Increased expression of long non-coding RNA XIST predicts favorable prognosis of cervical squamous cell carcinoma subsequent to definitive chemoradiation therapy

Abstract

The present retrospective study aimed to examine the association between the expression of long non-protein-coding RNAs (lncRNAs) and clinical prognosis in the pretreatment formalin-fixed, paraffin-embedded (FFPE) tissue samples of cervical squamous cell carcinoma patients that underwent platinum-based chemoradiation therapy. Between 2001 and 2013, 49 consecutive patients with squamous cell cervical carcinoma were selected for the present study (median follow-up period, 44.1 months). The patients possessed an International Federation of Gynecology and Obstetrics stage of IB1/IIA1 (with pelvic lymph node metastasis), IB2 or IIA2-IVA, and had been treated with definitive chemoradiation therapy. The pretreatment FFPE tumor biopsies of the patients obtained diagnosis were used for analysis. Total RNAs were extracted from the FFPE tumor tissues and reverse transcription-quantitative polymerase chain reaction was performed to examine the expression level of lncRNAs. The expression level of X inactive-specific transcript (XIST) demonstrated a significant association with the overall survival rate (P=0.014). The 4-year overall survival rates were 87.1 and 54.4% in the high and low XIST expression groups, respectively. Since the expression of XIST is associated with the overall survival rate, this lncRNA has the potential to become a predictor for the prognosis of cervical squamous cell carcinoma patients that are treated with chemoradiation therapy. Additional studies are required to investigate the underlying mechanisms of XIST that are associated with prognosis.

The prognostic value of long non coding RNAs in cervical cancer: A meta-analysis

“…Besides, XLOC_010588, XIST, LET, MEG3 were correlated to poor prognosis with the low expressions of lncRNAs in cervical cancer. With all the lncRNAs HOTAIR generate the highest HR of 5.28 [ 16 ]; by contrast, XIST exhibited the lowest HR of 0.27 [ 17 ]. Moreover, stratified analyses were performed using studies with the ethnicity.…”

Upregulation of long noncoding RNA XIST is associated with poor prognosis in human cancers

“…In hepatocellular carcinoma, the tumor suppressor activity of XIST was exerted by upregulating phosphatase and tensin homolog deleted on chromosome ten (PTEN) expression via miR‐181a (Chang et al, ), whereas the oncogenic role of XIST was exerted by upregulated MAPK1 expression via miR‐194‐5p (Kong et al, ). The underlying mechanism of XIST as a tumor suppressor in cervical squamous cell carcinoma is unexplored (Kobayashi et al, ); however, its oncogenic role in cervical squamous cell carcinoma was reported to attribute to the upregulation of RNA binding protein FUS through miR‐200a (Zhu et al, ). XIST upregulated programmed cell death protein 4 (PDCD4) expression by interacting with miR‐21‐5p and inhibited osteosarcoma cell growth and metastasis (Zhang & Xia, ).…”

Deciphering the Role of the Barr Body in Malignancy: An insight into head and neck cancer

“…39 In HNSCC cases, Xi reactivation can potentially be considered a marker of heterochromatin instability associated with poor prognosis as, much like cervical cancer, the disease may be associated with epigenetic modifications as well as oncoviruses that could alter the X-linked genes. 11,36,37 Thus, the destabilised genomic repertoire in HNSCC appears to be further undermined by epigenetic events. 39,40 However, before considering an association between the Barr body and HNSCC, a causal relation between X-linked TSGs and HNSCC development must be established.…”

“…7,[11][12][13][14] This article focuses on reviewing variations in Barr body frequency in different malignancies and proposing its hypothetical involvement in HNSCC development. There is a need to further explore the role of sex chromosomes in HNSCC development in order to determine potential clinical implications.…”

“…69 As previously mentioned, increased XIST expression-which contains a long non-coding RNA transcript-has been found to predict a favourable prognosis in cases of cervical squamous cell carcinoma. 11 Since the transcriptional capacity of host cell chromatins can be regulated by HPV E6 and E7 oncoproteins, further research is needed to fully comprehend HPV-induced modulation of long non-coding RNAs. 70 …”