Increased secretory activity and estradiol receptor expression are among other relevant aspects of MCF-7 human breast tumor cell growth which are expressed only in the absence of serum

Medrano, Estela E.; Resnicoff, Mariana; Cafferata, Eduardo G.; Larcher, Fernando; Podhajcer, Osvaldo L.; Bover, Laura; Molinari, Beatriz L.

doi:10.1016/0014-4827(90)90270-k

Cited by 9 publications

(3 citation statements)

References 17 publications

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“…These cells maintained the expression of nuclear progesterone receptors and their amounts were down-regulated by progesterone as demonstrated by others [43]. The use of a serum-free medium in this model gave an interesting result as serum contains many substances which never come into contact with most body cells [15,27] and therefore can modify the phenotypic behaviour of the cells in vitro [42,46].…”

Section: Introductionmentioning

confidence: 72%

Serum‐free culture of stromal and functionally polarized epithelial cells of guinea‐pig endometrium: a potential model for the study of epithelial‐stromal paracrine interactions

Mahfoudi

Fauconnet

Bride

et al. 1992

Biology of the Cell

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Stromal and glandular epithelial (GE) cells were isolated from guinea-pig endometrium and growth to near confluency (6-8 days) in primary culture on plastic surfaces in a serum-supplemented medium (SSM). The stromal cells were subcultured on plastic dishes and maintained for 72 h in SSM. Then SSM was replaced by a chemically defined medium (CDM) and the stromal cells grown to confluency (5-7 days). The GE cells were subcultured in CDM, on a basement membrane matrix (Matrigel) applied to permeable Millicell-PC filters, and grown to confluency (5 days). Homogeneity of the subcultured endometrial cell populations was ascertained immunocytochemically. The filter-cultured GE monolayers were polarized morphologically, and displayed epithelial-specific specialized structures. These monolayers had functional tight junctions as verified by a measurable transepithelial resistance. The subcultured cell populations were distinguished by an analysis of their cellular and secretory proteins after labelling with [35S]-methionine and analysis by polyacrylamide gel electrophoresis. The filter-cultured GE monolayers allowed identification of the proteins released vectorially in the apical or the basal secretory compartment, thus demonstrating the functional polarization of GE cells in this bicameral culture system. Within the defined conditions of this culture system, the paracrine factors released by the two endometrial cell populations as well as the interplay of stromal-epithelial interactions and ovarian hormones could be investigated.

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Section: Introductionmentioning

confidence: 72%

Serum‐free culture of stromal and functionally polarized epithelial cells of guinea‐pig endometrium: a potential model for the study of epithelial‐stromal paracrine interactions

Mahfoudi

Fauconnet

Bride

et al. 1992

Biology of the Cell

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“…However, the extracellular domain of the targeted protein may be shed, thereby removing the target from the tumor (38). In addition, monoclonal antibodies are large and may not arrive at the tumor site or may not be able to penetrate past the outer layers of tumor.…”

Section: New Therapeuticsmentioning

confidence: 99%

“…However, not all patients with erbB overexpression benefit from these therapies for several reasons (37). It is known that the extracellular domain of HER-2, where trastuzamab is targeted, is shed thereby removing the target from the tumor (38). In addition, monoclonal antibodies are large and may not arrive at the tumor site or may not be able to penetrate past the outer layers of tumor.…”

Section: Erbb Familymentioning

confidence: 99%

Why should we still care about oncogenes?

Diehl¹,

Keller²,

Ignatoski³

2007

Molecular Cancer Therapeutics

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Although oncogenes and their transformation mechanisms have been known for 30 years, we are just now using our understanding of protein function to abrogate the activity of these genes to block cancer growth. The advent of specific small-molecule inhibitors has been a tremendous step in the fight against cancer and their main targets are the cellular counterparts of viral oncogenes. The best-known example of a molecular therapeutic is Gleevec (imatinib). In the early 1990s, IFN-α treatment produced a sustained cytologic response in ∼33% of chronic myelogenous leukemia patients. Today, with Gleevec targeting the kinase activity of the proto-oncogene abl, the hematologic response rate in chronic myelogenous leukemia patients is 95% with 89% progression-free survival at 18 months. There are still drawbacks to the new therapies, such as drug resistance after a period of treatment, but the drawbacks are being studied experimentally. New drugs and combination therapies are being designed that will bypass the resistance mechanisms. [Mol Cancer Ther 2007;6(2):418–27]

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