Individual redox characteristics and kinetic properties of the hemes in cytochromes c3: New methods of investigation

Control of the redox potential in c-type cytochromes: importance of the entropic contribution

“…It follows that, for this heme, the temperature dependence of the microscopic potential is directly obtained from redox titrations monitored by absorbance variations. The same comment applies to some extent to the highest potential heme of D. vulgaris Miyazaki cytochrome c3 (Bertrand et al, 1994).…”

“…the interaction potentials determined by EPR and NMR (Bertrand et al, 1994). Although these interaction potentials differ significantly, they lead to rather similar thermodynamic parameters (Table 3).…”

Control of the redox potential in c-type cytochromes: importance of the entropic contribution

“…It follows that, for this heme, the temperature dependence of the microscopic potential is directly obtained from redox titrations monitored by absorbance variations. The same comment applies to some extent to the highest potential heme of D. vulgaris Miyazaki cytochrome c3 (Bertrand et al, 1994).…”

“…the interaction potentials determined by EPR and NMR (Bertrand et al, 1994). Although these interaction potentials differ significantly, they lead to rather similar thermodynamic parameters (Table 3).…”

Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements

“…This would be wrong in the case of redox enzymes where the electron transfer steps are reversible. The present work therefore adds to the very few studies where the reversibility of the electron transfer steps has been considered. ,,, …”

A Comparative Multi-Frequency EPR Study of Dipolar Interaction in Tetra-Heme Cytochromes

“…Non-colinearity can give rise to asymmetries in the spectral features [33], which we have ignored here to keep the simulation problem tractable. Second, we have limited the set of g xyz values to approximately obey Equation (2), which is essentially based on the assumption of ionic coordination [34]. Third, since the g values are distributed due to a distribution of protein conformations, the mutual Fe-Fe dipolar interactions may also be well distributed, leading to distributions in Fe-Fe distances and in the Euler angles between the g matrices, which we have also ignored for tractability reasons.…”

Control of the redox potential in c-type cytochromes: importance of the entropic contribution

“…It follows that, for this heme, the temperature dependence of the microscopic potential is directly obtained from redox titrations monitored by absorbance variations. The same comment applies to some extent to the highest potential heme of D. vulgaris Miyazaki cytochrome c3 (Bertrand et al, 1994).…”

“…the interaction potentials determined by EPR and NMR (Bertrand et al, 1994). Although these interaction potentials differ significantly, they lead to rather similar thermodynamic parameters (Table 3).…”

Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements

“…This would be wrong in the case of redox enzymes where the electron transfer steps are reversible. The present work therefore adds to the very few studies where the reversibility of the electron transfer steps has been considered. ,,, …”

A Comparative Multi-Frequency EPR Study of Dipolar Interaction in Tetra-Heme Cytochromes

“…Non-colinearity can give rise to asymmetries in the spectral features [33], which we have ignored here to keep the simulation problem tractable. Second, we have limited the set of g xyz values to approximately obey Equation (2), which is essentially based on the assumption of ionic coordination [34]. Third, since the g values are distributed due to a distribution of protein conformations, the mutual Fe-Fe dipolar interactions may also be well distributed, leading to distributions in Fe-Fe distances and in the Euler angles between the g matrices, which we have also ignored for tractability reasons.…”

Control of the redox potential in c-type cytochromes: importance of the entropic contribution

“…It follows that, for this heme, the temperature dependence of the microscopic potential is directly obtained from redox titrations monitored by absorbance variations. The same comment applies to some extent to the highest potential heme of D. vulgaris Miyazaki cytochrome c3 (Bertrand et al, 1994).…”

“…the interaction potentials determined by EPR and NMR (Bertrand et al, 1994). Although these interaction potentials differ significantly, they lead to rather similar thermodynamic parameters (Table 3).…”

Rates of Intra- and Intermolecular Electron Transfers in Hydrogenase Deduced from Steady-State Activity Measurements

“…This would be wrong in the case of redox enzymes where the electron transfer steps are reversible. The present work therefore adds to the very few studies where the reversibility of the electron transfer steps has been considered. ,,, …”

A Comparative Multi-Frequency EPR Study of Dipolar Interaction in Tetra-Heme Cytochromes

“…Non-colinearity can give rise to asymmetries in the spectral features [33], which we have ignored here to keep the simulation problem tractable. Second, we have limited the set of g xyz values to approximately obey Equation (2), which is essentially based on the assumption of ionic coordination [34]. Third, since the g values are distributed due to a distribution of protein conformations, the mutual Fe-Fe dipolar interactions may also be well distributed, leading to distributions in Fe-Fe distances and in the Euler angles between the g matrices, which we have also ignored for tractability reasons.…”