Background: The study was aimed to elucidate the species taxonomy, clinical manifestations, virulence gene profiles and antimicrobial susceptibilities of Aeromonas strains isolated from life-threatening bacteremia in southeastern China. Methods: Clinical samples of Aeromonas causing bacteremia were isolated from a teaching hospital in Wenzhou during 2013 to 2018 and retrospective cohort study was performed. Aeromonas strains were identified to species level by housekeeping gene gyrB. Six virulence-associated genes (aer, lip, hlyA, alt, ast, and act) were screened by polymerase chain reaction (PCR) and antibiotics susceptibility testing (AST) was performed by VITEK 2 Compact system.Results: A total of 58 patients with bacteremia caused by Aeromonas species were collected during 6 years (2013-2018). 58 isolates were identified to five different species, where Aeromonas dhakensis appeared to be predominant (26/58), followed by Aeromonas veronii (13/58), Aeromonas caviae (10/58), Aeromonas hydrophila (7/58) and Aeromonas jandaei (2/58). 16 of 58 patients had poor prognosis. Poor prognosis was significantly associated with community-acquired infections and liver cirrhosis. The progression of bacteremia caused by Aeromonas was extremely fast, especially in A. dhakensis infections. Virulence genes aer, lip, hlyA, alt, ast, and act, were detected at ratios of 24.1% (14/58), 62.1% (36/58), 65.5% (38/58), 58.6% (34/58), 15.5% (9/58) and 65.5% (38/58), respectively. Antimicrobials susceptibility exhibited that 9 out of 58 isolates were identified as multi-drug resistant (MDR) organism. The majority of Aeromonas strains maintained susceptible to 3rd generation cephalosporins, aminoglycosides, fluoroquinolones and furantoin. Conclusions: The prevalence and dangerousness of Aeromonas infections, especially A. dhakensis, are underestimated in clinic. Continuous monitoring is essential to keep track of MDR Aeromonas due to the increasing prevalence recently and more effective measure is required to control the spread of resistance determinants.