Increasing of Laccase and Manganese Peroxidase Activity by Co-Culture of Immobilized Pleurotus ostreatus and Lentinus tigrinus Mycelia

Abstract

In this study it has firstly reported the using of immobilized fungal mycelia in fungal co-culture studies to enhance activity of lignin-modifying enzymes. For this purpose, the mycelia of Pleurotus ostreatus OBCC 6043 and Lentinus tigrinus OBCC 3007 were immobilized on nylon scouring pad as carrier material. The immobilized mycelia of the fungi were compared n terms of their laccase and manganese peroxidase activities in mono-and co-culture conditions. The maximum laccase activities of pure Pleurotus ostreatus OBCC 6043 and Lentinus tigrinus OBCC 3007 cultures were determined as 53.73 and 27.58 U/L, respectively, while the maximum manganese peroxidase activities were 12.54 and 52.02 U/L. In co-culture conditions, distinct enhancement was observed in laccase and manganese peroxidase activities as 319.28 and 554.33 U/L, respectively. In the case of laccase, enzyme activity was 5.94 and 11.58 times higher than that of Pleurotus ostreatus OBCC 6043 and Lentinus tigrinus OBCC 3007 mono-cultures, respectively. On the other hand, manganese peroxidase activity could be improved distinctly, 44.21 and 10.66 fold higher values than the corresponding ones. The results of the present co-culture study are significantly higher than most of the reported results in the literature, not only for laccase but also for manganese peroxidase activity.

Antagonostic effectiveness of Macromycetes against Candida albicans strains and Issatchenkia orientalis

“…Obtained from C. comatus mycelia (strain JT-01) laccase was shown previously as antipathogenic protein that possessed antiproliferative and HIV-1 reverse transcriptase inhibitory activities (Zhao et al 2014). The enhanced activity of lignin-modifying enzymes (particular laccase, manganese peroxidase, phenol- oxidase and peroxidase activities) in fungal coculture and the important role of these enzymes in the antagonistic interaction between species pointed out by different researchers (Li 1981;1983;Bruce and Highley 1991;Nia 1992;Freitag and Morrel 1992;Rayner et al 1994;Savoie et al 1998;Hatvani et al 2002;Badalyan et al 2002;Uzar et al 2017). In addition, dark zones can be due to melanin production.…”