Indole-3-Acetic Acid (IAA) Production in Symbiotic and Non-Symbiotic Nitrogen-Fixing Bacteria and its Optimization by Taguchi Design

Shokri, Dariush; Emtiazi, Giti

doi:10.1007/s00284-010-9600-y

Cited by 92 publications

(65 citation statements)

References 14 publications

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“…To assess indole-3-acetic acid (IAA) production, the method described by Glickmann and Dessaux (1995) was used. Some modifications for in vitro IAA production were performed by using modified King B broth (Casein 10 g/l, Peptone no.3 10 g/l, dipotassium phosphate 1.5 g/l, magnesium sulfate 1.5 g/l) media with and without additional L-tryptophan (3 mg/ml), as described by Shokri and Emtiazi (2010). The production of IAA was measured after 6 days of incubation.…”

Section: Chemotaxonomic Characterizationmentioning

confidence: 99%

Paenibacillus yonginensis sp. nov., a potential plant growth promoting bacterium isolated from humus soil of Yongin forest

Sukweenadhi

Kim

Lee

et al. 2014

Antonie van Leeuwenhoek

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Strain DCY84 T , a Gram-stain positive, rodshaped, aerobic, spore-forming bacterium, motile by means of peritrichous flagella, was isolated from humus soil from Yongin forest in Gyeonggi province, South Korea. Strain DCY84 T shared the highest sequence similarity with Paenibacillus barengoltzii KACC 15270 T (96.86 %), followed by Paenibacillus timonensis KACC 11491 T (96.49 %) and Paenibacillus phoenicis NBRC 106274 T (95.77 %). Strain DCY84 T was found to able to grow best in TSA at temperature 30°C, at pH 8 and at 0.5 % NaCl. MK-7 menaquinone was identified as the isoprenoid quinone. The major polar lipids were identified as phosphatidylethanolamine, an unidentified aminophospholipid, two unidentified aminolipids and an unidentified polar lipid. The peptidoglycan was found to contain the amino acids meso-diaminopimelic acid, alanine and D-glutamic acid. The major fatty acids of strain DCY84 T were identified as branched chain anteiso-C 15:0 , saturated C 16:0 and branched chain anteiso-C 17:0 . The cell wall sugars of strain DCY84 T were found to comprise of ribose, galactose and xylose. The major polyamine was identified as spermidine. The DNA G?C content was determined to be 62.6 mol%. After 6 days of incubation, strain DCY84 T produced 52.96 ± 1.85 and 72.83 ± 2.86 lg/ml L-indole-3-acetic acid, using media without L-tryptophan and supplemented with Ltryptophan, respectively. Strain DCY84 T was also found to be able to solubilize phosphate and produce siderophores. On the basis of the phenotypic characteristics, genotypic analysis and chemotaxonomic characteristics, strain DCY84 T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus yonginensis sp. nov. is proposed. The type strain is DCY84 T (=KCTC 33428 T = JCM 19885 T ).

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Section: Chemotaxonomic Characterizationmentioning

confidence: 99%

Paenibacillus yonginensis sp. nov., a potential plant growth promoting bacterium isolated from humus soil of Yongin forest

Sukweenadhi

Kim

Lee

et al. 2014

Antonie van Leeuwenhoek

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“…In addition, carbon and nitrogen sources have been proven as essential factors influencing bacterial and fungal IAA production. [32][33][34][35] In our investigation of IAA production by yeast isolates at different temperatures, the optimal temperature for IAA production in the majority of the isolates was 28 C, instead of 37 C and 16 C. In addition, in earlier studies, fungal IAA production was maximal at 28 C. 36,37 However, in our study, of the 12 tested yeast isolates, 3 and 2 isolates produced higher levels of IAA at 37 C and 16 C, respectively, than that at 28 C. These studies proved that bacteria and yeasts could be excellent models for studying the physiological and biochemical mechanisms of IAA production. More studies may further provide opportunities in environmentally sustainable approach to increase crop production.…”

Section: Iaa Biosynthetic Pathwaysmentioning

confidence: 99%

Indole-3-acetic acid: A widespread physiological code in interactions of fungi with other organisms

Wei

Chen

et al. 2015

Plant Signaling & Behavior

251

142

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“…The IAA production by the B. cereus isolates started after the inoculation and maximum IAA production was obtained in the stationary growth phase at 72h followed by a decrease. As stated previously, the decrease in IAA production after 72 h might be due to the synthesis of IAA degrading enzymes such as IAA oxidase and peroxidase [17,18]. Unyayar et al [19] reported that the amount of these enzymes were high in the stationary phase.…”

Section: Screening Of B Cereus Isolates For Iaa Productionmentioning

confidence: 97%

Biosynthesis Of Indole-3-Acetic Acid By Bacillus cereus Immobilized Cells

Özdal¹,

Ozdal²,

Sezen³

et al. 2016

CSJ

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Abstract. Four different strains of Bacillus cereus producing indole-3-acetic acid (IAA) were isolated from various rhizospheric soils and characterized biochemically. The isolates were screened for (IAA) production and BC-On strain was found to be the best IAA producer with 46.25 mg/l. Maximum IAA production was obtained in the stationary growth phase at 72h. Significant correlation was also observed between IAA production and growth of the B. cereus strains. Among the isolates, BC-On strain was further used for immobilization studies. Maximum IAA production was obtained at initial pH of 8.0 and temperature of 35 °C after 18 h of fermentation. The immobilized cells could be effectively reused thirteen times and the IAA concentration of 300 mg/l was determined during this period. Results showed that immobilized cells can be used in the continuous process for the production of IAA. The productivity obtained by immobilization was higher than the one obtained by submerged cultivation and immobilization reduced the fermentation time.Keywords: Indole-3-acetic acid, Rhizobacteria, Bacillus cereus, Immobilization Immobilize Bacillus cereus Hücreleri Tarafından İndol-3-Asetik Asitin BiyosenteziÖzet. İndol-3-asetik asit (İAA) üreten dört farklı Bacillus cereus straini çeşitli rizosfer topraklarından izole edildi ve biyokimyasal olarak karakterize edilmiştir. İzolatlar İAA üretimi için taranmış ve BC-On straini 46.25 mg/l ile en iyi İAA üreticisi olarak bulunmuştur. En fazla IAA üretimi 72. saatte durağan büyüme fazında elde edilmiştir. B. cereus strainlerinin IAA üretimi ve büyüme arasında önemli bir ilişki gözlenmiştir. İzolatlar arasında, BC-On straini immobilizasyon çalışmalarında kullanılmıştır. En fazla IAA üretimi 18 saatlik fermentasyon sonrasında başlangıç pH'sı 8.0 ve 35 °C elde edilmiştir. İmmobilize edilmiş hücreler etkili olarak on üç kez kullanılabilmiş ve bu süre boyunca 300 mg/l İAA konsantrasyonu tespit edilmiştir. Sonuçlar immobilize hücrelerin IAA üretimi için sürekli bir işlemde kullanılabilir olduğunu göstermiştir. İmmobilizasyon ile elde edilen verimlilik, derin fermentasyon ile elde edilenden daha yüksek olduğu bulunmuş ve immobilizasyon fermentasyon süresini kısaltmıştır.

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Indole-3-Acetic Acid (IAA) Production in Symbiotic and Non-Symbiotic Nitrogen-Fixing Bacteria and its Optimization by Taguchi Design

Cited by 92 publications

References 14 publications

Paenibacillus yonginensis sp. nov., a potential plant growth promoting bacterium isolated from humus soil of Yongin forest

Paenibacillus yonginensis sp. nov., a potential plant growth promoting bacterium isolated from humus soil of Yongin forest

Indole-3-acetic acid: A widespread physiological code in interactions of fungi with other organisms

Biosynthesis Of Indole-3-Acetic Acid By Bacillus cereus Immobilized Cells

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