Indirect Spectrophotometric Determination of Thiamine Hydrochloride in Presence of Sulphite Via Chromium-1,5-Diphenylcarbazide Complex

Shekho, Najih H.; Al-Hadi, Bassima A. Abed; Sarsam, Lamya A.

doi:10.33899/rjs.2013.77817

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…The bleaching method was applied for determination of Sulphite in various water samples, so that (1-7) mL were added to volumetric flasks containing different concentrations of Sulphite (5-100) µg/25ml , then measured the absorption at the chosen wavelength as shown in Table 5. [33] for 5 degrees of freedom at the confidence level (95%) did not exceed theoretical values when the present method was compared with the literature method [34]as it was tabulated in Table 6.…”

Section: Application Of the Present Methodmentioning

confidence: 86%

Estimation of thiamine hydrochloride and sulphite using charge transfer complex reaction

Saleem¹

2021

sjpas

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A new spectrophotometric method is used for estimating Thiamine hydrochloride in its pure form and pharmaceutical formulations, the present method depends on the reaction between Thiamine hydrochloride and chrome azurol S to form a red complex which exhibit maximum absorption at 510 nm, the addition of Sulphite to the resulted red complex is bleaching it and this step was used for the determination of Sulphite which is considered an attacker of Thiamine hydrochloride, therefore, this method was developed for determination of Thiamine hydrochloride in presence of Sulphite. The linearity of the proposed method was obeyed Beer’s law from 2 to 48 and 0.04 to 2.4 ppm for Thiamine hydrochloride and Sulphite respectively. Also, the method sensitivity was measured by molar absorptivity values 5.9×104 l.mol-1.cm-1 and 1.68×104 l.mol-1.cm-1 for Thiamine hydrochloride and Sulphite respectively. The present method accuracy (average recovery) 100.06% and 100.04% the precision (RSD) of the method is ±0.98 - ±1.27% and ±0.76 - ±1.47 of Thiamine hydrochloride and Sulphite respectively. This method was applied for the estimation of B1 and SO32- in pharmaceutical preparations and various water samples respectively.

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Section: Application Of the Present Methodmentioning

confidence: 86%

Estimation of thiamine hydrochloride and sulphite using charge transfer complex reaction

Saleem¹

2021

sjpas

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“…A literature review showed that very few analytical methods have been reported for optimization of spectroscopy conditions in UV-visible Spectrophotometry methods using two-level full factorial design [8] Central Composite Face Centered Design [9] Box-Behnken design [10]. A few UV-visible Spectrophotometry methods have been formerly reported for the estimation of Thiamine Hydrochloride in pharmaceuticals [11][12][13][14]. Other techniques reported for the assay of Thiamine Hydrochloride in pharmaceuticals include Fluorimetry [15,16], HPLC [17][18][19][20][21][22], Polarography method [23,24] and Voltammetry [25].…”

Section: Introductionmentioning

confidence: 99%

Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation

Sharma¹,

Hp²,

Aryal³

et al. 2021

Preprint

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UV-Visible spectroscopy method has been developed for the analysis of Thiamine in biological and pharmaceutical product, based on a chromogenic derivatizing reaction using PDAB (P- dimethyl amino benzaldehyde) reagent. A Central Composite Design (CCD) design with response surface methodology was executed for optimization of experimental conditions of drug with a smaller number of experimental trials. Three independent factors, the concentration of PDAB, the volume of PDAB reagent, and volume of HCl, were used to construct a mathematical model and study the effects of these independent factors on responses as absorbance. The colored complex exhibits a Red shift with absorption maximum λmax at 425 nm, which was selected as the analytical wavelength. The drug seems to be linear, which was established via the regression analysis from 5-30 μgmL-1, with an R2 value of 0.998.The % RSD for intraday and interday precision was < 2%, with good recovery ranging from 95.02 to 101.43% with biological and pharmaceutical samples. LOD and LOQ of the developed method were found to be 1.51 μg mL-1 and 4.57 μg mL-1.This method can be used in routine analysis of pharmaceutical products containing aromatic primary amines along with an estimation of biological samples like urine, blood, sweat, faeces.

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Spectrophotometric Determination of Mefenamic acid in pure and Pharmaceutical Preparations

Saleem

Alnuaimy

2021

J. Phys.: Conf. Ser.

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Chromate ions CrO4 −2 have been used in three simples, rapid, an accurate spectrophotometric method. These methods were suggested to estimate mefenamic acid in its pure form and pharmaceutical preparations. These methods were different from each other with reactants components, and they are similar as, indirect methods and depends on oxidation – reduction reaction. So that, Method 1, based on oxidation reduction reaction between mefenamic acid and chromate ion in presence of acidic medium and sodium oxalate to form a water soluble, and stable pinkish-violet colored complex with 1,5-diphenylcarbazide as a reagent which was measured at λmax 545 nm. Method 2 depends on oxidizing mefenamic acid with chromate ions in presence of sodium oxalate as a catalyst agent and acidic medium to form with indigo carmine λmax at 610 nm. water soluble and stable blue complex. Finally, method 3 based on the reaction between mefenamic acid and chromate ion in presence of sodium oxalate and acidic medium to form with chrome azurol S a red complex measured λmax at 512 nm. These three methods have been followed Beer’s law at (2-40,1.2-40 and 2-36) μg/mL methods 1,2 and 3 respectively, while molar absorptivity and Sandall’s sensitivity 0.84×104, 0.54×104 and 1.08×104 1.mol−1.cm−1 and 0.0287, 0.0446 and 0.0223 μg.cm−2 for methods 1,2 and 3 respectively. The suggested methods were applied successfully to determination of mefenamic acid in pharmaceutical formulation with good recoveries.

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Indirect Spectrophotometric Determination of Thiamine Hydrochloride in Presence of Sulphite Via Chromium-1,5-Diphenylcarbazide Complex

Cited by 4 publications

References 23 publications

Estimation of thiamine hydrochloride and sulphite using charge transfer complex reaction

Estimation of thiamine hydrochloride and sulphite using charge transfer complex reaction

Design of Experimental Approach Biosensor for the Development and Validation of UV Spectrophotometry Method for Determination of Thiamine in Biological Samples and Tablet Formulation

Spectrophotometric Determination of Mefenamic acid in pure and Pharmaceutical Preparations

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