Increased macromolecular damage due to oxidative stress in the neocortex and hippocampus of WNIN/Ob, a novel rat model of premature aging

Sinha, Jitendra Kumar; Ghosh, Shampa; Swain, Umakanta; Giridharan, Nappan Veettil; Raghunath, Manchala

doi:10.1016/j.neuroscience.2014.03.040

Cited by 41 publications

(29 citation statements)

References 48 publications

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“…According to this theory; free radicals play an important role in impaired redox homeostasis. Cellular macromolecules such as proteins, lipids, carbohydrates, and DNA are vulnerable to free radicals and free radical‐derived products (Kirkwood, ; Ray, Huang, & Tsuji, ; Sinha, Ghosh, Swain, Giridharan, & Raghunath, ). The excessive production of the free radicals and the diminished activity of the antioxidant enzymes and molecules lead to macromolecular cellular damage (Lushchak, Gospodaryov, Yurkevych, & Storey, ).…”

Section: Introductionmentioning

confidence: 99%

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How various concentrations of nutrients affect basal redox homeostasis of fruit flies according to gender and aging

2018

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We evaluated the effects of type of nutrients on redox status according to age and gender in Drosophila melanogaster. Flies were raised on different dose of sucrose and fixed concentration of protein containing media and separated according to their gender and age. Redox status biomarkers such as protein carbonyl groups, dityrosine, kynurenine, lipid hydroperoxides, advanced glycation end products, and Cu,Zn superoxide dismutase were assessed. We found that all of the males had higher levels of basal oxidative stress biomarkers except protein carbonyl. On the other hand, Cu,Zn‐superoxide dismutase activity was elevated. We found that high concentration of sucrose leads to impaired redox status. We also observed that high protein content in sucrose‐based media fecundity rate was increased and also transition to adult flies and survival ratio were decreased. Further studies are needed to focus on evaluation of the relation between median life span, nutritional mechanisms, and redox status biomarkers. Practical applications Aging is characterized by functional decline in whole body system. Unfortunately, many of the life span extension strategies may lead to age‐related disorders. It is widely accepted that impaired redox homeostasis is one of the signs of age‐related pathophysiological changes. Due to difficulties in the establishment and validation of mimetic aging models in rodents, D. melanogaster is becoming/getting widely used model organism in gerontological studies. Currently, high protein and low carbohydrate‐based nutrition regimes have become one of the most popular nutritional choice in aging population. Favorable nutrition type is considered one of the important factors for preventing age‐related pathologies and promote healthy aging. In this study, we aimed to evaluate redox modulatory effects of different nutrition types as combination of various sucrose and constant protein concentration on basal redox status in D. melanogaster. Due to evolutionally conservation, we hypothesize that similar findings may be observed in aging population. Favorable nutrition type may ameliorate or postpone age‐related degenerative processes and promote healthy aging.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

How various concentrations of nutrients affect basal redox homeostasis of fruit flies according to gender and aging

2018

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“…Considering that earlier studies have reported association of oxidative stress with obesity and insulin resistance , we looked into the levels of oxidative stress and antioxidant defense in our model. Moreover, evidence from available literature suggests that vitamin B12 acts as a potential antioxidant .…”

Section: Discussionmentioning

confidence: 99%

Chronic transgenerational vitamin B12 deficiency of severe and moderate magnitudes modulates adiposity—probable underlying mechanisms

et al. 2017

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We have demonstrated previously that severe but not moderate vitamin B12 deficiency altered body composition and induced adiposity in female C57BL/6 mice. This study aims to elucidate the effects of chronic transgenerational dietary vitamin B12 restriction on body composition and various biochemical parameters in the F1 generation offspring of our mouse models of severe and moderate vitamin B12 deficiency established earlier. Female weanling C57BL/6 mice received, ad libitum, for 4 weeks a (i) control diet, (ii) vitamin B12-restricted diet with pectin as dietary fiber (severely deficient diet), or (iii) vitamin B12-restricted diet with cellulose as dietary fiber (moderately deficient diet) and then mated with control males. The offspring of control and severely deficient dams continued on the respective diets of their mothers. Few moderately deficient dams were rehabilitated to control diet from parturition and their pups were weaned to control diet. Also, some offspring born to moderately B12 deficient dams were weaned to control diet, while others continued on the same diet as their mothers. Various parameters were determined in the F1 offspring after 12 and 36 weeks of feeding. The results indicate that both severe and moderate maternal vitamin B12 restrictions were associated with accelerated catch-up growth, increased body fat percentage, visceral adiposity, dyslipidemia, fasting hyperglycemia and insulin resistance in the F1 offspring. Inflammation, increased glucocorticoid and oxidative stress and poor antioxidant defence probably underlie these adverse effects. Rehabilitation from parturition but not weaning was beneficial in delaying the onset of the adverse outcomes in the offspring. © 2016 BioFactors, 43(3):400-414, 2017.

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“…The Long-term mechanisms underlying this association include chronic neuro inflammation [see review of 50], neuroendocrine dysregulation such as leptin and insulin resistance [see review of 51], oxidative stress [52], and synaptic dysfunction[53]. The indirect mechanisms include the vascular damage and BBB breakdown [54], and likely a combination of neuroanatomical changes and biochemical alterations.…”

Section: Discussionmentioning

confidence: 99%

Genetic variability to diet-induced hippocampal dysfunction in BXD recombinant inbred (RI) mouse strains

Xue¹,

Li²,

Yan

et al. 2015

Behavioural Brain Research

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Evidence has emerged suggesting that diet-induced obesity can have a negative effect on cognitive function. Here, we exploited a mouse genetic reference population to look for the linkage between these two processes on a genome-wide scale. The focus of this report is to determine whether the various BXD RI strains exhibited different behavioral performance and hippocampal function under high fat dietary (HFD) condition. We quantified genetic variation in body weight gain and consequent influences on behavioral tests in a cohort of 14 BXD strains of mice (8–12 mice/strain, n=153), for which we have matched data on gene expression and neuroanatomical changes in the hippocampus. It showed that BXD66 was the most susceptible, whereas BXD77 was the least susceptible strain to dietary influences. The performance of spatial reference memory tasks was strongly correlated with body weight gain (P<0.05). The obesity-prone strains displayed more pronounced spatial memory defects compared to the obesity-resistant strains. These abnormalities were associated with neuro inflammation, synaptic dysfunction, and neuronal loss in the hippocampus. The biological relevance of DSCAM gene polymorphism was assessed using the trait correlation analysis tool in Genenet work. Further more, a significant strain-dependent gene expression difference of DSCAM was detected in the hippocampus of obese BXD strains by real-time quantitative PCR. In conclusion, a variety of across-strain hippocampal alterations and genetic predispositions to diet-induced obesity were found in a set of BXD strains. The obesity-prone and obesity-resistant lines we have identified should be highly useful to study the molecular genetics of diet-induced cognitive decline.

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Increased macromolecular damage due to oxidative stress in the neocortex and hippocampus of WNIN/Ob, a novel rat model of premature aging

Cited by 41 publications

References 48 publications

How various concentrations of nutrients affect basal redox homeostasis of fruit flies according to gender and aging

How various concentrations of nutrients affect basal redox homeostasis of fruit flies according to gender and aging

Chronic transgenerational vitamin B12 deficiency of severe and moderate magnitudes modulates adiposity—probable underlying mechanisms

Genetic variability to diet-induced hippocampal dysfunction in BXD recombinant inbred (RI) mouse strains

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