Increased expression of BPI fold-containing family A member 1 is associated with metastasis and poor prognosis in human colorectal carcinoma

Abstract

Abstract. Bactericidal or permeability-increasing protein fold-containing family A member 1 (BPIFA1) has been demonstrated to be involved in inflammatory responses in the upper airway and the progression of non-small cell lung cancer. However, the expression levels of BPIFA1 and its clinical prognostic significance in colorectal carcinoma (CRC) has not yet been elucidated. Reverse transcription-polymerase chain reaction and immunohistochemistry were used to analyze the expression levels of BPIFA1 in CRC and normal mucosal tissues. The associations between BPIFA1 expression levels and clinicopathological characteristics, and its predictive value for prognosis in CRC, were statistically evaluated as appropriate. The expression levels of BPIFA1 were revealed to be upregulated at the transcriptional and translational levels in CRC tissues, compared with in normal mucosal tissues. A high expression level of BPIFA1 is significantly associated with invasion depth (P=0.040), lymph node metastasis (P=0.035) and distant metastasis (P=0.010). Furthermore, Kaplan-Meier analysis indicated that BIPFA1 overexpression is associated with short survival time, and the Cox proportional hazards model of risk analysis indicated that BPIFA1 is an independent prognostic factor for patients with CRC. The results of the present study suggested that BPIFA1 expression is upregulated in CRC tissues, and that an increased expression level of BPIFA1 is associated with tumor invasion, metastasis and poor prognosis, indicating that BPIFA1 may be a potential clinical prognostic predictor and therapeutic target for patients with CRC. IntroductionColorectal carcinoma (CRC) is the one of the most common types of cancer, and is the fifth leading cause of cancer-associated mortalities worldwide (1). Despite recent advancements in the surgical techniques, radiotherapy and chemotherapy available to patients diagnosed with CRC over the past several decades, the overall survival rate has not significantly improved. The existence of numerous known carcinogens and varying genetic backgrounds makes it difficult to determine which factors are most important in the development of CRC. Thus, there is a requirement to further understand the underlying molecular mechanisms and identify novel prognostic biomarkers and therapeutic targets to provide improved treatment strategies for CRC.Bactericidal or permeability-increasing protein fold-containing family A member 1 (BPIFA1) is a protein-coding gene specifically expressed in the upper airways and nasopharyngeal regions. A number of previous studies have demonstrated that BPIFA1 is involved in various physiological and pathological processes (2-19). It is considered to be involved in inflammatory responses to irritants in the upper airway (2-7). BPIFA1 may decrease mycoplasma pneumonia expression levels and inhibit interleukin 8 (8). BPIFA1 protein was also revealed to have antibacterial activity against gram-negative bacteria (9,10). The anti-inflammatory function has been associated with the regulation of macro...

Understanding seasonal weight loss tolerance in dairy goats: a transcriptomics approach

“…It seems therefore, that different inflammatory responses associated genes and proteins are up-regulated in the Majorera breed because of feed restriction. Considering the defense mechanisms pathway, gene SVEP1 is one of the various components of the immune response [44], whereas LOC102187241 codes for protein BPI fold containing family B member 1, a protein of inflammatory response in the upper airway and the progression of non-small cell lung cancer in humans [45]. Such results are difficult to interprete and further studies are needed to better understand these results.…”

Cell Type Catalog of Middle Turbinate Epithelium (Cell Catalog of Turbinate Epithelium)

“…Serous cells secrete zymogens, enzymes, and antibodies to defend airways against bacteria and viruses; these include lysozyme, lactoferrin, zymogen granule protein, and bactericidal permeability‐increasing protein. We identified a large cluster of serous cells characterized by high expression of LYZ , STATH , LTF, ZG16B , BPIFA1, BPIFB1 , S100A1 , and PIP ( Figure 2 ) 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ; the latter 2 play a role in the regulation of calcium content in the secretions. 11 , 13 , 25 …”

“…Serous Cells (4287 Cells, 23.0% of All Cells) Serous cells secrete zymogens, enzymes, and antibodies to defend airways against bacteria and viruses; these include lysozyme, lactoferrin, zymogen granule protein, and bactericidal permeability-increasing protein. We identified a large cluster of serous cells characterized by high expression of LYZ, STATH, LTF, ZG16B, BPIFA1, BPIFB1, S100A1, and PIP (Figure 2) [11][12][13][14][15][16][17][18][19][20][21][22][23][24] ; the latter 2 play a role in the regulation of calcium content in the secretions. 11,13,25 Goblet Cells (384 Cells, 2.1% of All Cells) SPDEF, MUC5B, MUC5AC, FOXA3, TFF1, and TFF3 are known to be expressed in mucus-producing goblet cells of human respiratory epithelia.…”

Cell type catalog of middle turbinate epithelium

“…Serous cells secrete zymogens, enzymes, and antibodies to defend airways against bacteria and viruses; these include lysozyme, lactoferrin, zymogen granule protein, and bactericidal permeability-increasing protein. We identified a large cluster of serous cells characterized by high expression of LYZ, STATH, LTF, ZG16B, BPIFA1, BPIFB1, S100A1 and PIP (Figure 2) [18][19][20][21][22][23][24][25][26][27][28][29][30][31] ; the latter two play a role in regulation of calcium content in the secretions. 18,20,32 Goblet Cells SPDEF, MUC5B, MUC5AC, FOXA3, TFF1 and TFF3 are known to be expressed in mucusproducing goblet cells of human respiratory epithelia.…”