Increased Susceptibility to Staphylococcus aureus Colonization of the Skin of the NOA mouse: A Potentially Useful Animal Model for Evaluating Antiseptic Effects

Kondo, Taizo; Ohno, Hitoshi; Taguchi, Keisuke; Satode, Ryotaro; Kondo, Toshio; Shiomoto, Yasuhisa

doi:10.1538/expanim.55.49

Cited by 4 publications

(2 citation statements)

References 13 publications

(19 reference statements)

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“…To further clarify if loss of Sa3mw increased the rate of colonization by S. aureus, we used an NOA mouse model that develops spontaneous dry skin and lesions similar to atopic dermatitis (20) in addition to using BALB/c and BALB/c-nu/nu mice. The mice were subjected to inoculation of S. aureus onto their ear skin, using strains MW2 and MW2⌬Sa3mw, the latter of which had been isolated from the ear of BALB/c-nu/nu mice after MW2 colonization as described above.…”

Section: Resultsmentioning

confidence: 99%

“…BALB/cAnNCrlCrlj (BALB/c) and BALB/cAnNCrlCrlj-nu/nu (BALB/c-nu/nu) mice (female, 6 weeks old, with no superficial dermatitis) and Naruto Research Institute Otsuka Atrichia (NOA) mice (male, 6 weeks old, with dry skin) were purchased from SLC (Shizuoka, Japan) (20). The mice were housed in individually ventilated cages (IVC) (Techniplast, Buguggiate, Italy) under specificpathogen-free conditions.…”

Section: Methodsmentioning

confidence: 99%

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Beta-Hemolysin Promotes Skin Colonization by Staphylococcus aureus

Katayama

Baba

Sekine

et al. 2013

Journal of Bacteriology

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Colonization by Staphylococcus aureus is a characteristic feature of several inflammatory skin diseases and is often followed by epidermal damage and invasive infection. In this study, we investigated the mechanism of skin colonization by a virulent community-acquired methicillin-resistant S. aureus (CA-MRSA) strain, MW2, using a murine ear colonization model. MW2 does not produce a hemolytic toxin, beta-hemolysin (Hlb), due to integration of a prophage, Sa3mw, inside the toxin gene (hlb). However, we found that strain MW2 bacteria that had successfully colonized murine ears included derivatives that produced Hlb. Genome sequencing of the Hlb-producing colonies revealed that precise excision of prophage Sa3mw occurred, leading to reconstruction of the intact hlb gene in their chromosomes. To address the question of whether Hlb is involved in skin colonization, we constructed MW2-derivative strains with and without the Hlb gene and then subjected them to colonization tests. The colonization efficiency of the Hlb-producing mutant on murine ears was more than 50-fold greater than that of the mutant without hlb. Furthermore, we also showed that Hlb toxin had elevated cytotoxicity for human primary keratinocytes. Our results indicate that S. aureus Hlb plays an important role in skin colonization by damaging keratinocytes, in addition to its well-known hemolytic activity for erythrocytes.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%