2019
DOI: 10.1371/journal.pone.0223616
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Abstract: Since the discovery that entomopathogenic fungi can live inside plants as endophytes, researchers have been trying to understand how this affects mainly plants and herbivores. We studied how inoculation of Vicia faba L. (Fabales: Fabaceae) plants with Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Ascomycota: Hypocreales) (strain GHA) either via the seeds or leaves influenced the nymph production of two successive generations of Aphis fabae Scopoli (Hemiptera: Aphididae). While we did not find any difference… Show more

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Cited by 24 publications
(20 citation statements)
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“…bassiana strain GHA (BotaniGard) was spread on potato dextrose agar (PDA). After 2–3 weeks (20°C, darkness) the fungus was harvested in a 0.2% Tween20 solution using an inoculation spreader (Sarstedt A/S) to scrape the surface of the fungus culture as described in [ 15 ]. The spore suspension was prepared by centrifuging the collected fungal material two times at 4,000 rpm (3076 x g) for 4 minutes, thereafter adjusting the concentration to 10 7 spores/ml by spore counting in a Fuchs-Rosenthal counting chamber.…”
Section: Methodsmentioning
confidence: 99%
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“…bassiana strain GHA (BotaniGard) was spread on potato dextrose agar (PDA). After 2–3 weeks (20°C, darkness) the fungus was harvested in a 0.2% Tween20 solution using an inoculation spreader (Sarstedt A/S) to scrape the surface of the fungus culture as described in [ 15 ]. The spore suspension was prepared by centrifuging the collected fungal material two times at 4,000 rpm (3076 x g) for 4 minutes, thereafter adjusting the concentration to 10 7 spores/ml by spore counting in a Fuchs-Rosenthal counting chamber.…”
Section: Methodsmentioning
confidence: 99%
“…Vicia faba seeds (cv. ‘Vertigo’) were surface disinfected before sowing by being soaked in 70% ethanol for two minutes, rinsed in sterile water, submerged for two minutes in 1% sodium hypochlorite and finally rinsed three times in sterile water as described in [ 15 ]. To assess the surface sterilization efficacy, 1 mL of the final rinse water was plated on PDA medium.…”
Section: Methodsmentioning
confidence: 99%
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“…The presence of endophytic EPF resulted in the significant reduction of both pest population and plant damage in most cases. However, there have been reports of poor performance, indicating the complexity of these EPF insect-plant interactions [17,56,88,91].…”
Section: Endophytic Epf As Biocontrol Agentsmentioning
confidence: 99%
“…Reduction of larval survival [63] Liriomyza huidobrensis Reduced population [16] Acyrthosiphon pisum Reduced population [56] Aphis fabae Reduced population [56] Increased population [91] common bean Helicoverpa armigera Reduction of larval survival [63] Liriomyza huidobrensis Reduced population [16] Table A2. Cont.…”
Section: Helicoverpa Armigeramentioning
confidence: 99%