Increased HLA-DR Expression by Enterocytes in Children With Celiac Disease

Arnaud-Battandier, F; Cerf‐Bensussan, Nadine; Amsellem, R.; Schmitz, J

doi:10.1016/s0016-5085(86)80018-x

Cited by 71 publications

(22 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Only a few or no IL-2R positive cells were observed in this group of patients (0-9 (0 6), range 0-2) and in two cases with glandular metaplastic epithelium positive for HLA-DR (one IL-2R positive cells in each case). Again, no statistical differences were shown in terms of OKT3 positive cells in respect of both normal controls and those with wart virus infection (85 (7), range 78-90).…”

Section: Mixed Epithelial Non Viral Disease (Menvd)mentioning

confidence: 77%

Human cervical epithelial cells that express HLA-DR associated with viral infection and activated mononuclear cell infiltrate.

Fais

Fratte²,

Mancini³

et al. 1991

J Clin Pathol

View full text Add to dashboard Cite

The association between the expression of HLA-DR antigens on cervical epithelium and the local immune state of activation in colposcopically obtained biopsy specimens from The purpose of this study was to investigate the association between the expression of HLA-DR antigens on cervical epithelium and the local immune state of activation in colposcopically obtained biopsy specimens from patients with histologically documented wart virus infection and from controls. MethodsTissue samples were obtained from 36 subjects undergoing colposcopy. Cytological and histological analyses of punch biopsy specimens were performed. There were 10 subjects with normal colposcopic, cytological, and histological appearances, 12 with evidence of mixed epithelial non-viral disease (MENVD), and 14 with wart virus infection.Diagnosis of wart virus infection was based on the evidence of flat condyloma. Flat condyloma appeared at colposcopy after treatment of the cervix with acetic acid as raised areas (outside the transformation zone) of shiny, white epithelium with a rough surface and with a punctation or mosaic-like vascular pattern. The diagnosis was confirmed in all 14 cases by histology and cytology, showing koilocytic cells and dyskeratotic cells without evidence of dysplasia.2225Tissue samples were oriented, embedded in Tissue Tek OCT compound (Miles Scientific, USA), snap frozen in liquid nitrogen, and stored at -80°C until used.At least six frozen sections, 5 gm thick, for each specimen were cut on a cryostat, air dried, and fixed in absolute ethanol for 10 minutes at 4°C. Frozen sections were then stained by an indirect immunofluorescence technique, as previously described.6 Briefly, contiguous sections for each specimen were stained for HLA-DR and IL-2R and incubated with the optimal dilutions of each of the monoclonal antibodies listed below for one hour at 4°C. Additional sections were incubated with the OKT3 monoclonal antibody. Sections were then washed twice with PBS and incubated with a fluorescein isothiocyanate-conjugated goat-antimouse antibody for 30 minutes at room temperature. Sections were then washed three

show abstract

Section: Mixed Epithelial Non Viral Disease (Menvd)mentioning

confidence: 77%

Human cervical epithelial cells that express HLA-DR associated with viral infection and activated mononuclear cell infiltrate.

Fais

Fratte²,

Mancini³

et al. 1991

J Clin Pathol

View full text Add to dashboard Cite

show abstract

“…It is likely that the increased production of interferon gamma, which is associated with active coeliac disease, is responsible for this effect, and other studies have reported the rapid appearance of interferon gamma mRNA in in vitro experiments [32,33]. In coeliac disease, the increased intensity of MHC class II staining and the presence of this molecule on crypt enterocytes is very characteristic of active disease [12][13][14]. These features were absent in all the biopsies examined here, both before and after oats ingestion.…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies have shown that in normal duodenal tissue, HLA‐DR staining is observed on the upper half of villi, whereas in active coeliac disease staining of the entire villus and crypt enterocytes is found [11–13] . A three‐point semiquantitative scale of staining was established: 1+ to indicate weak staining of tips of villi; 2+ to indicate moderate staining of the upper half of villi; and 3+ to indicate staining of entire villi and also the crypt enterocytes.…”

Section: Methodsmentioning

confidence: 99%

Immunohistochemical analysis of coeliac mucosa following ingestion of oats

Srinivasan

Jones

Carolan

et al. 2006

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SummaryThere is now considerable clinical evidence that oats do not activate coeliac disease. Nonetheless, a reluctance to include oats in the gluten-free diet remains. Because gluten-induced damage is accompanied by activation of the gastrointestinal immune system, the purpose of this study was to investigate if similar changes were induced by oats ingestion. Small intestinal histological sections from 10 patients who ingested 50 g of oats daily for 3 months were investigated for possible evidence of immune activation. Tissue obtained before and after oats challenge was stained with a series of antibodies directed against the following molecules: human leucocyte antigen D-related (HLA-DR), Ki-67, CD25, CD54 [intercellular adhesion molecule 1 (ICAM-1)] and mast cell tryptase. None of the patients developed clinical or laboratory evidence of adverse effects. The distribution of intestinal HLA-DR expression was not affected by oats ingestion and the crypt epithelium remained unstained. In the pre-oats biopsies, the percentage of Ki-67 positive enterocytes, 29·5 ± ± ± ± 6·9 [95% confidence interval (CI) 13·9-45·0] did not differ significantly from that found in postoats biopsies, 41·2 ± ± ± ± 3·7 (95% CI, 32·8-49·6), P = = = = 0·19, not significant. Furthermore, oats ingestion did not alter the number of CD25 positive and tryptase positive cells. Finally, the distribution and intensity of ICAM-1 staining was unchanged by dietary oats. In summary, detailed immunohistological studies of biopsies from patients ingesting oats for 3 months did not reveal evidence of immune activation. Together with other reported findings, this study strengthens the view that oats can be included safely in the diet of gluten sensitive patients.

show abstract

“…When the patients are on a normal gluten-containing diet, a strong expression of HLA-DR along the whole lengths of the crypts can be shown (Fig. On a gluten-free diet, the expression of HLA-DR in the crypts does not differ from that seen in controls (14,43). 4).…”

Section: Hla Antigen Expression In Epithelial Cellsmentioning

confidence: 97%