, alone or in a mixture, compared to Metformin drug on regulating lipid profile, blood glucose level, and weight of male diabetic rats. The consumption of quinoa and chia seeds showed a significant decline in fasting blood glucose (FBG), fasting insulin level, Hemoglobin A1C (HbA1c), and Homeostatic Model Assessment for Insulin (HOMA-IR), while the best results were obtained with Metformin and chia seeds. A decrease in mean body weight, triglycerides, total cholesterol, low density lipoprotein (LDL-C), and very low-density lipoprotein (VLDL-C), and an increase in high density lipoprotein (HDL-C) occurred, which was significant when consuming chia and quinoa seeds rather than treatment with metformin. Chia and quinoa seeds could improve blood glucose homeostasis and lipid profile in diabetic rats. These research findings could promote the daily consumption of quinoa and chia as great functional foods that could improve blood glucose homeostasis and lipid profile.

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“…A total of forty-five rats were randomly divided into nine groups of five each. Hyperlipidemia was induced in rats by feeding them with high-fat diet as done by Karam et al [23] with modification in the feeding duration (35 days). After the 35 days feeding exercise, blood sample were collected from their tails after immersed in water bath at 45 o C for 5 min to make the blood vessel swell in order to get the desire volume (1-1.5 ml).…”

Section: Experimental Designmentioning

confidence: 99%

Antihyperlidemic Efficiency of Abelmoschus esculentus (Okra) Fruits Varieties on Rats Fed High-Fat Diet

Nuhu

Mhya

Atiku

et al. 2020

JALSI

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Background: Okra fruits have been found to be efficient in managing hyperlipidemia. But, there are different varieties of Okra, and whether antihyperlipidemic efficiency varies with the varieties has not been reported. Aim of the Study: The aim of the study was to validate antihyperlipidemic efficiencies of some Okra fruits varieties on rats fed high-fat diet. Methods: The five varieties of okra fruit was each sliced, air dried and pulverized into powder then extracted with methanol (80%) using Soxhlet extractor and concentrated at 30∘C in a rotary evaporator then finally air dried. Qualitative and quantitative phytochemicals, and proximate analysis were conducted on the extracts. Hyperlipidemia was induce by feeding rats with high-fat diet for 35 days, followed by treating with two selected Okra fruit varieties (NHB-AI-B and Yar kolon) which has the highest extract yield and phytochemicals for 21 days. Nine groups of five rats was used: groups 1-3 (hyperlipidemic rats received NHB-AI-B Okra fruit extract at different doses), group 4-6 (hyperlipidemic rats received Yar kolon Okra fruit extract at different doses), group 7 (positive control rats treated with 10mg/kg Atorvastatin), group 8 (normal control rats fed basal diet) and group 9 (negative control). Serum lipid profile were determined from each rat. Results: The study confirmed the presence of some important phytochemicals like saponins, tannins, phenolics, flavonoids, fibre etc but differs in concentrations among the varieties where Yar kolon variety emerge highest in almost all the chemical compounds. Rat fed high-fat diet for 35 days developed hyperlipidemia as evident by the elevated triglyceride, cholesterol, LDL-C, body weight and supressed HDL-C. When treated with extracts of NHB-AI-B and Yar kolon Okra fruits varieties, the altered lipid profile were significantly reversed toward normalcy with Yar kolon variety exerting the most efficient activity. Conclusion: The study showed the five Okra fruit varieties possess same chemical compounds but differs in concentrations among varieties. The extracts of Okra fruits varieties exert significant antihyperlipidemic effect but in varied degrees suggesting variations in their efficiency. This, therefore calls for further study to compare more Okra varieties to determine which one is the most potent and its active agent.

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Induce Hyperlipidemia in Rats Using High Fat Diet Investigating Blood Lipid and Histopathology

Cited by 10 publications

References 12 publications

Serum VCAM-1 reduction by phytosomal curcumin formulation in rats on a high-fat diet

Serum VCAM-1 reduction by phytosomal curcumin formulation in rats on a high-fat diet

Potential hepato-protective effect of Salvia hispanica (chia) and Chenopodium quinoa (quinoa) in diabetic male albino rats

Antihyperlidemic Efficiency of Abelmoschus esculentus (Okra) Fruits Varieties on Rats Fed High-Fat Diet

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