Indications for chlororespiration in relation to light regime in the marine diatom Thalassiosira weissflogii

Dijkman, Nicole A.; Kroon, Bernd M. A.

doi:10.1016/s1011-1344(02)00236-1

Cited by 39 publications

(29 citation statements)

References 42 publications

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“…In LL conditions, Q A and the PQ pool are oxidized and the Dd+Dt pool size decreases. As long as Q A is reduced, which in our experiments was induced by adding DCMU or by the sudden transfer of the cells from HL to darkness (Dijkman and Kroon, 2002;Grouneva et al, 2009), there is neither an increase nor a decrease of the Dd+Dt pool size independent of the oxidation state of the PQ pool.…”

Section: The Regulation Of Lhcx Gene Expressionmentioning

confidence: 54%

“…In diatoms, a chlororespiration process exists that influences the redox state of the PQ pool in the dark (Jakob et al, 1999;Dijkman and Kroon, 2002;Wilhelm et al, 2006;Grouneva et al, 2009). As we saw a clear difference in the Dd+Dt pool size during dark or LL recovery, 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) was utilized to modify the PQ pool redox state.…”

Section: Influence Of Dithiothreitol and Cycloheximide On Npq Dd+dtmentioning

confidence: 99%

“…In diatoms, the involvement of photoreceptors in photosynthetic regulatory processes has been revealed recently (Depauw et al, 2012), while redox regulation via thioredoxins seems to be of minor importance for the regulation of the Calvin cycle (Kroth et al, 2008), even if thioredoxins may control the activity of carboanhydrases in the pyrenoid (Kikutani et al, 2012). The redox state of the PQ pool is of crucial importance in several photosynthetic regulatory processes, such as the PSII electron cycle (Onno Feikema et al, 2006;Lavaud et al, 2007), nonphotochemical fluorescence quenching (NPQ; Lavaud, 2007;Lavaud et al, 2007), and chlororespiration (Caron et al, 1987;Dijkman and Kroon, 2002;Grouneva et al, 2009). The dynamics of the PQ pool redox state as a function of light intensity differ between diatom species and between diatoms and land plants (Ruban et al, 2004;Lavaud, 2007;Materna et al, 2009).…”

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confidence: 99%

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High Light Acclimation in the Secondary Plastids Containing Diatom Phaeodactylum tricornutum is Triggered by the Redox State of the Plastoquinone Pool

et al. 2012

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In diatoms, the process of energy-dependent chlorophyll fluorescence quenching (qE) has an important role in photoprotection. Three components are essential for qE: (1) the light-dependent generation of a transthylakoidal proton gradient; (2) the deepoxidation of the xanthophyll diadinoxanthin (Dd) into diatoxanthin (Dt); and (3) specific nucleus-encoded antenna proteins, called Light Harvesting Complex Protein X (LHCX). We used the model diatom Phaeodactylum tricornutum to investigate the concerted light acclimation response of the qE key components LHCX, proton gradient, and xanthophyll cycle pigments (Dd+Dt) and to identify the intracellular light-responsive trigger. At high-light exposure, the up-regulation of three of the LHCX genes and the de novo synthesis of Dd+Dt led to a pronounced rise of qE. By inhibiting either the conversion of Dd to Dt or the translation of LHCX genes, qE amplification was abolished and the diatom cells suffered from stronger photoinhibition. Artificial modification of the redox state of the plastoquinone (PQ) pool via 3-(3,4-dichlorophenyl)-1,1-dimethylurea and 5-dibromo-6-isopropyl-3-methyl-1,4-benzoquinone resulted in a disturbance of Dd+Dt synthesis in an opposite way. Moreover, we could increase the transcription of two of the four LHCX genes under low-light conditions by reducing the PQ pool using 5-dibromo-6-isopropyl-3-methyl-1,4-benzoquinone. Altogether, our results underline the central role of the redox state of the PQ pool in the light acclimation of diatoms. Additionally, they emphasize strong evidence for the existence of a plastid-to-nucleus retrograde signaling mechanism in an organism with plastids that derived from secondary endosymbiosis.

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Section: The Regulation Of Lhcx Gene Expressionmentioning

confidence: 54%

Section: Influence Of Dithiothreitol and Cycloheximide On Npq Dd+dtmentioning

confidence: 99%

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confidence: 99%

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High Light Acclimation in the Secondary Plastids Containing Diatom Phaeodactylum tricornutum is Triggered by the Redox State of the Plastoquinone Pool

et al. 2012

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“…Under environmental stress conditions, such as salinity and high irradiance, plants show the ingenious adaptations at all levels of organization, from morphological to biochemical, molecular, and physiological levels (Diaz et al, 2007). At the biochemical and physiological levels, plants can alter their flow of photosynthetic electron transfer in several ways in order to cope with the stress (Dijkman and Kroon, 2002;Quiles, 2006;Diaz et al, 2007;Gamboa et al, 2009;Ibanez et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In some conditions such as stress, there is an alternative route to the typical electron transport chain, namely chlororespiration, which consists of the electron transfer to an oxygen molecule through the plastoquinone (PQ) pool through a terminal oxidase and the generation of a water molecule at the thylakoid stromal side (Bennoun, 1982;Nixon, 2000;Dijkman and Kroon, 2002;Peltier and Cournac, 2002;Rumeau et al, 2007). The existence of such pathway has been molecularly confirmed by the discovery of new molecular components in the thylakoid membranes, including a plastid-encoded NAD(P)H dehydrogenase complex -NDH (Quiles and Cuello, 1998;Rumeau et al, 2005), and a nucleus-encoded plastid terminal oxidase -PTOX (Aluru and Rodermel, 2004;Kuntz, 2004).…”

Section: Introductionmentioning

confidence: 99%

Effects of n-propyl gallate on photosynthesis and physiological parameters in Dunaliella salina are affected by stressful conditions

Einali

Shariati

2012

Braz. J. Plant Physiol.

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We studied the effects of n-propyl gallate, which is a plastid terminal oxidase inhibitor involved in chlororespiration, on photosynthesis and physiological parameters in Dunaliella salina grown under different salinities and under low or high irradiance using chlorophyll a fluorescence transient measurements and pigment analysis. The inhibitor up to 1 mM had an additive significant effect on the photosynthetic efficiency in the cell suspensions grown under low salinity and irradiance. However, in the presence of high n-propyl gallate concentration (4 mM), there was a negative effect on all physiological aspects. In contrast, this high concentration of the inhibitor could enhance efficiency of electron transport and growth parameters under high irradiance. On the other hand, with salinity increase, the unfavorable effects of high inhibitor concentration on the efficiency of photosystem II were less evident than of low salinity. Interestingly, n-propyl gallate high concentration had a positive effect on fluorescence and on physiological parameters when high salinitiesgrown cells were exposed to high irradiance. The results suggest that there is a rational correlation between increase of salinity and algae ability to bypass n-propyl gallate inhibited plastid terminal oxidase function and also direct influence of its lethal concentration on photosystem II compartment. The ability is especially substantial when the increase of salinity is accompanying high irradiance. Furthermore, these data show that algal responses to inhibitor concentrations are different under various environmental conditions.

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An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms

Thomalla

Moutier

Ryan‐Keogh

et al. 2017

Limnology & Ocean Methods

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Autonomous platforms will begin to address the space‐time gaps required to improve estimates of phytoplankton distribution, which will aid in the quantification of baseline conditions necessary to detect long‐term trends that can be attributed to factors such as climate change. However, there is a need for high quality controlled and verified datasets. In vivo fluorescence provides a proxy for chlorophyll pigment concentration, but it is sensitive to physiological downregulation under incident irradiance (fluorescence quenching). Quenching can undermine the validity of these datasets by underestimating daytime fluorescence derived chlorophyll across regional and temporal scales. Existing methods from the literature have corrected for quenching, however, these methods require certain assumptions to be made that do not hold true across all regions and seasons. The method presented here overcomes some of these assumptions to produce corrected surface fluorescence during the day that closely matched profiles from the previous (or following) night, decreasing the difference to less than 10%. This method corrects daytime quenched fluorescence using a mean nighttime profile of the fluorescence to backscattering ratio multiplied by daytime profiles of backscattering from the surface to the depth of quenching (determined as the depth at which the day fluorescence profile diverges from the mean night profile). This method was applied to a 7‐month glider time series in the sub‐Antarctic Southern Ocean together with four other methods from the literature for comparison. In addition, the method was applied to a glider time series from the North Atlantic to demonstrate its applicability to other ocean regions.

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Indications for chlororespiration in relation to light regime in the marine diatom Thalassiosira weissflogii

Cited by 39 publications

References 42 publications

High Light Acclimation in the Secondary Plastids Containing Diatom Phaeodactylum tricornutum is Triggered by the Redox State of the Plastoquinone Pool

High Light Acclimation in the Secondary Plastids Containing Diatom Phaeodactylum tricornutum is Triggered by the Redox State of the Plastoquinone Pool

Effects of n-propyl gallate on photosynthesis and physiological parameters in Dunaliella salina are affected by stressful conditions

An optimized method for correcting fluorescence quenching using optical backscattering on autonomous platforms

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