Increased Paxillin expression in prostate cancer is associated with advanced pathological features, lymph node metastases and biochemical recurrence

Abstract

Purpose Paxillin regulates cell-cell adhesion, and altered Paxillin expression has been associated with human carcinogenesis. This study analyzed the association between Paxillin expression in prostate cancer (PCa) tissues with clinicopathological features, lymph node metastasis and biochemical PCa recurrence.Methods A total of 386 tissue specimens from PCa patients who received radical prostatectomy and 60 tissue specimens from benign prostatic hyperplasia (BPH) cases were collected to construct tissue microarrays, which were subsequently immunostained for Paxillin expression. Thirty positive lymph node tissue specimens and 10 healthy prostate tissue specimens were randomly selected for Paxillin immunostaining.Results The association between Paxillin expression, lymph node metastasis and biochemical PCa recurrence was analyzed. Paxillin expression was significantly higher in PCa than both normal and BPH tissues (P<0.001) and was correlated with preoperative prostate-specific antigen level, Gleason score, clinical tumor stage, lymph node metastasis, positive surgical margin, extracapsular extension and seminal vesicle invasion (P<0.05 for all). Logistic regression analysis showed that Paxillin and Gleason score were independent risk factors for PCa lymph node metastasis (P<0.05). The receiver operating characteristic (ROC) curve indicated that Paxillin expression (AUC=0.723) more accurately predicted PCa lymph node metastasis than Gleason score (AUC=0.692). Kaplan-Meier curve analysis showed that increased Paxillin expression was associated with shortened biochemical-free survival (BFS) after radical prostatectomy (P<0.001).Conclusion Paxillin was significantly upregulated in PCa compared with BPH and normal tissues and associated with lymph node metastasis and shortened BFS of PCa. Further study will investigate the underlying molecular mechanism and the role of Paxillin in PCa.

Clinicopathological Analysis of the ISUP Grade Group And Other Parameters in Prostate Cancer: Elucidation of Mutual Impact of the Various Parameters

“…It was also found that isolated carcinoma cells were not evident in the metastatic foci in the lymph nodes; thus, showing some degree of aggregation. Expression of paxillin, reported in prostate cancer ( 41 ) and involved in cancer cell aggregation ( 42 ), could be implicated to this observation; further studies are needed to clarify this relationship. The glomeruloid pattern was relatively rare in the present study.…”

Downregulation of Snail by DUSP1 Impairs Cell Migration and Invasion through the Inactivation of JNK and ERK and Is Useful as a Predictive Factor in the Prognosis of Prostate Cancer

“…In this regard, DUSP22, a member of the DUSP1 family which reduces JNK activation, negatively regulates cell migration through FAK dephosphorylation and inactivation in lung cancer cells [ 57 ]. Given that FAK and paxillin expression is elevated in prostate cancer and both proteins are associated with tumor progression, lymph node metastasis, and/or shortened survival [ 58 , 59 ], it is also plausible that in our cancer model, the paxillin/FAK pathway could contribute to the regulation of Snail expression by ERK and JNK. However, due to the difference between ERK- and JNK-dependent mechanisms, further research is required to investigate the molecular mechanisms underlying Snail regulation by these kinases.…”

Up‐regulation of LIMK1 expression in prostate cancer is correlated with poor pathological features, lymph node metastases and biochemical recurrence

“…Any discrepancies were resolved by re‐reviewing the sections. Two semi‐quantitative methods and the total LIMK1 immunostaining score methods including staining intensity and the proportion of positive cells were described as follows . The immunohistochemistry score of LIMK1 consists of two parts, including staining intensity and the proportion of positive cells.…”